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Bioresources For Oliviculture

Deliverables

Core set of genotypes present in the main olive cultivar collections and grown under different agro-environmental conditions to evaluate their agronomical performance

A subset of cultivars, common to all collections will be considered for the following aspects: tree shape and behavior, variation on oil acidity composition and polyphenols content, abiotic stress tolerance and fruit production.

Capacity of main olive varieties and wild plants to face drought stress

All information available on the potential to grow under drought stress conditions will be organized into the common database.

Capacity of main olive varieties and wild plants to face salt stress

All information available on the potential to grow under salt stress conditions will be organized into the common database.

Common protocol to collect morphological parameters

A common protocol to collect morphological parameters will be established. Aspects to consider include the number and type of traits, phenology, representative samples, imaging technology, softwares for data elaboration and data output.

Identification of the most relevant aspects to explore by association mapping strategies

Main traits that will be explored include: oil quantity, polyphenols content, acidity composition, tree habit, fruit size, pulp/stone ratio, harvesting season, drought and cold tolerance, diseases (Spilocaea oleagina, Pseudomonas savastanoi, Verticillium dahliae, etc). Markers linked to these traits will be searched by the approach of association mapping.

Phenotypic profile of varieties related to tree vigour and canopy shape

All information available on tree vigor and canopy shape will be organized into the common database.

Inter-incompatibility for the most important 200 varieties

Data made available on inter-incompatibility of cultivars will be organized into the common database.

Development of new cytoplasmic (plastidial and mitochondrial) markers able to discriminate cultivars, wild plants and related subspecies

A new set of chloroplast markers will be developed starting from the set already published, including those able to discriminate among wild plants and related subspecies. A new set of cytoplasmic markers will be also made available from the whole mitochondrial genome sequence.

Phenotypic profile of varieties related to fatty acid olive oil composition

Data available on oil fatty acid composition will be collected for each cultivar and will be compared among the main collections considering other aspects such as: area of diffusion, altitude, latitude and climatic conditions, etc.

Capacity of main olive varieties and wild plants to face heat stress

All information available on the potential to grow under heat stress conditions will be organized into the common database.

Rules concerning the access rights

LRU will Identify the legal and ethical problems linked of the construction of the databank. In particular the problems as follows will be taken into consideration: - Problems concerning the property and the other rights relating the databank and the genetic material; - Problems related to the access rights.

Development of new polynucleotide-SSR markers able to discriminate among cultivars

A selection based on their discrimination power of poly-nucleotide SSR as tri-, tetra- and penta-nucleotide repeats will be done, from the whole genome sequence and from the numerous RNA libraries made available by partners

Best varieties associations for the most relevant olive producing areas

All information available on the best association of cultivars related to climatic, soil and biotic/abiotic stress resistance for the most relevant olive producing areas will be organized into the common database.

Phenotypic profile of varieties related to the early bearing

All information available on early bearing will be organized into the common database.

Core list of varieties (about 200) representing the main olive oil producing countries

A list of the most relevant varieties contributing to the olive oil production for the Mediterranean countries and new areas of olive cultivation of South (Argentina and Chile) and North America (Mexico) will be provided. Reference information will derive from International, National and Regional Olive Varietal collections previously characterized by molecular and morphological markers.

Molecular profiles of olive varieties previously characterized for agronomical/phenotypical character

Markers linked to traits of agronomical, morphological or biological interest will also be searched in order to assign a specific trait to a relative marker

Self-incompatibility for the most important 200 varieties

Data made available on self-incompatibility of cultivars will be organized into the common database.

Phenotypic profile of varieties (resistant/susceptible) related to the olive leaf spot (Spilocaea oleagina) disease

Data available for each cultivar on susceptibility to olive leaf spot will be compared among the main collections considering other aspects such as: area of diffusion, climatic conditions, pathogen strain, etc.

Development of new SNP markers able to discriminate among cultivars

The best SNP (Single Nucleotide Polymorphisms) markers, developed by the partners of the project by using Next Generation Sequencing (NGS) approach, will be selected to establish a common set of markers for cultivar discrimination and to build a SNP array.

