Objective
Epithelial tumours are characterized by a loss of their polarized cellular architecture, which is correlated with less adhesive and more migratory properties of the tumour cell. Genetic analysis in Drosophila melanogaster led to the identification of the bazo oka, crumbs and discs large group of genes implicated in the generation of cell polarity. In addition, the components of the discs large group, the tumour suppressor proteins Dlg/Lgl/Scrib are involved in the regulation of cell proliferation. However, the f unction of these genes in mammals is poorly understood.
The proposed project aims to understand how the proteins of the crumbs and discs large group regulate cell adhesion in mammalian epithelial cells and how they contribute to cell polarity. Moreover, the studies are anticipated to identify the important linkage between the regulation of cell polarity and cell proliferation. The main approaches and methods for the research project is a wound healing migration assay and siRNA experiments. By using these as says, we will look at the biochemistry and cell biology of the de novo assembly of epithelial cell junctions. In particular, we will focus on the identification of the network of protein interactions between components of the bazooka, crumbs and discs large group and how these protein complexes contribute to cell junction formation and cell polarity. Furthermore, the role of Rho-family GTPases such as Rho, Rad and Cdc42 in the formation of cell adhesion will be assessed.
Finally, the proteins Dig, Lgl and S crib are considered to have a potential function in the control of cell proliferation. We will dissect the molecular events by which these proteins link cell polarity and cell proliferation by using a combination of siRNA, microinjection and live-cell imaging techniques. The results of the proposed project will have important implications for the field of epithelial cell biology and embryonic development.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences developmental biology
- natural sciences biological sciences cell biology cell polarity
- medical and health sciences clinical medicine oncology
- natural sciences physical sciences optics microscopy confocal microscopy
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
LONDON
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.