Objective
The emergence of highly diverse resistance mechanisms among pathogens requires their detailed analysis to guarantee an efficient medical treatment. The gold standard in clinical diagnostics is based on the cultivation of bacteria and their phenotypical characterisation. However, these methods are labour-intensive and time-consuming lasting in some cases up to a few weeks. Thus, faster diagnostic techniques are necessary to ensure an immediate and targeted treatment of the patient. DNA-based diagnostics can provide the relevant results within a few hours.
The requirements for a clinical DNA-based characterisation method are high; more than 1000 clinically relevant antibiotic resistance genes, a few hundred phylogenetic marker genes and virulence factors have to be targeted (including SNP detection). The limit of detection has to be low because a few 100 bacterial cells in the blood system can lead to the death of the patient. It should be possible to analyse a wide range of clinical sample origins such as stool, blood, urine and saliva using the same test. In addition, the results have to be obtained within a single working day.
In our project, we will develop two diagnostic systems that can be with direct sample material from patients. Thus, the time-consuming cultivation of pathogens will be avoided. Additionally, the test will be more sensitive, specific and faster than any other test on the market using an innovative DNA probe concept.
Field of science
- /natural sciences/biological sciences/molecular biology
- /natural sciences/biological sciences/genetics and heredity/dna
- /natural sciences/biological sciences/microbiology/bacteriology
- /medical and health sciences/basic medicine/pharmacology and pharmacy/drug resistance/antibiotic resistance
Programme(s)
Call for proposal
H2020-PHC-2014-two-stage
See other projects for this call
Funding Scheme
RIA - Research and Innovation actionCoordinator
69622 Villeurbanne Cedex
France
Participants (9)
1210 Wien
CV4 8UW Coventry
10 000 Zagreb
6525 EZ Nijmegen
3500 Hasselt
69004 Lyon
28359 Bremen
LL55 2BD Caernarfon
69616 Villeurbanne
