Objective
Fluorescent fusion tags have revolutionized many fields in molecular biology. Since the first applications of the Green Fluorescent Protein (GFP) extracted from the jellyfish Aequorea victoria, the photophysical properties of the protein have been improved and modified to optimize their application in various novel in vivo imaging techniques. These applications exploit, unique properties of fluorescence proteins (FPs), such as pH-sensitivity, and Förster resonant energy transfer (FRET), photoactivation, and photoconversion. The engineering of FPs has produced new variants emitting in the blue, cyan and yellow range of the spectrum and created a rich palette of promising new features. In particular the development of species emitting in the far red (RFP) has gained impetus by the discovery of new native FPs from corals (anthozoae). However, most of these newly designed FPs lack important characteristics of the GFP family such as brightness, photostability, and fluorescence at low pH. Therefore, a great need is perceived to better understand the photophysics of the GFP chromophore and its derivatives in the new FPs and how the protein environment controls it.
The goals of this project are (1) the validation of experimentally derived models for the photo-induced processes in FPs, (2) the systematic determination of the factors that control absorption/emission wavelength, absorptivity, uorescence quenching via internal conversion. In particular, the effects of electrostatic and steric interactions and hydrogen bonded networks between chromophore and binding pocket on the optical properties and excited-state dynamics will be investigated.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences molecular biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2013-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
48940 LEIOA
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.