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Next generation single molecule protein fluorescence

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FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

Author(s): Eitan Lerner, Anders Barth, Jelle Hendrix, Benjamin Ambrose, Victoria Birkedal, Scott C Blanchard, Richard Börner, Hoi Sung Chung, Thorben Cordes, Timothy D Craggs, Ashok A Deniz, Jiajie Diao, Jingyi Fei, Ruben L Gonzalez, Irina V Gopich, Taekjip Ha, Christian A Hanke, Gilad Haran, Nikos S Hatzakis, Sungchul Hohng, Seok-Cheol Hong, Thorsten Hugel, Antonino Ingargiola, Chirlmin Joo, Achillefs N Ka
Published in: eLife, 10, 2021, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.60416

Synthesis and Evaluation of Novel Ring‐Strained Noncanonical Amino Acids for Residue‐Specific Bioorthogonal Reactions in Living Cells

Author(s): Christopher D. Reinkemeier, Christine Koehler, Paul F. Sauter, Nataliia V. Shymanska, Cecile Echalier, Anna Rutkowska, David W. Will, Carsten Schultz, Edward A. Lemke
Published in: Chemistry – A European Journal, 27/19, 2021, Page(s) 6094-6099, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.202100322

Multifunctionality of F-rich nucleoporins

Author(s): Nike Heinß, Mikhail Sushkin, Miao Yu, Edward A. Lemke
Published in: Biochemical Society Transactions, 48/6, 2020, Page(s) 2603-2614, ISSN 0300-5127
Publisher: Portland Press, Ltd.
DOI: 10.1042/bst20200357

Floppy but not sloppy: Interaction mechanism of FG-nucleoporins and nuclear transport receptors

Author(s): Iker Valle Aramburu, Edward A. Lemke
Published in: Seminars in Cell & Developmental Biology, 68, 2017, Page(s) 34-41, ISSN 1084-9521
Publisher: Academic Press
DOI: 10.1016/j.semcdb.2017.06.026

Beyond the Transport Function of Import Receptors: What’s All the FUS about?

Author(s): Sofya Mikhaleva, Edward A. Lemke
Published in: Cell, 173/3, 2018, Page(s) 549-553, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1016/j.cell.2018.04.002

Two Differential Binding Mechanisms of FG-Nucleoporins and Nuclear Transport Receptors

Author(s): Piau Siong Tan, Iker Valle Aramburu, Davide Mercadante, Swati Tyagi, Aritra Chowdhury, Daniel Spitz, Sarah L. Shammas, Frauke Gräter, Edward A. Lemke
Published in: Cell Reports, 22/13, 2018, Page(s) 3660-3671, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2018.03.022

Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study

Author(s): Björn Hellenkamp, Sonja Schmid, Olga Doroshenko, Oleg Opanasyuk, Ralf Kühnemuth, Soheila Rezaei Adariani, Benjamin Ambrose, Mikayel Aznauryan, Anders Barth, Victoria Birkedal, Mark E. Bowen, Hongtao Chen, Thorben Cordes, Tobias Eilert, Carel Fijen, Christian Gebhardt, Markus Götz, Giorgos Gouridis, Enrico Gratton, Taekjip Ha, Pengyu Hao, Christian A. Hanke, Andreas Hartmann, Jelle Hendrix, Lass
Published in: Nature Methods, 15/9, 2018, Page(s) 669-676, ISSN 1548-7091
Publisher: Nature Publishing Group
DOI: 10.1038/s41592-018-0085-0

Mechanism-Dependent Modulation of Ultrafast Interfacial Water Dynamics in Intrinsically Disordered Protein Complexes

Author(s): Aritra Chowdhury, Sergey A. Kovalenko, Iker Valle Aramburu, Piau Siong Tan, Nikolaus P. Ernsting, Edward A. Lemke
Published in: Angewandte Chemie International Edition, 58/14, 2019, Page(s) 4720-4724, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201813354

Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes

Author(s): Christopher D. Reinkemeier, Gemma Estrada Girona, Edward A. Lemke
Published in: Science, 363/6434, 2019, Page(s) eaaw2644, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.aaw2644

Comment on “Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water”

Author(s): Gustavo Fuertes, Niccolo Banterle, Kiersten M. Ruff, Aritra Chowdhury, Rohit V. Pappu, Dmitri I. Svergun, Edward A. Lemke
Published in: Science, 361/6405, 2018, Page(s) eaau8230, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.aau8230

Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy

Author(s): Ivana Nikić, Gemma Estrada Girona, Jun Hee Kang, Giulia Paci, Sofya Mikhaleva, Christine Koehler, Nataliia V. Shymanska, Camilla Ventura Santos, Daniel Spitz, Edward A. Lemke
Published in: Angewandte Chemie International Edition, 55/52, 2016, Page(s) 16172-16176, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201608284

Nanoscale devices for linkerless long-term single-molecule observation

Author(s): Niccolò Banterle, Edward A Lemke
Published in: Current Opinion in Biotechnology, 39, 2016, Page(s) 105-112, ISSN 0958-1669
Publisher: Elsevier BV
DOI: 10.1016/j.copbio.2016.02.013

The Multiple Faces of Disordered Nucleoporins

Author(s): Edward A. Lemke
Published in: Journal of Molecular Biology, 428/10, 2016, Page(s) 2011-2024, ISSN 0022-2836
Publisher: Academic Press
DOI: 10.1016/j.jmb.2016.01.002

The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes

Author(s): Giorgia Celetti, Giulia Paci, Joana Caria, Virginia VanDelinder, George Bachand, Edward A. Lemke
Published in: The Journal of Cell Biology, 219/1, 2020, ISSN 0021-9525
Publisher: Rockefeller University Press
DOI: 10.1083/jcb.201907157

Inducible Genetic Code Expansion in Eukaryotes

Author(s): Christine Koehler, Gemma Estrada Girona, Christopher D. Reinkemeier, Edward A. Lemke
Published in: ChemBioChem, 21/22, 2020, Page(s) 3216-3219, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000338

Probing Differential Binding Mechanisms of Phenylalanine-Glycine-Rich Nucleoporins by Single-Molecule FRET

Author(s): Piau Siong Tan, Edward A. Lemke
Published in: Intrinsically Disordered Proteins, 611, 2018, Page(s) 327-346, ISBN 9780-128156490
Publisher: Elsevier
DOI: 10.1016/bs.mie.2018.08.034