Deliverables
A subset of cultivars, common to all collections will be considered for the following aspects: tree shape and behavior, variation on oil acidity composition and polyphenols content, abiotic stress tolerance and fruit production.
All information available on the potential to grow under drought stress conditions will be organized into the common database.
All information available on the potential to grow under salt stress conditions will be organized into the common database.
A common protocol to collect morphological parameters will be established. Aspects to consider include the number and type of traits, phenology, representative samples, imaging technology, softwares for data elaboration and data output.
Main traits that will be explored include: oil quantity, polyphenols content, acidity composition, tree habit, fruit size, pulp/stone ratio, harvesting season, drought and cold tolerance, diseases (Spilocaea oleagina, Pseudomonas savastanoi, Verticillium dahliae, etc). Markers linked to these traits will be searched by the approach of association mapping.
All information available on tree vigor and canopy shape will be organized into the common database.
Data made available on inter-incompatibility of cultivars will be organized into the common database.
A new set of chloroplast markers will be developed starting from the set already published, including those able to discriminate among wild plants and related subspecies. A new set of cytoplasmic markers will be also made available from the whole mitochondrial genome sequence.
Data available on oil fatty acid composition will be collected for each cultivar and will be compared among the main collections considering other aspects such as: area of diffusion, altitude, latitude and climatic conditions, etc.
All information available on the potential to grow under heat stress conditions will be organized into the common database.
LRU will Identify the legal and ethical problems linked of the construction of the databank. In particular the problems as follows will be taken into consideration: - Problems concerning the property and the other rights relating the databank and the genetic material; - Problems related to the access rights.
A selection based on their discrimination power of poly-nucleotide SSR as tri-, tetra- and penta-nucleotide repeats will be done, from the whole genome sequence and from the numerous RNA libraries made available by partners
All information available on the best association of cultivars related to climatic, soil and biotic/abiotic stress resistance for the most relevant olive producing areas will be organized into the common database.
Updated version at the end of the project
All information available on early bearing will be organized into the common database.
A list of the most relevant varieties contributing to the olive oil production for the Mediterranean countries and new areas of olive cultivation of South (Argentina and Chile) and North America (Mexico) will be provided. Reference information will derive from International, National and Regional Olive Varietal collections previously characterized by molecular and morphological markers.
Markers linked to traits of agronomical, morphological or biological interest will also be searched in order to assign a specific trait to a relative marker
Data made available on self-incompatibility of cultivars will be organized into the common database.
Data available for each cultivar on susceptibility to olive leaf spot will be compared among the main collections considering other aspects such as: area of diffusion, climatic conditions, pathogen strain, etc.
The best SNP (Single Nucleotide Polymorphisms) markers, developed by the partners of the project by using Next Generation Sequencing (NGS) approach, will be selected to establish a common set of markers for cultivar discrimination and to build a SNP array.
An awareness and dissemination plan will be elaborated and will be updated and deployed along the project life and beyond. The different versions of the Dissemination Plan will include the activities that have actually been developed in the project in the previous plan and those foreseen for the next period. The Dissemination Plan will contain the following elements: 1) Identification and classification of target stakeholders to be addressed. 2) The dissemination methods and their specific associated activities. 3) Schedule and complementarily of the dissemination activities among partners. 4) The conditions to ensure proper dissemination of the generated knowledge. As was previously commented, the identification and communication with stakeholders is of main importance. The first Awareness and Dissemination Plan will include a list of stakeholders and the plan to address those, a close contact must be established, offering information in different formats in order to ensure it adequacy to the needs of each group of interest. This kind of contact will start early in the life of the project
Data available for each cultivar on susceptibility to olive fruit fly will be compared among the main collections considering other aspects such as: insect diffusion, number of generations, meteorological parameters, level of damage, etc.
The same action, previously described, will include also wild plants and related subspecies discriminated by a specific marker set.
Data available for each cultivar on susceptibility to Verticillium wilt will be compared among the main collections considering other aspects such as: area of diffusion, irrigation, tree damages, etc.
Data on oil composition in biophenols, secoiridoids, steroids and tocoferols will be reported for each cultivar on a common database.
A second and final release of the economical and institutional aspects at the end of the analysis.
From the analysis of 200 main cultivars with the set of markers selected within this project it will be possible to obtain a unique and stable profile for each variety, accepted as reference profile at world level.
All other information available on the varietal use, such as table olive varieties, oil characteristics, varieties showing strong adaptation to extreme conditions, will be organized into the common database..
