Objective The impact of urbanisation on wildlife is of increasing global concern. It is well known that exposure to urban environmental stressors increases oxidative stress. More recently, exposure to pollutants has been shown to directly modify the epigenome, via DNA methylation, in humans. Oxidative stress may be a key mechanism mediating epigenetic modifications in response to pollutant exposure and thereby influencing development, disease resistance and ageing. This multidisciplinary project outlines the first study to date of the epigenetic basis of early-life effects in a wild bird exposed to urban environmental stress. Early-life stress exposure can have profound effects on phenotypic development and long-term fitness. Epigenetic mechanisms are influenced by environmental cues during development and therefore may mechanistically link developmental conditions with later-life performance. Using urban and rural populations of birds, we will perform experimental studies in the field and lab to examine whether increased oxidative stress during development modifies patterns of global and gene-specific DNA methylation and thereby alters genome stability (via DNA damage and telomere attrition) and expression of antioxidant genes. To achieve this, we propose a new framework to explain how the modification of DNA methylation and telomere length may be linked and decoupled by the direct and indirect effects of oxidative stress at different stages during development. Pilot data support this framework. This will enable us to disentangle the mechanistic relationships between exposure to urban stressors, oxidative stress, DNA methylation and telomere dynamics during development and the consequences for growth and survival in an altricial bird species. The project has implications for multiple EU policies and H2020 focus areas. By combining the researcher’s existing expertise with new skills and opportunities, the project will enhance career prospects and advance the research field. Fields of science natural sciencesbiological scienceszoologyornithologynatural sciencesbiological sciencesgeneticsDNAnatural sciencesearth and related environmental sciencessoil sciencesland-based treatmentnatural sciencesbiological sciencesgeneticsRNAnatural sciencesearth and related environmental sciencesatmospheric sciencesclimatologyclimatic changes Keywords urban ecology oxidative stress telomere dynamics environmental stress early-life stress avian DNA methylation Parus major Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF) Call for proposal H2020-MSCA-IF-2014 See other projects for this call Funding Scheme MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinator LUNDS UNIVERSITET Net EU contribution € 185 857,20 Address Paradisgatan 5c 22100 Lund Sweden See on map Region Södra Sverige Sydsverige Skåne län Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00
