Objectif In recent years it has become evident that the functional human proteome is vastly expanded by post-translational modifications (PTMs) that modify protein function and gene expression in a time- and context-dependent manner. Misregulation of these modifications leads to developmental disorders and often contributes to disease biogenesis. However, with the possible exception of protein phosphorylation, the full roles and regulation of these modifications remain poorly understood. Currently, in particular, arginine methylation is proposed to be largely stable and irreversible. Building on my exciting preliminary data that demonstrate that Jumonji C histone demethylases (JmjC KDMs) can catalyse removal of arginine methylation, this proposal aims to identify biologically relevant methylarginine substrates of the JmjC KDM. Cutting-edge mRNA display-based peptide screening technology will be adapted for use as a substrate identification assay in collaboration with Prof Hiroaki Suga at the University of Tokyo. Consensus sequences identified using this novel methodology will be used to identify biologically relevant peptide sequences by bioinformatic searches. On the return to the UK, results with isolated proteins will be correlated with in-cell studies and the functional consequences of these reactions assessed. Given the high level of interest in the post-translational modification field, the results of this study will be of interest to a very wide-range of scientists including other academic researchers in the field of epigenetics and beyond, and major biotechnology and pharmaceutical companies studying JmjC KDMs as drug targets. The fellowship will provide training in state-of-the-art techniques. The collaboration between the University of Oxford and the University of Tokyo will enhance the research capabilities of the European Union, promote cross-fertilisation of knowledge and strengthen research ties with Japan. Champ scientifique medical and health sciencesbasic medicinepharmacology and pharmacydrug discoverymedical and health sciencesclinical medicinerheumatologymedical and health sciencesclinical medicineoncologymedical and health sciencesbasic medicinepathologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Thème(s) MSCA-IF-2014-GF - Marie Skłodowska-Curie Individual Fellowships (IF-GF) Appel à propositions H2020-MSCA-IF-2014 Voir d’autres projets de cet appel Régime de financement MSCA-IF-GF - Global Fellowships Coordinateur THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Contribution nette de l'UE € 270 271,80 Adresse WELLINGTON SQUARE UNIVERSITY OFFICES OX1 2JD Oxford Royaume-Uni Voir sur la carte Région South East (England) Berkshire, Buckinghamshire and Oxfordshire Oxfordshire Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 270 271,80 Partenaires (1) Trier par ordre alphabétique Trier par contribution nette de l'UE Tout développer Tout réduire Partenaire Les organisations partenaires contribuent à la mise en œuvre de l’action, mais ne signent pas la convention de subvention. NATIONAL UNIVERSITY CORPORATION THEUNIVERSITY OF TOKYO Japon Contribution nette de l'UE € 0,00 Adresse HONGO BUNKYO KU 7 3 1 113 8656 Tokyo Voir sur la carte Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 178 544,40