Objective
The rise of pathogenic bacteria that are resistant against a variety of antibiotic treatment emphasizes the need for novel active molecules. One promising novel class of active molecules is genetically encoded and ribosomally synthesized peptides, as they offer the potential to employ directed evolution approaches across a huge sequence space directly on an active substance in order to engineer novel activities and thus introduce a novel engineering principle into antibiotic development. However, in many cases the activity of the peptide molecule depends on substantial posttranslational modifications (PTMs) which give the peptides conformational stability and protect against proteolytic degradation. Consequently, the degrees of freedom in the engineering of such peptides on sequence level are highly dependent on the flexibility of the PTM-machinery to accept such changes. Here, we propose a synthetic biology approach to study systematically the flexibility of PTM-machineries for the class of lanthipepetides in order to assemble a highly flexible machinery that will support a broad range of engineering measures and thus optimize the chances to discover molecules with modified or completely novel activities. We also propose to implement a high-throughput screen for the bioactivity of these molecules in order to be able to engineer specific enzymes of the PTM-machinery for increased flexibility and to finally implement large scale directed evolution screens for novel antibiotic activities.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences synthetic biology
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2013-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
8092 Zuerich
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.