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Sucrose Synthase as Cost-Effective Mediator of Glycosylation Reactions

Objective

Glycosyl transferases (GT) are extremely efficient biocatalysts that can be used for the synthesis of special carbohydrates and glycoconjugates. Famous examples of such products include human milk oligosaccharides that serve as prebiotics, and glycosides of flavonoids with improved stability and solubility. Unfortunately, the large-scale application of GT has been hampered by their low operational stability and by the high cost of their glycosyl donor. This project aims to solve both problems by a combination of enzyme and process engineering. On the one hand, the production of stable biocatalysts will be facilitated by the development of a suitable expression system, the design of optimized variants and the development of immobilized formulations. On the other hand, the production and recycling of nucleotide-activated sugars will be accomplished by exploiting the reaction of sucrose synthase (SuSy).

With the help of SuSy, UDP-glucose can be produced from sucrose as cheap and abundant substrate. Furthermore, other nucleotide sugars can be obtained when sucrose analogues are employed. These alternative substrates will be produced here with fructansucrase (FS), an enzyme that can couple fructose to various monosaccharides. However, the activity of both FS and SuSy towards sucrose analogues will have to be improved to become economically viable. In that way, the proposed concept can be developed into a generic procedure, in which the nucleotide moiety can be recycled to establish a constant supply of glycosyl donor. The three enzymatic steps will be optimized and integrated into an efficient process for the production of glycosylated compounds. The economical potential of this technology will be demonstrated by the scale-up of selected reactions at the industrial facilities available in our consortium. The participants in this project comprise 3 universities, 1 research institute and 4 SME with complementary skills and expertise.

Call for proposal

FP7-KBBE-2013-7-single-stage
See other projects for this call

Coordinator

UNIVERSITEIT GENT
Address
Sint Pietersnieuwstraat 25
9000 Gent
Belgium
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 1 229 682
Administrative Contact
Evelien Vandevelde (Ms.)

Participants (7)

TECHNISCHE UNIVERSITAET GRAZ
Austria
EU contribution
€ 837 300
Address
Rechbauerstrasse 12
8010 Graz
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Bernd Nidetzky (Prof.)
JULIUS-MAXIMILIANS-UNIVERSITAT WURZBURG
Germany
EU contribution
€ 735 708
Address
Sanderring 2
97070 Wuerzburg
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Christian Gloggengiesser (Mr.)
AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain
EU contribution
€ 333 650
Address
Calle Serrano 117
28006 Madrid
Activity type
Research Organisations
Administrative Contact
Maria Angeles López Vázquez (Ms.)
BIO-PRODICT BV
Netherlands
EU contribution
€ 470 364
Address
Nieuwe Marktstraat 54
6511 AA Nijmegen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Henk-Jan Joosten (Dr.)
ACIB GMBH
Austria
EU contribution
€ 582 163
Address
Krenngasse 37/2
8010 Graz
Activity type
Research Organisations
Administrative Contact
Julia Studencki (Mrs.)
C-LECTA GMBH
Germany
EU contribution
€ 451 100
Address
Perlickstrasse 5
04103 Leipzig
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Carsten Fietz (Dr.)
GALAB LABORATORIES GMBH
Germany
EU contribution
€ 449 675
Address
Am Schleusengraben 7
21029 Hamburg
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Jürgen Kuballa (Dr.)