Objective
All plant cells, maintain a pH gradient across the vacuolar membrane throughout their life. An acidic vacuolar environment is necessary for activation of many vacuolar functions, while a neutral environment is required for most cytoplasmic functions. The mechanisms by which pH-homeostasis is achieved are still not clear. In petunia, as in all coloured flowers, the vacuole is the place of storage for the anthocyanin pigments.
Anthocyanins behave as pH indicators and allow one to recognize mutants by their blue flower colour. In this way we identified 7 distinct PH loci in petunia that control vacuolar pH in the flower petals. We recently isolated PH4 and PH3 and found that they encode transcription factors, which presumably control the activity of downstream genes and proteins. Interestingly, we found that AN1, AN2 and AN11, transcription activators of the anthocyanin pathway, are also involved in pH regulation. To understand the biochemical mechanisms by which pH is regulated, we isolated a pool of target genes/proteins controlled by these regulatory PH and AN genes by cDNA-AFLP and microarray analysis.
The project presented here aims to characterize the target genes of the PH regulators to unravel the biochemical pathway responsible for pH control in flower epidermal cells. The project involves a broad spectrum of techniques (molecular and cellular biology, genetics and cell physiology) and therefore constitutes an excellent, multidisciplinary and complete training program.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics
- engineering and technology materials engineering colors
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences basic medicine physiology
You need to log in or register to use this function
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
AMSTERDAM
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.