Seminars and Workshops will be organized in correspondence of each deliverable.
A common database including all cultivar profiles will be built, able to compare olive varieties for their identification and discrimination.
Seminars and Workshops will be organized at the end of each secondment
The final Database version including the molecular profiles of cultivars, wild plants and related subspecies will be definitively published.
Project website “BeFOre” project will have its own web page, developed and maintained by ISAFOM. It will be used for the internal communication, and it will also form the basis for the administrative and the external communication. A book reporting the main results will be published on the web site of the project; Internal information will be restricted to partners, which have different rights for viewing parts of the information structure. The web site will also be used as a basic tool for external communications. In the public area we will develop a communication strategy towards the general public and selected target group. Here we will collect all material, which should be publicly accessible (presentations, papers, information material, posters, etc).
Based on the markers developed, a common platform will be established to discriminate among olive cultivars, wild plants and related subspecies in each lab involved in the project.
The final release of a common database will be done as an online free access version and as publications.
Publications
Author(s): Konstantinos N. Blazakis, Maria Kosma, George Kostelenos, Luciana Baldoni, Marina Bufacchi, Panagiotis Kalaitzis
Published in: Plant Methods, 13/1, 2017, ISSN 1746-4811
Publisher: BioMed Central
DOI: 10.1186/s13007-017-0261-8
Author(s): Ibrahim Hmmam, Roberto Mariotti, Benedetto Ruperti, Nicolò Cultrera, Luciana Baldoni, Gianni Barcaccia
Published in: Genetic Resources and Crop Evolution, 65/6, 2018, Page(s) 1733-1750, ISSN 0925-9864
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10722-018-0650-5
Author(s): R. Mariotti, N. G. M. Cultrera, S. Mousavi, F. Baglivo, M. Rossi, E. Albertini, F. Alagna, F. Carbone, G. Perrotta, Luciana Baldoni
Published in: Tree Genetics & Genomes, 12/6, 2016, ISSN 1614-2942
Publisher: Springer Verlag
DOI: 10.1007/s11295-016-1077-9
Author(s): Lorenzo León, Raúl de la Rosa, Leonardo Velasco, Angjelina Belaj
Published in: Frontiers in Plant Science, 9, 2018, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2018.00232
Author(s): Soraya Mousavi, Roberto Mariotti, Francesca Bagnoli, Lorenzo Costantini, Nicolò G. M. Cultrera, Kazem Arzani, Saverio Pandolfi, Giovanni Giuseppe Vendramin, Bahareh Torkzaban, Mehdi Hosseini-Mazinani, Luciana Baldoni
Published in: Annals of Botany, 119/8, 2017, Page(s) 1305-1318, ISSN 0305-7364
Publisher: Oxford University Press
DOI: 10.1093/aob/mcx027
Author(s): Angjelina Belaj, Raul de la Rosa, Ignacio J. Lorite, Roberto Mariotti, Nicolò G. M. Cultrera, Carmen R. Beuzón, J. J. González-Plaza, A. Muñoz-Mérida, O. Trelles, Luciana Baldoni
Published in: Frontiers in Plant Science, 9, 2018, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2018.01320
Author(s): Cippitani Roberto
Published in: Diritto e Processo - Derecho y Proceso - right & remedies, 2016, 2016, Page(s) 261-288, ISSN 1722-1110
Publisher: Università degli Studi di Perugia
DOI: 10.5281/zenodo.2567574
Author(s): Chiappini Daniele
Published in: Diritto e Processo - Derecho y Proceso - right & remedies, 2016, 2016, Page(s) 345-366, ISSN 1722-1110
Publisher: Università degli Studi di Perugia
DOI: 10.5281/zenodo.2585874
Author(s): Colcelli Valentina
Published in: Diritto e Processo - Derecho y Proceso - right & remedies, 2016, 2016, Page(s) 393-404, ISSN 1722-1110
Publisher: Università degli Studi di Perugia
DOI: 10.5281/zenodo.2571551
Author(s): Cecilia Mayorga Muniz, Hellen PaCHeCo-Cornejo, Ferdinando Treggiari.
