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The unique dioxygenases of phytophagous spider mites: new enzyme players in plant-herbivore interactions?

Objective

Detoxification of poisonous xenobiotics in animals is typically performed by multi-gene enzyme families. Within arthropods, only insect genomes have been studied in detail where these families are well characterized. I recently uncovered a new enzyme family in the genome of a non-insect arthropod, the extremely polyphagous plant-feeding mite Tetranychus urticae and showed that this proliferated family was acquired via horizontal gene transfer from a fungal donor. The family codes for intradiol ring cleaving dioxygenases which cleave a particular set of aromatic structures, commonly found in pesticides and plant metabolites. Here, I propose to functionally characterize this exciting new gene family and to elucidate its role in xenobiotic detoxification, with a focus on plant secondary metabolites. First, to create a general picture, I will map the in situ expression of dioxygenases and time their responses to plant-derived secondary defense metabolites. Second, guided by preliminary results, I plan to study how these new herbivore enzymes counteract polyphenol oxidases, well-known plant defense enzymes that act against herbivores and target the same class of substrates. State-of-the-art plant transformation experiments will be performed in order to meticulously dissect their counterplay. Finally, by means of an unbiased multi-layered strategy, I will functionally characterize these new dioxygenases. Dioxygenases will be introduced into biological systems by functional expression in E. coli or insect cells, and by genetically transforming Drosophila. Cutting-edge differential metabolomics will identify the substrates and reaction products. By means of this project, I expect to unravel the selective advantage of this new family for phytophagous mites and open up avenues to exciting biotechnological applications which I expect to extend well beyond agriculture.

Field of science

  • /agricultural sciences/agriculture, forestry, and fisheries
  • /agricultural sciences/agriculture, forestry, and fisheries/agriculture
  • /medical and health sciences/medical biotechnology/genetic engineering/gene therapy
  • /natural sciences/biological sciences/genetics and heredity/genome
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes

Call for proposal

H2020-MSCA-IF-2014
See other projects for this call

Funding Scheme

MSCA-IF-EF-ST - Standard EF

Coordinator

UNIVERSITEIT VAN AMSTERDAM
Address
Spui 21
1012WX Amsterdam
Netherlands
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 165 598,80