Awareness and Dissemination plan

An awareness and dissemination plan will be elaborated and will be updated and deployed along the project life and beyond. The different versions of the Dissemination Plan will include the activities that have actually been developed in the project in the previous plan and those foreseen for the next period. The Dissemination Plan will contain the following elements: 1) Identification and classification of target stakeholders to be addressed. 2) The dissemination methods and their specific associated activities. 3) Schedule and complementarily of the dissemination activities among partners. 4) The conditions to ensure proper dissemination of the generated knowledge. As was previously commented, the identification and communication with stakeholders is of main importance. The first Awareness and Dissemination Plan will include a list of stakeholders and the plan to address those, a close contact must be established, offering information in different formats in order to ensure it adequacy to the needs of each group of interest. This kind of contact will start early in the life of the project

Phenotypic profile of varieties (resistant/susceptible) related to the olive fruit fly (Bactrocera oleae) insect

Data available for each cultivar on susceptibility to olive fruit fly will be compared among the main collections considering other aspects such as: insect diffusion, number of generations, meteorological parameters, level of damage, etc.

Final release of a common set of molecular profiles of the olive cultivars, wild populations and related subspecies

The same action, previously described, will include also wild plants and related subspecies discriminated by a specific marker set.

Phenotypic profile of varieties (resistant/susceptible) related to the Verticillium (Verticillium dahliae) disease

Data available for each cultivar on susceptibility to Verticillium wilt will be compared among the main collections considering other aspects such as: area of diffusion, irrigation, tree damages, etc.

Phenotypic profile of varieties related to biophenols, secoiridoids, steroids and tocoferols olive oil composition

Data on oil composition in biophenols, secoiridoids, steroids and tocoferols will be reported for each cultivar on a common database.

Economical and Institutional aspects second release

A second and final release of the economical and institutional aspects at the end of the analysis.

First release of a common set of molecular profiles of the olive cultivars

From the analysis of 200 main cultivars with the set of markers selected within this project it will be possible to obtain a unique and stable profile for each variety, accepted as reference profile at world level.

Phenotypic profile of varieties related to the varietal use

All other information available on the varietal use, such as table olive varieties, oil characteristics, varieties showing strong adaptation to extreme conditions, will be organized into the common database..

Seminars and Workshops related to each secondment of WP2

Seminars and Workshops will be organized in correspondence of each deliverable.

First release of a molecular profiles database for olive cultivars

A common database including all cultivar profiles will be built, able to compare olive varieties for their identification and discrimination.

Workshops and seminars of WP4

Seminars and Workshops will be organized at the end of each secondment

Final release of a common molecular database for olive cultivars

The final Database version including the molecular profiles of cultivars, wild plants and related subspecies will be definitively published.

Public Website

Project website “BeFOre” project will have its own web page, developed and maintained by ISAFOM. It will be used for the internal communication, and it will also form the basis for the administrative and the external communication. A book reporting the main results will be published on the web site of the project; Internal information will be restricted to partners, which have different rights for viewing parts of the information structure. The web site will also be used as a basic tool for external communications. In the public area we will develop a communication strategy towards the general public and selected target group. Here we will collect all material, which should be publicly accessible (presentations, papers, information material, posters, etc).

Establishment of a highthroughput platform for olive cultivars molecular characterization

Based on the markers developed, a common platform will be established to discriminate among olive cultivars, wild plants and related subspecies in each lab involved in the project.

Final release and online publication of a common database on morphological parameters

The final release of a common database will be done as an online free access version and as publications.

Searching for OpenAIRE data...

Publications

Description of olive morphological parameters by using open access software

Author(s): Konstantinos N. Blazakis, Maria Kosma, George Kostelenos, Luciana Baldoni, Marina Bufacchi, Panagiotis Kalaitzis
Published in: Plant Methods, Issue 13/1, 2017, ISSN 1746-4811
DOI: 10.1186/s13007-017-0261-8

Venetian olive (Olea europaea) germplasm: disclosing the genetic identity of locally grown cultivars suited for typical extra virgin oil productions

Author(s): Ibrahim Hmmam, Roberto Mariotti, Benedetto Ruperti, Nicolò Cultrera, Luciana Baldoni, Gianni Barcaccia
Published in: Genetic Resources and Crop Evolution, Issue 65/6, 2018, Page(s) 1733-1750, ISSN 0925-9864
DOI: 10.1007/s10722-018-0650-5

Development, evaluation, and validation of new EST-SSR markers in olive (Olea europaea L.)

