Project description DEENESFRITPL Identification of critical transcription factors regulating strawberry ripening Fruit breeding programmes often prioritise agronomic traits while overlooking the crucial role that ripening plays in the development of flavour quality. This results in a limited genetic variability in various fleshy fruits including strawberries, as current cultivars are derived from a narrow germplasm stock. Unlike climacteric fruits, strawberries undergo a non-climacteric ripening process that is not influenced by the ethylene hormone. To address these issues, the EU-funded TRANSFR-Q project will use Fragaria vesca, a wild relative of the cultivated strawberry, as a model organism. The project aims to identify the key transcription factors that regulate fruit ripening, validate their role and elucidate the gene regulatory networks that control the ripening process. Show the project objective Hide the project objective Objective Ripening is the critical step for the development of flavour quality in fruit. This character has significantly declined in many fleshy fruits over recent decades, primarily due to the focus of current breeding programs on agronomic traits such as production, firmness, and postharvest shelf life. This strategy has caused a tunnelling effect on genetic variability in many crops. This is particularly significant in strawberry, where current cultivars are derived from a narrow germplasm stock. A notable feature of strawberry fruit is that undergoes a non-climacteric ripening program independent of the hormone ethylene, in contrast to well studied climacteric fruits such as tomato, where ethylene plays a central role. Therefore, improving fruit flavour in present strawberry varieties requires two important breakthroughs: 1) a precise understanding of non-climacteric fruit ripening regulation that will allow the targeting of relevant quality genes, and 2) the identification of unexploited allelic variants from wild germplasm to be introgressed through the generation of novel breeding lines. The first objective will be achieved by (i) focusing on the sequenced, diploid Fragaria vesca, a wild relative of the cultivated strawberry that will serve as a model, (ii) identifying key transcription factors (TFs) regulating fruit ripening by generating a stage- and tissue-specific gene expression map, (iii) using a candidate gene approach and reverse genetics based on gene silencing and TILLING to verify the role of these TFs, and (iv) defining the gene regulatory networks controlling the ripening process via integration of transcriptomic, metabolomic and ChIP-Seq data obtained from the stably silenced and/or TILLED lines. Finally, TRANSFR-Q plans to use this knowledge, combined with the identification of novel alleles from a core collection of Fragaria germplasm, to transfer flavour quality characters into current strawberry cultivars. Fields of science medical and health sciencesmedical biotechnologygenetic engineeringgene therapyagricultural sciencesagriculture, forestry, and fisheriesagriculturehorticulturefruit growingnatural sciencesmathematicspure mathematicsmathematical analysisfunctional analysisnatural scienceschemical sciencesorganic chemistryaliphatic compoundsnatural sciencesbiological sciencesgeneticsgenomes Programme(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Topic(s) ERC-StG-2014 - ERC Starting Grant Call for proposal ERC-2014-STG See other projects for this call Funding Scheme ERC-STG - Starting Grant Coordinator UNIVERSIDAD DE MALAGA Net EU contribution € 1 500 000,00 Address Avda cervantes, num. 2 29016 Malaga Spain See on map Region Sur Andalucía Málaga Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 Beneficiaries (1) Sort alphabetically Sort by Net EU contribution Expand all Collapse all UNIVERSIDAD DE MALAGA Spain Net EU contribution € 1 500 000,00 Address Avda cervantes, num. 2 29016 Malaga See on map Region Sur Andalucía Málaga Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00
