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Contenido archivado el 2024-06-18

Using evolution to uncover the importance of alternative pre mRNA splicing

Objetivo

A major mystery of the genome age is why complex and simple organisms all have a similar number of genes. My specialism is in ‘Alternative Splicing’ which is the means by which different products can be made from a single gene by differential incorporation of regions of pre-mRNA. Alternative splicing is therefore a mechanism to increase gene complexity in complex organisms like man and in complex tissues like the brain. Alternative splicing has since been shown to be a pervasive mechanism crucial to normal health and development. For two decades labs studied alternative splicing on a gene-by-gene basis. In the last ten years genomewide data has increased the number of known alternative events, and recent developments have found that at least 80% of genes produce alternative mRNAs. The major questions in the field currently concern which of these alternative splices are important, and the problem remains of how to answer this using a huge amount of data. The key innovation of this application is to use an evolutionary approach to identify the important alternative splices using a unique high throughput RT-PCR approach. The proposed project will involve collaboration with biological experts in Newcastle studying brain, muscle and stem cells, with an expert on evolution in France (Dr. Philippe Fort) and with the genomics platform (for which I worked for three years) in Quebec, Canada.

Ámbito científico (EuroSciVoc)

Programa(s)

Tema(s)

Convocatoria de propuestas

FP7-PEOPLE-2013-CIG
Consulte otros proyectos de esta convocatoria

Régimen de financiación

MC-CIG - Support for training and career development of researcher (CIG)

Coordinador

UNIVERSITY OF NEWCASTLE UPON TYNE
Aportación de la UE
€ 100 000,00
Dirección
KINGS GATE
NE1 7RU Newcastle Upon Tyne
Reino Unido
Región
North East (England) Northumberland and Tyne and Wear Tyneside
Tipo de actividad
Higher or Secondary Education Establishments
Enlaces
Coste total
Sin datos