Periodic Reporting for period 4 - BacBio (Mechanistic and functional studies of Bacillus biofilms assembly on plants, and their impact in sustainable agriculture and food safety)
Reporting period: 2019-09-01 to 2021-02-28
In order to propose feasible strategies oriented to promote biofilm formation and therefore a positive effect of beneficial bacteria, and at the same time, banner or impede the establishment of the pathogenic, it is important to know the bacterial factors involved in such developmental program and look for specific targets.
In our project we are interested in the following specific aims:
1.- To understand the process of amyloidogenesis. To cover this study, we propose to move alternatively from in vitro conditions to plants, using a variety of techniques, including chemistry, biophysics or cell biology.
2.- To know how the plant modulates the formation fo biofilms, with special emphasis in the effect on amyloids, and reciprocally, which is the response of the plant to the establishment of such communities.
3.- To investigate how the two bacterial species communicate with each other or even other species that may co-exist in the same habitat.
2. We have also found a diversification of functions of the ECM in the interaction with the environment. This interaction is chemical (signaling) or structural, and permits the establishment of stable bacterial communities on plants, or a beneficial inter-kingdom communication.
3. We have discovered which appears to being the main function of the B. subtilis amyloid, to provide integrity to the cell membrane when cells enter in stationary phase of growth, besides their role in biofilm assembly. These two functions affect the persistence and antagonistic interaction with fungal pathogens.
5. Different plant surfaces possess distinctive morphological and chemical features that modulate the ecology of Bacilli and the expression of the genes related to biofilm.
6. The ability of B. cereus to induce injury in intestine, is alternatively based on vegetative cells are able to persist on vegetables. This is possible to the maintenance of other ecological skills as biofilm formation.
These findings represent a breakthrough on the real implication of bacterial biofilms in the ecology of bacterial in plants, and how they can be handled to improve the beneficial contribution to the health of the crops. Thus, from an applied perspective, our findings can be easily translated to the improvement in the implementation of microbes and derived molecules in the frame of sustainable programs of production of crop protection. The study on B. cereus has revealed that beyond spores and toxins it is important to pay attention to vegetative cells which can cause a plethora of symptoms in humans, from diarrhea or vomit to death, and therefore make necessary to revise the procedure used for the detection.
These findings has been disseminated in the research community in the form of research manuscripts, or presentation in seminars and conferences. In the other hand, we have contributed also to their dissemination to the general public by participating in different diffusion media. Some examples are:
Participation in the European researcher' night (2018-2019). https://lanochedelosinvestigadores.fundaciondescubre.es/investigador/diego-f-romero-hinojosa/
Diario sur: https://www.bacbiolab.com/wp-content/uploads/2020/11/cronica-universitaria-27-10-2020-1.pdf
Programa tesis: http://www.canalsur.es/television/programas/tesis/detalle/62.html?video=1443163&sec=
Social media: tweeter: diegoromerohi
We have been able to validate the knowledge accumulated in in vitro biofilm studies to plants, decisively contributing to the fundamentals of microbial ecological. It is clear that bacteria utilizes a variety of factors to live and communicate with plants, and we have expand our knowledge on this aspect. In addition, our research is also demonstrating how relevant is the plant host not only immunologically, but also metabolically and structurally (topology). As humans, the plants posses an immunological system that respond to external aggressions or changes, one of them the bacterial communities that live in association with. The final outcome, disease development, or pathogen establishment, will be the result of a complex network of interactions, and dialogues between all the players: plant pathogens, beneficial, human pathogens. The combination, again of disciplines as microbiology, plant physiology with cutting-edge analytical chemistry techniques and diverse OMICs have decisively contributed to success in these studies and provide interesting conclusion and novel hypothesis. In all these studies we are moving from bacterial population behavior, to really visualize the existence of changes, to further embark in more detailed and specific studies at cellular or individual levels.
In summary our project has offer responses to basic questions on microbial cell biology and physiology, to really understand in a more holistic perspective how they do coordinate to generate a response in the context of the interaction with plants, a knowledge that would be granted to: 1) the development of more robust and feasible biological control strategies oriented to reduce the use of chemicals either alone or in combination with other management strategies in the context of sustainable practices and 2) contribute to eradicate or impede the establishment of human pathogen communities in ready to eat vegetable or fruits and responsible of human diseases.