An integrative approach to uncover the multilevel regulation of 20S proteasome degradation

Project Information

20SComplexity

Grant agreement ID: 636752

DOI

10.3030/636752

Project closed

EC signature date 16 March 2015

Start date 1 April 2015

End date 31 March 2020

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 500 000,00

EU contribution

€ 1 500 000,00

Coordinated by

WEIZMANN INSTITUTE OF SCIENCE
Israel

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (19)

Probing allosteric mechanisms using native mass spectrometry

Author(s): Michal Sharon, Amnon Horovitz
Published in: Current Opinion in Structural Biology, Issue 34, 2015, Page(s) 7-16, ISSN 0959-440X
Publisher: Elsevier BV
DOI: 10.1016/j.sbi.2015.05.002

Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis

Author(s): Miri Sokolovski, Jelena Cveticanin, Déborah Hayoun, Ilia Korobko, Michal Sharon, Amnon Horovitz
Published in: Nature Communications, Issue 8/1, 2017, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-017-00285-1

Post-translational regulation of p53 function through 20S proteasome-mediated cleavage

Author(s): Hilla Solomon, Bastian Bräuning, Irit Fainer, Gili Ben-Nissan, Stav Rabani, Naomi Goldfinger, Oren Moscovitz, Zippora Shakked, Varda Rotter, Michal Sharon
Published in: Cell Death and Differentiation, Issue 24/12, 2017, Page(s) 2187-2198, ISSN 1350-9047
Publisher: Nature Publishing Group
DOI: 10.1038/cdd.2017.139

The application of ion-mobility mass spectrometry for structure/function investigation of protein complexes

Author(s): Gili Ben-Nissan, Michal Sharon
Published in: Current Opinion in Chemical Biology, Issue 42, 2018, Page(s) 25-33, ISSN 1367-5931
Publisher: Elsevier BV
DOI: 10.1016/j.cbpa.2017.10.026

Exposing the subunit diversity and modularity of protein complexes by structural mass spectrometry approaches

Author(s): Dror S. Chorev, Gili Ben-Nissan, Michal Sharon
Published in: PROTEOMICS, Issue 15/16, 2015, Page(s) 2777-2791, ISSN 1615-9853
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/pmic.201400517

Native Mass Spectrometry of Recombinant Proteins from Crude Cell Lysates

Author(s): Jinrui Gan, Gili Ben-Nissan, Galina Arkind, Mark Tarnavsky, Devin Trudeau, Lianet Noda Garcia, Dan S. Tawfik, Michal Sharon
Published in: Analytical Chemistry, Issue 89/8, 2017, Page(s) 4398-4404, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.7b00398

Triple-Stage Mass Spectrometry Unravels the Heterogeneity of an Endogenous Protein Complex

Author(s): Gili Ben-Nissan, Mikhail E. Belov, David Morgenstern, Yishai Levin, Orly Dym, Galina Arkind, Carni Lipson, Alexander A. Makarov, Michal Sharon
Published in: Analytical Chemistry, Issue 89/8, 2017, Page(s) 4708-4715, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.7b00518

The Parkinson’s-associated protein DJ-1 regulates the 20S proteasome

Author(s): Oren Moscovitz, Gili Ben-Nissan, Irit Fainer, Dan Pollack, Limor Mizrachi, Michal Sharon
Published in: Nature Communications, Issue 6, 2015, Page(s) 6609, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/ncomms7609

CSNAP Is a Stoichiometric Subunit of the COP9 Signalosome

Author(s): Shelly Rozen, Maria G. Füzesi-Levi, Gili Ben-Nissan, Limor Mizrachi, Alexandra Gabashvili, Yishai Levin, Shifra Ben-Dor, Miriam Eisenstein, Michal Sharon
Published in: Cell Reports, Issue 13/3, 2015, Page(s) 585-598, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2015.09.021

Structural Characterization of Missense Mutations Using High Resolution Mass Spectrometry: A Case Study of the Parkinson’s-Related Protein, DJ-1

Author(s): Gili Ben-Nissan, Almog Chotiner, Mark Tarnavsky, Michal Sharon
Published in: Journal of The American Society for Mass Spectrometry, Issue 27/6, 2016, Page(s) 1062-1070, ISSN 1044-0305
Publisher: Elsevier BV
DOI: 10.1007/s13361-016-1379-z

Tuning the proteasome to brighten the end of the journey

Author(s): Thibault Mayor, Michal Sharon, Michael H. Glickman
Published in: Cell Physiology, 2016, ISSN 0363-6143
Publisher: American Physiological Society
DOI: 10.1152/ajpcell.00198.2016

Mass spectrometry: a technique of many faces

Author(s): Maya A. Olshina, Michal Sharon
Published in: Quarterly Reviews of Biophysics, 2016, ISSN 0033-5835
Publisher: Cambridge University Press
DOI: 10.1017/S0033583516000160

Estimating Interprotein Pairwise Interaction Energies in Cell Lysates from a Single Native Mass Spectrum

Author(s): Jelena Cveticanin, Ravit Netzer, Galina Arkind, Sarel J. Fleishman, Amnon Horovitz, Michal Sharon
Published in: Analytical Chemistry, Issue 90/17, 2018, Page(s) 10090-10094, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.8b02349

Rapid characterization of secreted recombinant proteins by native mass spectrometry

Author(s): Gili Ben-Nissan, Shay Vimer, Shira Warszawski, Aliza Katz, Meital Yona, Tamar Unger, Yoav Peleg, David Morgenstern, Hadas Cohen-Dvashi, Ron Diskin, Sarel J. Fleishman, Michal Sharon
Published in: Communications Biology, Issue 1/1, 2018, ISSN 2399-3642
Publisher: Nature
DOI: 10.1038/s42003-018-0231-3

Conformational states during vinculin unlocking differentially regulate focal adhesion properties

Author(s): Dror S. Chorev, Tova Volberg, Ariel Livne, Miriam Eisenstein, Bruno Martins, Zvi Kam, Brigitte M. Jockusch, Ohad Medalia, Michal Sharon, Benny Geiger
Published in: Scientific Reports, Issue 8/1, 2018, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-018-21006-8

The Contribution of the 20S Proteasome to Proteostasis

Author(s): Fanindra Kumar Deshmukh, Dana Yaffe, Maya A. Olshina, Gili Ben-Nissan, Michal Sharon
Published in: Biomolecules, Issue 9/5, 2019, Page(s) 190, ISSN 2218-273X
Publisher: MDPI
DOI: 10.3390/biom9050190

Direct characterization of overproduced proteins by native mass spectrometry

Author(s): Shay Vimer, Gili Ben-Nissan, Michal Sharon
Published in: Nature Protocols, Issue 15/2, 2020, Page(s) 236-265, ISSN 1754-2189
Publisher: Nature Publishing Group
DOI: 10.1038/s41596-019-0233-8

Regulation of the 20S Proteasome by a Novel Family of Inhibitory Proteins

Author(s): Maya A. Olshina, Galina Arkind, Fanindra Kumar Deshmukh, Irit Fainer, Mark Taranavsky, Daniel Hayat, Shifra Ben-Dor, Gili Ben-Nissan, Michal Sharon
Published in: Antioxidants & Redox Signaling, Issue 32/9, 2020, Page(s) 636-655, ISSN 1523-0864
Publisher: Mary Ann Liebert Inc.
DOI: 10.1089/ars.2019.7816

CSNAP, the smallest CSN subunit, modulates proteostasis through cullin-RING ubiquitin ligases

Author(s): Maria G. Füzesi-Levi, Irit Fainer, Radoslav Ivanov Enchev, Gili Ben-Nissan, Yishai Levin, Meital Kupervaser, Gilgi Friedlander, Tomer Meir Salame, Reinat Nevo, Matthias Peter, Michal Sharon
Published in: Cell Death & Differentiation, Issue 27/3, 2020, Page(s) 984-998, ISSN 1350-9047
Publisher: Nature Publishing Group
DOI: 10.1038/s41418-019-0392-8

Book chapters (1)

Structural mass spectrometry approaches to study the 20S proteasome

Author(s): Gili Ben-Nissan, Shay Vimer, Mark Tarnavsky, Michal Sharon
Published in: Ubiquitin-dependent Protein Degradation, Issue 619, 2019, Page(s) 179-223, ISBN 9780-128186671
Publisher: Elsevier
DOI: 10.1016/bs.mie.2018.12.029