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Next generation single molecule protein fluorescence

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Floppy but not sloppy: Interaction mechanism of FG-nucleoporins and nuclear transport receptors

Published in: ISSN 1084-9521
DOI: 10.1016/j.semcdb.2017.06.026

Beyond the Transport Function of Import Receptors: What’s All the FUS about?

Author(s): Sofya Mikhaleva, Edward A. Lemke
Published in: Cell, Issue 173/3, 2018, Page(s) 549-553, ISSN 0092-8674
DOI: 10.1016/j.cell.2018.04.002

Two Differential Binding Mechanisms of FG-Nucleoporins and Nuclear Transport Receptors

Author(s): Piau Siong Tan, Iker Valle Aramburu, Davide Mercadante, Swati Tyagi, Aritra Chowdhury, Daniel Spitz, Sarah L. Shammas, Frauke Gräter, Edward A. Lemke
Published in: Cell Reports, Issue 22/13, 2018, Page(s) 3660-3671, ISSN 2211-1247
DOI: 10.1016/j.celrep.2018.03.022

Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study

Author(s): Björn Hellenkamp, Sonja Schmid, Olga Doroshenko, Oleg Opanasyuk, Ralf Kühnemuth, Soheila Rezaei Adariani, Benjamin Ambrose, Mikayel Aznauryan, Anders Barth, Victoria Birkedal, Mark E. Bowen, Hongtao Chen, Thorben Cordes, Tobias Eilert, Carel Fijen, Christian Gebhardt, Markus Götz, Giorgos Gouridis, Enrico Gratton, Taekjip Ha, Pengyu Hao, Christian A. Hanke, Andreas Hartmann, Jelle Hendrix, Lass
Published in: Nature Methods, Issue 15/9, 2018, Page(s) 669-676, ISSN 1548-7091
DOI: 10.1038/s41592-018-0085-0

Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes

Author(s): Christopher D. Reinkemeier, Gemma Estrada Girona, Edward A. Lemke
Published in: Science, Issue 363/6434, 2019, Page(s) eaaw2644, ISSN 0036-8075
DOI: 10.1126/science.aaw2644

Comment on “Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water”

Author(s): Gustavo Fuertes, Niccolo Banterle, Kiersten M. Ruff, Aritra Chowdhury, Rohit V. Pappu, Dmitri I. Svergun, Edward A. Lemke
Published in: Science, Issue 361/6405, 2018, Page(s) eaau8230, ISSN 0036-8075
DOI: 10.1126/science.aau8230

Nanoscale devices for linkerless long-term single-molecule observation

Author(s): Niccolò Banterle, Edward A Lemke
Published in: Current Opinion in Biotechnology, Issue 39, 2016, Page(s) 105-112, ISSN 0958-1669
DOI: 10.1016/j.copbio.2016.02.013

The Multiple Faces of Disordered Nucleoporins

Author(s): Edward A. Lemke
Published in: Journal of Molecular Biology, Issue 428/10, 2016, Page(s) 2011-2024, ISSN 0022-2836
DOI: 10.1016/j.jmb.2016.01.002

The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes

Author(s): Giorgia Celetti, Giulia Paci, Joana Caria, Virginia VanDelinder, George Bachand, Edward A. Lemke
Published in: The Journal of Cell Biology, Issue 219/1, 2020, ISSN 0021-9525
DOI: 10.1083/jcb.201907157

Probing Differential Binding Mechanisms of Phenylalanine-Glycine-Rich Nucleoporins by Single-Molecule FRET

Author(s): Piau Siong Tan, Edward A. Lemke
Published in: Intrinsically Disordered Proteins, Issue 611, 2018, Page(s) 327-346
DOI: 10.1016/bs.mie.2018.08.034