Click Chemistry for Future Gene Therapies to Benefit Citizens, Researchers and Industry

Project Information

ClickGene

Grant agreement ID: 642023

DOI 10.3030/642023

Project closed

EC signature date 5 December 2014

Start date 1 January 2015

End date 31 December 2018

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost € 3 556 990,44

EU contribution € 3 556 990,44

Coordinated by

DUBLIN CITY UNIVERSITY
Ireland

Deliverables

Other (2)

Summer School “Enzymatic Chemical Nucleases”

Summer school providing hands-on training and focused teaching with input from ESRs.

SCNAC conference in Ceske Krumlov 2018

ESRs will present their most significant findings at this renowned conference on “Chemistry of Nucleic Acid Components”

Websites, patent fillings, videos etc. (4)

 FAQ sessions in schools

Each ESR will contact a local high school and participate in a chemistry lesson in the final grade to present the concept of “click chemistry” and “gene editing” and to answer FAQs of the pupils

Website

The ClickGene website will address all key user groups on its public pages: i.) general public; ii.) other researchers; and iii.) potential future employers from industry.

Video

A Video illustrating the process of “click chemistry” and key actors in this field of research, will be produced by ESRs during one of ClcikGene Meetings and uploaded to YouTube.

Social media

ClickGene accounts will be opened by the ESRs on ResearchGate and in other online communities to facilitate chatting and blogging on hot topics around click chemistry.

Publications

Peer reviewed articles (24)

5-Formylcytosine Could Be a Semipermanent Base in Specific Genome Sites

Author(s): Meng Su, Angie Kirchner, Samuele Stazzoni, Markus Müller, Mirko Wagner, Arne Schröder, Thomas Carell
Published in: Angewandte Chemie International Edition, Issue 55/39, 2016, Page(s) 11797-11800, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201605994

5-Substituted Pyrimidine and 7-Substituted 7-Deazapurine dNTPs as Substrates for DNA Polymerases in Competitive Primer Extension in the Presence of Natural dNTPs

Author(s): Hana Cahová, Alessandro Panattoni, Pavel Kielkowski, Jindřich Fanfrlík, Michal Hocek
Published in: ACS Chemical Biology, Issue 11/11, 2016, Page(s) 3165-3171, ISSN 1554-8929
Publisher: American Chemical Society
DOI: 10.1021/acschembio.6b00714

Solvatochromic fluorene-linked nucleoside and DNA as color-changing fluorescent probes for sensing interactions

Author(s): Dmytro Dziuba, Petr Pospíšil, Ján Matyašovský, Jiří Brynda, Dana Nachtigallová, Lubomír Rulíšek, Radek Pohl, Martin Hof, Michal Hocek
Published in: Chem. Sci., Issue 7/9, 2016, Page(s) 5775-5785, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/C6SC02548J

2-Substituted dATP Derivatives as Building Blocks for Polymerase-Catalyzed Synthesis of DNA Modified in the Minor Groove

Author(s): Ján Matyašovský, Pavla Perlíková, Vincent Malnuit, Radek Pohl, Michal Hocek
Published in: Angewandte Chemie International Edition, Issue 55/51, 2016, Page(s) 15856-15859, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201609007

Fatty Acids in Membranes as Homeostatic, Metabolic and Nutritional Biomarkers: Recent Advancements in Analytics and Diagnostics

Author(s): Carla Ferreri, Annalisa Masi, Anna Sansone, Giorgia Giacometti, Anna Larocca, Georgia Menounou, Roberta Scanferlato, Silvia Tortorella, Domenico Rota, Marco Conti, Simone Deplano, Maria Louka, Anna Maranini, Arianna Salati, Valentina Sunda, Chryssostomos Chatgilialoglu
Published in: Diagnostics, Issue 7/1, 2017, Page(s) 1, ISSN 2075-4418
Publisher: MDPI AG
DOI: 10.3390/diagnostics7010001

Flexible Alkyne-Linked Thymidine Phosphoramidites and Triphosphates for Chemical or Polymerase Synthesis and Fast Postsynthetic DNA Functionalization through Copper-Catalyzed Alkyne–Azide 1,3-Dipolar Cycloaddition

Author(s): Alessandro Panattoni, Radek Pohl, Michal Hocek
Published in: Organic Letters, Issue 20/13, 2018, Page(s) 3962-3965, ISSN 1523-7060
Publisher: American Chemical Society
DOI: 10.1021/acs.orglett.8b01533

Polypyridyl-Based Copper Phenanthrene Complexes: A New Type of Stabilized Artificial Chemical Nuclease

Author(s): Nicoló Zuin Fantoni, Zara Molphy, Creina Slator, Georgia Menounou, Gianluca Toniolo, George Mitrikas, Vickie McKee, Chryssostomos Chatgilialoglu, Andrew Kellett
Published in: Chemistry - A European Journal, Issue 25/1, 2019, Page(s) 221-237, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201804084

[Cu(TPMA)(Phen)](ClO 4 ) 2 : Metallodrug Nanocontainer Delivery and Membrane Lipidomics of a Neuroblastoma Cell Line Coupled with a Liposome Biomimetic Model Focusing on Fatty Acid Reactivity

Author(s): Gianluca Toniolo, Maria Louka, Georgia Menounou, Nicolò Zuin Fantoni, George Mitrikas, Eleni K. Efthimiadou, Annalisa Masi, Massimo Bortolotti, Letizia Polito, Andrea Bolognesi, Andrew Kellett, Carla Ferreri, Chryssostomos Chatgilialoglu
Published in: ACS Omega, Issue 3/11, 2018, Page(s) 15952-15965, ISSN 2470-1343
Publisher: ACS Publications
DOI: 10.1021/acsomega.8b02526

Fluorogenic thiazole orange TOTFO probes stabilise parallel DNA triplexes at pH 7 and above

Author(s): Sarah Walsh, Afaf Helmy El-Sagheer, Tom Brown
Published in: Chemical Science, Issue 9/39, 2018, Page(s) 7681-7687, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8sc02418a

2-Allyl- and Propargylamino-dATPs for Site-Specific Enzymatic Introduction of a Single Modification in the Minor Groove of DNA

Author(s): Ján Matyašovský, Radek Pohl, Michal Hocek
Published in: Chemistry - A European Journal, Issue 24/56, 2018, Page(s) 14938-14941, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201803973

Development of multi-layered and multi-sensitive polymeric nanocontainers for cancer therapy: in vitro evaluation

Author(s): Gianluca Toniolo, Eleni K. Efthimiadou, George Kordas, Chryssostomos Chatgilialoglu
Published in: Scientific Reports, Issue 8/1, 2018, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-018-32890-5

Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

Author(s): Ilenia Manuguerra, Stefano Croce, Afaf H. El-Sagheer, Abhichart Krissanaprasit, Tom Brown, Kurt V. Gothelf, Antonio Manetto
Published in: Chemical Communications, Issue 54/36, 2018, Page(s) 4529-4532, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8cc00738a

High predictive values of RBC membrane-based diagnostics by biophotonics in an integrated approach for Autism Spectrum Disorders

Author(s): Giorgia Giacometti, Carla Ferreri, Anna Sansone, Chryssostomos Chatgilialoglu, Carla Marzetti, Ellas Spyratou, Alexandros G. Georgakilas, Marina Marini, Provvidenza M. Abruzzo, Alessandra Bolotta, Alessandro Ghezzo, Renato Minguzzi, Annio Posar, Paola Visconti
Published in: Scientific Reports, Issue 7/1, 2017, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-017-10361-7

Altered Red Blood Cell Membrane Fatty Acid Profile in Cancer Patients

Author(s): Javier Amézaga, Sara Arranz, Ander Urruticoechea, Gurutze Ugartemendia, Aitziber Larraioz, Maria Louka, Matxalen Uriarte, Carla Ferreri, Itziar Tueros
Published in: Nutrients, Issue 10/12, 2018, Page(s) 1853, ISSN 2072-6643
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/nu10121853

trans-Double Bond-Containing Liposomes as Potential Carriers for Drug Delivery

Author(s): Giorgia Giacometti, Marina Marini, Kyriakos Papadopoulos, Carla Ferreri, Chryssostomos Chatgilialoglu
Published in: Molecules, Issue 22/12, 2017, Page(s) 2082, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules22122082