Published in: Revista Jurídicas, 2017, 2017, Page(s) 29-45, ISSN 1794-2918
Publisher: Universidad de Caldas
DOI: 10.5281/zenodo.2591003
Author(s): Soraya Mousavi, Luca Regni, Marika Bocchini, Roberto Mariotti, Nicolò G. M. Cultrera, Stefano Mancuso, Jalaladdin Googlani, Mohammad Reza Chakerolhosseini, Consolación Guerrero, Emidio Albertini, Luciana Baldoni, Primo Proietti
Published in: Scientific Reports, 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-018-37496-5
Author(s): Nicolò G. M. Cultrera, Vania Sarri, Livia Lucentini, Marilena Ceccarelli, Fiammetta Alagna, Roberto Mariotti, Soraya Mousavi, Consolacion Guerrero Ruiz, Luciana Baldoni
Published in: Frontiers in Plant Science, 9, 2019, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2018.01932
Author(s): Pierre Saumitou-Laprade,
Philippe Vernet,
Xavier Vekemans,
Sylvain Billiard,
Sophie Gallina,
Laila Essalouh,
Ali Mhaïs,
Abdelmajid Moukhli,
Ahmed El Bakkali,
Gianni Barcaccia,
Fiammetta Alagna,
Roberto Mariotti,
Nicolò G.M. Cultrera,
Saverio Pandolfi,
Martina Rossi,
Bouchaïb Khadari,
Luciana Baldoni
Published in: Evolutionary Applications, 2017, 2017, ISSN 1752-4571
Publisher: Evolutionary Applications - Wiley Online Library
DOI: 10.1111/eva.12457
Author(s): Soraya Mousavi, Vitale Stanzione, Massimo Mencuccini, Luciana Baldoni, Marina Bufacchi, Roberto Mariotti
Published in: European Food Research and Technology, 245/1, 2019, Page(s) 83-94, ISSN 1438-2377
Publisher: Springer Verlag
DOI: 10.1007/s00217-018-3142-0
Author(s): Soraya Mousavi, Raul de la Rosa, Abdelmajid Moukhli, Milad El Riachy, Roberto Mariotti, Mariela Torres, Pierluigi Pierantozzi, Vitale Stanzione, Valerio Mastio, Hayat Zaher, Abderraouf El Antari, Salam Ayoub, Faten Dandachi, Hiyam Youssef, Nikolas Aggelou, Cibeles Contreras, Damián Maestri, Angjelina Belaj, Marina Bufacchi, Luciana Baldoni, Lorenzo Leon
Published in: Scientific Reports, 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-53169-3
Author(s): Ahmed El Bakkali, Laila Essalouh, Christine Tollon, Ronan Rivallan, Pierre Mournet, Abdelmajid Moukhli, Hayat Zaher, Abderrahmane Mekkaoui, Amal Hadidou, Lhassane Sikaoui, Bouchaib Khadari
Published in: PLOS ONE, 14/10, 2019, Page(s) e0223716, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0223716
Author(s): Soraya Mousavi, Roberto Mariotti, Luca Regni, Luigi Nasini, Marina Bufacchi, Saverio Pandolfi, Luciana Baldoni, Primo Proietti
Published in: Frontiers in Plant Science, 8, 2017, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2017.01283
Author(s): Cippitani Roberto
Published in: Anais do III Seminário internacional “Direitos fundamentais, jurisdição e processo coletivo”, 6, 2016
Publisher: VirtualBooks Editora
DOI: 10.5281/zenodo.2567580
Author(s): Cippitani Roberto
Published in: Biodiversidad y conocimientos tradicionales, 2018, 2018, Page(s) 125 - 148, ISBN 978-956-236-350-1
Publisher: Ediciones Universidad de la Frontera
DOI: 10.5281/zenodo.2567573
Author(s): Valentina Colcelli
Published in: Biodiversidad y conocimientos tradicionales, 2018, 2018, Page(s) 167-186, ISBN 978-956-236-350-1
Publisher: Ediciones Universidad de la Frontera
DOI: 10.5281/zenodo.2571521
Author(s): Valentina Colcelli
Published in: "Constitutional Courts and Ordinary Courts: Cooperation or Conflict?""- XVIIth International Congress on European and Comparative Constitutional Law", 2017, 2017, Page(s) 321-332, ISBN 978-3-86845-147-4
Publisher: Universitätsverlag Regensburg
DOI: 10.5281/zenodo.2571541
Author(s): Mayorga Muñoz Cecilia; Treggiari Ferdinando
Published in: Biodiversidad y Conocimimientos Tradicionales, 2018, 2018, ISBN 978-956-236-350-1
Publisher: Ediciones Universidad de la Frontera
DOI: 10.5281/zenodo.2567582