Objective
Worldwide a large proportion of all new cases of HIV-1 infection are due to anal sexual intercourse, being the anal/rectal as well as distal colonic mucosa a site of viral uptake and entry. I recently demonstrated that intestinal lamina propria resident DCs migrate towards and extend cellular processes between the tight junctions of columnar epithelium through a CCR5-dependent mechanism, take up R5 but not X4 HIV-1, and then transfer infection to susceptible T cells. Although in mice CX3CR1+DCs are deputed to shuttle microbial antigens, there is no clear idea on which subsets of DCs are located in the human GI tract, are involved in HIV infection and contribute to virus spread, its presentation or possibly to its reservoir. Moreover, the ability of DCs to shuttle the virus across the mucosa may be largely modulated by components of semen, the natural vehicle of HIV during sexual transmission.
To fill these gaps I propose a comparative study in macaques and humans using a multidisciplinary approach, which will unravel the mechanism associated with rectal transmission of HIV/SIV by DCs and their modulation by semen.
Specific objectives will be:
1. Define the subsets of DCs and the molecular mechanism(s) through which DCs directly sample HIV-1/SIV through the intestinal epithelium and facilitate the spread of the infection.
2. Define the impact of semen on HIV/SIV-induced DCs migration across the epithelium.
3. Determine the ability of lamina propria DCs subsets to migrate across the intestinal epithelium and to redistribute to draining lymph-nodes following in vivo exposure of Cynomolgus macaques to infectious pathogenic SIV.
We expect to characterize the early events of DC-mediated intestinal transmission of HIV/SIV and thus contribute to the knowledge base needed to identify, evaluate and develop novel agents or strategies to prevent mucosal HIV transmission
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences zoology mammalogy primatology
- natural sciences biological sciences microbiology virology
- medical and health sciences health sciences infectious diseases RNA viruses HIV
- natural sciences physical sciences optics microscopy confocal microscopy
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
-
H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
See all projects funded under this programme -
H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
See all projects funded under this programme
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
See all projects funded under this funding scheme
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2014
See all projects funded under this callCoordinator
Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
75015 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.