Author(s): R. Mariotti, N. G. M. Cultrera, S. Mousavi, F. Baglivo, M. Rossi, E. Albertini, F. Alagna, F. Carbone, G. Perrotta, Luciana Baldoni
Published in: Tree Genetics & Genomes, Issue 12/6, 2016, ISSN 1614-2942
DOI: 10.1007/s11295-016-1077-9

Using Wild Olives in Breeding Programs: Implications on Oil Quality Composition

Author(s): Lorenzo León, Raúl de la Rosa, Leonardo Velasco, Angjelina Belaj
Published in: Frontiers in Plant Science, Issue 9, 2018, ISSN 1664-462X
DOI: 10.3389/fpls.2018.00232

The eastern part of the Fertile Crescent concealed an unexpected route of olive (Olea europaea L.) differentiation

Author(s): Soraya Mousavi, Roberto Mariotti, Francesca Bagnoli, Lorenzo Costantini, Nicolò G. M. Cultrera, Kazem Arzani, Saverio Pandolfi, Giovanni Giuseppe Vendramin, Bahareh Torkzaban, Mehdi Hosseini-Mazinani, Luciana Baldoni
Published in: Annals of Botany, Issue 119/8, 2017, Page(s) 1305-1318, ISSN 0305-7364
DOI: 10.1093/aob/mcx027

Usefulness of a New Large Set of High Throughput EST-SNP Markers as a Tool for Olive Germplasm Collection Management

Author(s): Angjelina Belaj, Raul de la Rosa, Ignacio J. Lorite, Roberto Mariotti, Nicolò G. M. Cultrera, Carmen R. Beuzón, J. J. González-Plaza, A. Muñoz-Mérida, O. Trelles, Luciana Baldoni
Published in: Frontiers in Plant Science, Issue 9, 2018, ISSN 1664-462X
DOI: 10.3389/fpls.2018.01320

«Property paradigm» and protection of rights concerning genetic information

Author(s): Cippitani Roberto
Published in: Diritto e Processo - Derecho y Proceso - right & remedies, Issue 2016, 2016, Page(s) 261-288, ISSN 1722-1110
DOI: 10.5281/zenodo.2567574

THE XYLELLA FASTIDIOSA CASE: A WAY FOR THINKING ON RESEARCH DAMAGE IN THE ITALIAN LEGAL SYSTEM

Author(s): Chiappini Daniele
Published in: Diritto e Processo - Derecho y Proceso - right & remedies, Issue 2016, 2016, Page(s) 345-366, ISSN 1722-1110
DOI: 10.5281/zenodo.2585874

Protección de la biodiversidad y conocimiento tradicionale asociados a losrecursos genético en la «Societad del Conocimiento»

Author(s): Colcelli Valentina
Published in: Diritto e Processo - Derecho y Proceso - right & remedies, Issue 2016, 2016, Page(s) 393-404, ISSN 1722-1110
DOI: 10.5281/zenodo.2571551

EL ROL DE LA MUJER INDÍGENA MAPUCHE EN LA PRESERVACIÓN DE RECURSOS GENÉTICOS Y CONOCIMIENTOS TRADICIONALES ASOCIADOS. UN ANÁLISIS JURÍDICO DESDE LA PERSPECTIVA DE GÉNERO

Author(s): Cecilia Mayorga Muniz, Hellen PaCHeCo-Cornejo, Ferdinando Treggiari.
Published in: Revista Jurídicas, Issue 2017, 2017, Page(s) 29-45, ISSN 1794-2918
DOI: 10.5281/zenodo.2591003

Physiological, epigenetic and genetic regulation in some olive cultivars under salt stress

Author(s): Soraya Mousavi, Luca Regni, Marika Bocchini, Roberto Mariotti, Nicolò G. M. Cultrera, Stefano Mancuso, Jalaladdin Googlani, Mohammad Reza Chakerolhosseini, Consolación Guerrero, Emidio Albertini, Luciana Baldoni, Primo Proietti
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
DOI: 10.1038/s41598-018-37496-5

High Levels of Variation Within Gene Sequences of Olea europaea L.

