Quantitative Nanoscale Visualization of Macromolecular Complexes in Live Cells using Genetic Code Expansion and High-Resolution Imaging

Project Information

VisuLive

Grant agreement ID: 639313

DOI

10.3030/639313

Project closed

EC signature date 31 March 2015

Start date 1 April 2015

End date 31 March 2021

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 625 000,00

EU contribution

€ 1 625 000,00

Coordinated by

BEN-GURION UNIVERSITY OF THE NEGEV
Israel

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Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (7)

Live Cell Imaging of Bioorthogonally Labelled Proteins Generated With a Single Pyrrolysine tRNA Gene

Author(s): Noa Aloush, Tomer Schvartz, Andres I. König, Sarit Cohen, Eugene Brozgol, Benjamin Tam, Dikla Nachmias, Oshrit Ben-David, Yuval Garini, Natalie Elia, Eyal Arbely
Published in: Scientific Reports, Issue 8/1, 2018, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-018-32824-1

A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag

Author(s): Inbar Segal, Dikla Nachmias, Andres Konig, Ariel Alon, Eyal Arbely, Natalie Elia
Published in: BMC Biology, Issue 18/1, 2020, ISSN 1741-7007
Publisher: BioMed Central
DOI: 10.1186/s12915-019-0708-7

Live cell single molecule tracking and localization microscopy of bioorthogonally labeled plasma membrane proteins

Author(s): Andres I. König, Raya Sorkin, Ariel Alon, Dikla Nachmias, Kalyan Dhara, Guy Brand, Ofer Yifrach, Eyal Arbely, Yael Roichman, Natalie Elia
Published in: Nanoscale, Issue 12/5, 2020, Page(s) 3236-3248, ISSN 2040-3364
Publisher: Royal Society of Chemistry
DOI: 10.1039/c9nr08594g

Direct fluorescent-dye labeling of α-tubulin in mammalian cells for live cell and superresolution imaging

Author(s): Tomer Schvartz, Noa Aloush, Inna Goliand, Inbar Segal, Dikla Nachmias, Eyal Arbely, Natalie Elia
Published in: Molecular Biology of the Cell, Issue 28/21, 2017, Page(s) 2747-2756, ISSN 1059-1524
Publisher: American Society for Cell Biology
DOI: 10.1091/mbc.E17-03-0161

Single-Plasmid-Based System for Efficient Noncanonical Amino Acid Mutagenesis in Cultured Mammalian Cells

Author(s): Sarit Cohen, Eyal Arbely
Published in: ChemBioChem, Issue 17/11, 2016, Page(s) 1008-1011, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201500681

Using unnatural amino acids to selectively label proteins for cellular imaging: a cell biologist viewpoint

Author(s): Natalie Elia
Published in: The FEBS Journal, Issue 288/4, 2021, Page(s) 1107-1117, ISSN 1742-464X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/febs.15477

Translational read-through promotes aggregation and shapes stop codon identity

Author(s): Lior Kramarski, Eyal Arbely
Published in: Nucleic Acids Research, Issue 48/7, 2020, Page(s) 3747-3760, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkaa136