Objetivo The classical view of genomes as linear sequences has been replaced by a vision of nuclear organization that is both dynamic and complex, with chromosomes and genes non-randomly positioned in the nucleus. Process compartmentalization and spatial location of genes modulate the transcriptional output of the genomes. However, how the interplay between genome structure and gene regulation is established and maintained is still unclear. The aim of this project is to explore whether the genome 3D structure acts as an information source for modulating transcription in response to external stimuli. With a genuine interdisciplinary team effort, we will study the conformation of the genome at various integrated levels, from the nucleosome fiber to the distribution of chromosomes territories in the nuclear space. We will generate high-resolution 3D models of the spatial organization of the genomes of distinct eukaryotic cell types in interphase to identify differences in the chromatin landscape. We will follow the time course of structural changes in response to cues that affect gene expression either permanently or transiently. We will analyze the changes in genome structure during the stable trans-differentiation of immortalized B cells to macrophages and during the transient hormonal responses of differentiated cells. We plan to establish novel functional strategies, based on targeted and high-throughput reporter assays, to assess the relevance of the spatial environment on gene regulation. Using sophisticated modeling and computational approaches, we will combine high-resolution data from chromosome interactions, super-resolution images and omics information. Our long-term plan is to implement a 3D browser for the comprehensive mapping of chromatin properties and genomic features, to better understand how external signals are integrated at the genomic, epigenetic and structural level to orchestrate changes in gene expression that are cell specific and dynamic. Ámbito científico engineering and technologymaterials engineeringfibersnatural sciencesbiological sciencesgeneticschromosomesnatural sciencesbiological sciencesgeneticsgenomes Programa(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) ERC-2013-SyG - ERC Synergy Grant Convocatoria de propuestas ERC-2013-SyG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-SyG - Synergy grant Investigador principal de referencia Miguel Beato Del Rosal Prof. Institución de acogida FUNDACIO CENTRE DE REGULACIO GENOMICA Aportación de la UE € 12 272 645,00 Dirección CARRER DOCTOR AIGUADER 88 08003 Barcelona España Ver en el mapa Región Este Cataluña Barcelona Tipo de actividad Research Organisations Investigador principal Thomas Graf (Prof.) Contacto administrativo Mariana Morlans (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Beneficiarios (2) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo FUNDACIO CENTRE DE REGULACIO GENOMICA España Aportación de la UE € 12 272 645,00 Dirección CARRER DOCTOR AIGUADER 88 08003 Barcelona Ver en el mapa Región Este Cataluña Barcelona Tipo de actividad Research Organisations Investigador principal Thomas Graf (Prof.) Contacto administrativo Mariana Morlans (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos FUNDACIO PARC CIENTIFIC DE BARCELONA La participación finalizó España Aportación de la UE Sin datos Dirección BALDIRI I REIXAC 4 08028 Barcelona Ver en el mapa Región Este Cataluña Barcelona Contacto administrativo Marta Molina (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos
