Objective
Shiga-toxigenic Escherichia coli (STEG) are highly adapted food-borne intestinal pathogens causing haemorrhagic colitis and the haemolytic uremie syndrome (HUS). STEG constitute a major problem in human food safety as cattle represent an important reservoir of STEC strains. Ability of STEG to adhere to. and subvert normal signalling pathways of, host cells is a key process in their pathogenesis. The objective of this project is to dissect the cross talk between STEC and epithelial cells on both the protein and genomic level. We will focus our study on STEC of serogroup O157 which are the most commonly associated with severe human disease. Host cellular targets of STEC translocated effector proteins will be identified using the yeast two-hybrid technique and their contribution to the integrated process of infection will be assessed. Using DNA micro array we will determine global alterations in bacterial gene expression in response to infection of eukaryotic cells. Available STEC specific oligo chips will allow us to monitor gene expression at different stages of infection and during adhesion to a variety of cell types. These data will deepen our knowledge of the molecular dialogue between STEC and their host cells and could lead to identification of new targets for therapeutics, prevention or controls strategies. Moreover, this project should allow me to considerably improve and complement my previous training in bacterial pathogenicity and will expand my knowledge into novel disciplines of genomics, as well others cutting edge techniques, which will be a strong added value for achievement of my future research career and goals. We also expect that my integration in Pr Frankel's group will lead to strengthen the scientific collaboration and technologies exchanges with my previous lab at the Vet school of Toulouse which also focuses its studies on molecular and cellular basis of E. coli pathogenicity.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- engineering and technology other engineering and technologies food technology food safety
- agricultural sciences animal and dairy science domestic animals animal husbandry
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
LONDON
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.