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Next generation single molecule protein fluorescence


Floppy but not sloppy: Interaction mechanism of FG-nucleoporins and nuclear transport receptors

Author(s): Iker Valle Aramburu, Edward A. Lemke
Published in: Seminars in Cell & Developmental Biology, Issue 68, 2017, Page(s) 34-41, ISSN 1084-9521
DOI: 10.1016/j.semcdb.2017.06.026

Beyond the Transport Function of Import Receptors: What’s All the FUS about?

Author(s): Sofya Mikhaleva, Edward A. Lemke
Published in: Cell, Issue 173/3, 2018, Page(s) 549-553, ISSN 0092-8674
DOI: 10.1016/j.cell.2018.04.002

Two Differential Binding Mechanisms of FG-Nucleoporins and Nuclear Transport Receptors

Author(s): Piau Siong Tan, Iker Valle Aramburu, Davide Mercadante, Swati Tyagi, Aritra Chowdhury, Daniel Spitz, Sarah L. Shammas, Frauke Gräter, Edward A. Lemke
Published in: Cell Reports, Issue 22/13, 2018, Page(s) 3660-3671, ISSN 2211-1247
DOI: 10.1016/j.celrep.2018.03.022

Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study

Author(s): Björn Hellenkamp, Sonja Schmid, Olga Doroshenko, Oleg Opanasyuk, Ralf Kühnemuth, Soheila Rezaei Adariani, Benjamin Ambrose, Mikayel Aznauryan, Anders Barth, Victoria Birkedal, Mark E. Bowen, Hongtao Chen, Thorben Cordes, Tobias Eilert, Carel Fijen, Christian Gebhardt, Markus Götz, Giorgos Gouridis, Enrico Gratton, Taekjip Ha, Pengyu Hao, Christian A. Hanke, Andreas Hartmann, Jelle Hendrix, Lass
Published in: Nature Methods, Issue 15/9, 2018, Page(s) 669-676, ISSN 1548-7091
DOI: 10.1038/s41592-018-0085-0

Mechanism-Dependent Modulation of Ultrafast Interfacial Water Dynamics in Intrinsically Disordered Protein Complexes

Author(s): Aritra Chowdhury, Sergey A. Kovalenko, Iker Valle Aramburu, Piau Siong Tan, Nikolaus P. Ernsting, Edward A. Lemke
Published in: Angewandte Chemie International Edition, Issue 58/14, 2019, Page(s) 4720-4724, ISSN 1433-7851
DOI: 10.1002/anie.201813354

Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes

Author(s): Christopher D. Reinkemeier, Gemma Estrada Girona, Edward A. Lemke
Published in: Science, Issue 363/6434, 2019, Page(s) eaaw2644, ISSN 0036-8075
DOI: 10.1126/science.aaw2644

Comment on “Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water”

Author(s): Gustavo Fuertes, Niccolo Banterle, Kiersten M. Ruff, Aritra Chowdhury, Rohit V. Pappu, Dmitri I. Svergun, Edward A. Lemke
Published in: Science, Issue 361/6405, 2018, Page(s) eaau8230, ISSN 0036-8075
DOI: 10.1126/science.aau8230

Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy

Author(s): Ivana Nikić, Gemma Estrada Girona, Jun Hee Kang, Giulia Paci, Sofya Mikhaleva, Christine Koehler, Nataliia V. Shymanska, Camilla Ventura Santos, Daniel Spitz, Edward A. Lemke
Published in: Angewandte Chemie International Edition, Issue 55/52, 2016, Page(s) 16172-16176, ISSN 1433-7851
DOI: 10.1002/anie.201608284

Nanoscale devices for linkerless long-term single-molecule observation

Author(s): Niccolò Banterle, Edward A Lemke
Published in: Current Opinion in Biotechnology, Issue 39, 2016, Page(s) 105-112, ISSN 0958-1669
DOI: 10.1016/j.copbio.2016.02.013

The Multiple Faces of Disordered Nucleoporins

Author(s): Edward A. Lemke
Published in: Journal of Molecular Biology, Issue 428/10, 2016, Page(s) 2011-2024, ISSN 0022-2836
DOI: 10.1016/j.jmb.2016.01.002

The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes

Author(s): Giorgia Celetti, Giulia Paci, Joana Caria, Virginia VanDelinder, George Bachand, Edward A. Lemke
Published in: The Journal of Cell Biology, Issue 219/1, 2020, ISSN 0021-9525
DOI: 10.1083/jcb.201907157

Probing Differential Binding Mechanisms of Phenylalanine-Glycine-Rich Nucleoporins by Single-Molecule FRET

Author(s): Piau Siong Tan, Edward A. Lemke
Published in: Intrinsically Disordered Proteins, Issue 611, 2018, Page(s) 327-346
DOI: 10.1016/bs.mie.2018.08.034