Final Report Summary - CELLPROM (Cell programming by nanoscaled devices)
The aim of the CELLPROM project was to develop new technology equipment in order to study in vitro the cell differentiation stimulated by nanoscaled surfaces as well as to investigate the effects of differently designed nanostructured surfaces on cell differentiation. The work had been concentrated on the differentiation of the Murine embryonic stem cells (MESC), the adult Pancreatic and glandular stem cells (PSLCs) as well as the Murine bone marrow precursor cells (MBMPC). The main objective was to develop procedures and devices for the imprinting of cells via nanoscaled macromolecular landscapes, the nanoscapes, which would allow producing well defined populations of individually programmed cells in a non-invasive way. Although surface imprinting of cells would be realised in artificial nano-biotechnological devices, e.g. nanostructured stamps or beads, these tools have been designed according to the natural principles of cellular signalling and differentiation. As nanocomponents are essential to the imprinting process, suitable techniques and principles to form nanoscaled macromolecular patterns on arbitrary surface geometries had to be developed.