Objective
The role of the cytoplasm tyrosine kinas Sky (spleen tyrosine lamas) was, until recently, uniquely studied in haematopoietic cells. In these cells, Sky is involved in coupling activated immunoreceptors to downstream signalling events that mediate diverse cellular responses including proliferation and differentiation. We focus on the Sky expression in epithelial cells and its role as a tumour suppressor hi breast cancer cells. Breast cancer remains the most common malignancy in women. Loss of Sky expression is associated with the acquisition of a malignant (invasive/metastasis) breast tumour phenotype. Its role as a potential tumour suppressor gene has been strengthened not only in breast cancer but also in acute lymphoblastic leukemia.Our observations strongly indicates that Sky might directly affect mitosis and cytokines is. For the first time, we demonstrated that Sky is localised at the coatrooms, a key organelle for cell division. In order to evaluate the role of Skin the control of mitosis, variations in its expression and activity during the cell cycle will be quantified and its subcellularlocalisation will be analysed by time-lapse video microscopy. To unravel its tumour suppressor mechanism, the identification of the downstream signalling effectors of Sky in non-haematopoietic cells is essential and will be investigated using a phosphoproteomic approach. The absence of the Sky protein in breast cancer cells is reflected by the loss of its mina expression and suggests regulation at the transcriptional level. Analysis of the promoter activity by co-transfixion and reporter assays will indicate the important regulatory elements for putative activating and repressing transcription factors. Furthermore, the analysis of differential gene expression associated with the loss of Sky expression, can point out other genes involved in the acquisition of a malignant breast tumour phenotype. This innovating study on the #
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy
- medical and health sciences clinical medicine oncology breast cancer
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
PARIS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.