Fatty acid-based lipidomics and membrane remodeling induced by apoE3 and apoE4 in human neuroblastoma cells

Author(s): Paraskevi Prasinou, Ioannis Dafnis, Giorgia Giacometti, Carla Ferreri, Angeliki Chroni, Chryssostomos Chatgilialoglu
Published in: Biochimica et Biophysica Acta (BBA) - Biomembranes, Issue 1859/10, 2017, Page(s) 1967-1973, ISSN 0005-2736
Publisher: Elsevier BV
DOI: 10.1016/j.bbamem.2017.07.001

Synthesis of RNA Containing 5-Hydroxymethyl-, 5-Formyl-, and 5-Carboxycytidine

Author(s): Iacovos N. Michaelides, Nobuhiro Tago, Bastien Viverge, Thomas Carell
Published in: Chemistry - A European Journal, Issue 23/63, 2017, Page(s) 15894-15898, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201704216

Dendrimer-based signal amplification of click-labelled DNA in situ

Author(s): Raddaoui, Nada; Stazzoni, Samuele; Möckl, Leonhard; Viverge, Bastien; Geiger, Florian; Engelke, Hanna; Bräuchle, Christoph; Carell, Thomas
Published in: Chembiochem, Issue 18/17, 2017, Page(s) 1716-1720, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.5282/ubm/epub.60576

5-Formylcytosin ist vermutlich eine semi-permanente Base an definierten Genompositionen

Author(s): Su, Meng ; Kirchner, Angie ; Stazzoni, Samuele; Müller, Markus ; Wagner, Mirko; Schröder, Arne; Carell, Thomas
Published in: Angewandte Chemie, Issue 128/39, 2016, Page(s) 11974-11978, ISSN 1521-3757
Publisher: Wiley Online Library
DOI: 10.5282/ubm/epub.60565

A Click-Chemistry Linked 2’3’-cGAMP Analog

Author(s): Dialer, Clemens Reto ; Stazzoni, Samuele; Drexler, David Jan; Müller, Felix Moritz; Veth, Simon; Pichler, Alexander; Okamura, Hidenori ; Witte, Gregor ; Hopfner, Karl-Peter ; Carell, Thomas
Published in: Chemistry-A European Journal, Issue 25, 2018, Page(s) 2089-2095, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.5282/ubm/epub.60579

2-Substituted dATP Derivatives as Building Blocks for Polymerase-Catalyzed Synthesis of DNA Modified in the Minor Groove

Author(s): Ján Matyašovský, Pavla Perlíková, Vincent Malnuit, Radek Pohl, Michal Hocek
Published in: Angewandte Chemie, Issue 128/51, 2016, Page(s) 16088–16091, ISSN 1521-3757
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/ange.201609007

Genome Engineering with Synthetic Copper Nucleases

Author(s): A. Kellett, N. Fantoni, T. Lauria
Published in: Synlett, Issue 26/19, 2015, Page(s) 2623-2626, ISSN 0936-5214
Publisher: Georg Thieme Verlag
DOI: 10.1055/s-0035-1560709

Building up spectral libraries for mapping erythrocytes by hyperspectral dark field microscopy

Author(s): Marco Conti, Roberta Scanferlato, Maria Louka, Anna Sansone, Carla Marzetti, Carla Ferreri
Published in: Biomedical Spectroscopy and Imaging, Issue 5/2, 2016, Page(s) 175-184, ISSN 2212-8808
Publisher: IOS Press
DOI: 10.3233/BSI-160133

Hexadecenoic Fatty Acid Isomers in Human Blood Lipids and Their Relevance for the Interpretation of Lipidomic Profiles

Author(s): Anna Sansone, Evanthia Tolika, Maria Louka, Valentina Sunda, Simone Deplano, Michele Melchiorre, Dimitrios Anagnostopoulos, Chryssostomos Chatgilialoglu, Cesare Formisano, Rosa Di Micco, Maria Rosaria Faraone Mennella, Carla Ferreri
Published in: PLOS ONE, Issue 11/4, 2016, Page(s) e0152378, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0152378