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BIOTECHNOLOGICAL APPLICATION OF NOVEL ENZYMES FROM METAGENOME LIBRARIES

Objective

The field of Biotechnology is blossoming. The continued need for isolation and characterisation of new biocatalysts to be used on \"chemically and biologically exotic\" reactions with organic compounds, is broadly appreciate. The major challenges to be met in this field, are discovering an describing the diversity of novel enzymatic processes on this planet, with a novel number of applications, for example in the prevention of cardiovascular and age-related eye diseases, diabetes and cancers and stabilisation of foods. In this way, many potential applications await the development of enzymes (biocatalysts) that can catalyse difficult reactions involving chemical transformations not encountered in the biological word. Where will these biocatalysts come from? We propose to use the Hugh genetic reservoir (met genome) of non-colourable organisms as a resource for biotechnological products and processes.

The general objective of the research to develop processes a:
(i) Screening of new types of alkanet hydroxyls, which would be soluble, requiring no additional electron transfer proteins for biocatalysts, possessing terminal hydroxylation activity. These enzymes are capable to oxidized alkenes - octane, hexane, cyclopean, pentane, as well as provide specific hydroxylation processes of a variety of chemicals;

(ii) Screening new lipases and esterase’s and asses their utility to catalysed two \"chemically and biologically exotic\" reactions, such as synthesis of esters of antioxidant vitamins (C, E and A) with fatty acids and enantiomeric resolution of optically active compounds. Both groups of biocatalysts provide potentially attractive technological tools, offering a \"green chemistry\" approach for the production of a variety of choral chemicals as basic building blocks for the synthesis of pharmaceuticals, agrochemicals, pesticides and insecticides.

Call for proposal

FP6-2002-MOBILITY-11
See other projects for this call

Coordinator

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Address
C/ Serrano 117
Madrid
Spain