Objective
The goal of the project is the development of a novel analytical approach for rapid comparison of residues- or selenoproteomes of " normal" and " treated" cells. The interest in this topic is driven by the state-of-the-art knowledge of the cellular signal transudation and posttranslational modifications of proteins (involving phosphorylation) and the need for characterisation of selenoproteome in the increasingly used food supplements. The study of these fields depends strongly on the combined application of a powerful separation technique and a sensitive and specific detection of heteroelements (phosphorous, sulphur or selenium). In this project, I would like to develop an efficient interface between capillary liquid chromatography (LC) and inductively coupled plasma mass spectrometry (ICP-MS). This approach will offer a unique way for the quantification of proteins containing a heteroelement, and will allow pinpointing targets for more detailed MALDI-TOF (matrix- assisted laser adsorption ionisation-time-of-flight) MS and ES (electro spray) MS/MS analyses. The latter two mass spectrometric techniques will enable me not only to detect but also to identify the proteins of interest. Therefore any new information gained during the experimental part of the project would contribute to a better understanding of intracellular processes. On the level of applications the project will focus on the optimisation of the characterisation of enzymatic digests of some model phosphoproteins (beta-casein, MAP kinas) and selenoproteins (solemnised yeast sodium codicil suffocate extract).
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences inorganic chemistry noble gases
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences chemical sciences inorganic chemistry alkali metals
- natural sciences chemical sciences organic chemistry amines
- natural sciences chemical sciences analytical chemistry mass spectrometry
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
B.P.105 TALENCE
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.