Author(s): Nicolò G. M. Cultrera, Vania Sarri, Livia Lucentini, Marilena Ceccarelli, Fiammetta Alagna, Roberto Mariotti, Soraya Mousavi, Consolacion Guerrero Ruiz, Luciana Baldoni
Published in: Frontiers in Plant Science, Issue 9, 2019, ISSN 1664-462X
DOI: 10.3389/fpls.2018.01932

Elucidation of the genetic architecture of self-incompatibility in olive: evolutionary consequences and perspectives for orchard management

Author(s): Pierre Saumitou-Laprade, Philippe Vernet, Xavier Vekemans, Sylvain Billiard, Sophie Gallina, Laila Essalouh, Ali Mhaïs, Abdelmajid Moukhli, Ahmed El Bakkali, Gianni Barcaccia, Fiammetta Alagna, Roberto Mariotti, Nicolò G.M. Cultrera, Saverio Pandolfi, Martina Rossi, Bouchaïb Khadari, Luciana Baldoni
Published in: Evolutionary Applications, Issue 2017, 2017, ISSN 1752-4571
DOI: 10.1111/eva.12457

Biochemical and molecular profiling of unknown olive genotypes from central Italy: determination of major and minor components

Author(s): Soraya Mousavi, Vitale Stanzione, Massimo Mencuccini, Luciana Baldoni, Marina Bufacchi, Roberto Mariotti
Published in: European Food Research and Technology, Issue 245/1, 2019, Page(s) 83-94, ISSN 1438-2377
DOI: 10.1007/s00217-018-3142-0

Plasticity of fruit and oil traits in olive among different environments

Author(s): Soraya Mousavi, Raul de la Rosa, Abdelmajid Moukhli, Milad El Riachy, Roberto Mariotti, Mariela Torres, Pierluigi Pierantozzi, Vitale Stanzione, Valerio Mastio, Hayat Zaher, Abderraouf El Antari, Salam Ayoub, Faten Dandachi, Hiyam Youssef, Nikolas Aggelou, Cibeles Contreras, Damián Maestri, Angjelina Belaj, Marina Bufacchi, Luciana Baldoni, Lorenzo Leon
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
DOI: 10.1038/s41598-019-53169-3

Characterization of Worldwide Olive Germplasm Banks of Marrakech (Morocco) and Córdoba (Spain): Towards management and use of olive germplasm in breeding programs

Author(s): Ahmed El Bakkali, Laila Essalouh, Christine Tollon, Ronan Rivallan, Pierre Mournet, Abdelmajid Moukhli, Hayat Zaher, Abderrahmane Mekkaoui, Amal Hadidou, Lhassane Sikaoui, Bouchaib Khadari
Published in: PLOS ONE, Issue 14/10, 2019, Page(s) e0223716, ISSN 1932-6203
DOI: 10.1371/journal.pone.0223716

The First Molecular Identification of an Olive Collection Applying Standard Simple Sequence Repeats and Novel Expressed Sequence Tag Markers

Author(s): Soraya Mousavi, Roberto Mariotti, Luca Regni, Luigi Nasini, Marina Bufacchi, Saverio Pandolfi, Luciana Baldoni, Primo Proietti
Published in: Frontiers in Plant Science, Issue 8, 2017, ISSN 1664-462X
DOI: 10.3389/fpls.2017.01283

«PATRIMONIO GENÉTICO» Y DERECHOS FUNDAMENTALES

Author(s): Cippitani Roberto
Published in: Anais do III Seminário internacional “Direitos fundamentais, jurisdição e processo coletivo”, Issue 6, 2016
DOI: 10.5281/zenodo.2567580

La investigación científica sobre los recursos genéticos: reflexiones jurídicas

Author(s): Cippitani Roberto
Published in: Biodiversidad y conocimientos tradicionales, Issue 2018, 2018, Page(s) 125 - 148
DOI: 10.5281/zenodo.2567573

Implementando el Protocolo de Nagoya entre la Unión Europea.

Author(s): Valentina Colcelli
Published in: Biodiversidad y conocimientos tradicionales, Issue 2018, 2018, Page(s) 167-186
DOI: 10.5281/zenodo.2571521

European Court of Human Rights and national Courts: the case of Nagoya Protocol, vegetable patents granted under the Regulation (EU) n. 511/2014

Author(s): Valentina Colcelli
Published in: "Constitutional Courts and Ordinary Courts: Cooperation or Conflict?""- XVIIth International Congress on European and Comparative Constitutional Law", Issue 2017, 2017, Page(s) 321-332
DOI: 10.5281/zenodo.2571541

Biodiversidad y Conocimimientos Tradicionales

Author(s): Mayorga Muñoz Cecilia; Treggiari Ferdinando
Published in: Biodiversidad y Conocimimientos Tradicionales, Issue 2018, 2018
DOI: 10.5281/zenodo.2567582