Periodic Reporting for period 1 - NanoMATe (Nanoparticles for Molecular Imaging of Atherosclerosis: from Diagnosis to Treatment)
Reporting period: 2015-09-01 to 2017-08-31
A prospective rational for SERS based-diagnostics for atherosclerotic inflammation. A) The identification of inflammatory biomarkers in atherosclerotic plaques may be achieved via the intravenous injection of SERS-detectible bio-functionalised nanoparticles into a patient. Such nanoparticles would consist of; a noble metal core for surface enhancement of Raman spectra; a Raman reporter to provide a pre-determined Raman signature; a polymer coating (such as Polyethylene glycol, PEG) to reduce nanoparticle interaction with the immune system and prolong blood circulation time; a targeting molecule such as an antibody or aptamer to confer molecular specificity. These nanoparticles would circulate in the vasculature, binding to inflammatory biomarkers such as adhesion molecules, macrophage, T and B cell activation markers, and potentially atherosclerosis associated biomarkers. Following binding these nanoparticles would then be detectable at significant tissue depths, for example within the carotid artery, via surface-enhanced spatially offset Raman spectroscopy (SESORS). B) Following the injection of bio-functionalised nanoparticles and the investigation of atheroprone regions of the vasculature with SESORS, detection of biomarker specific bio-functionalised nanoparticles would be recorded. Consequently this would provide clinicians with a technology capable of detecting a panel of biomarkers suitable for subsequent correlation with disease severity and implementation as a potential predictor of disease outcome. A hypothetical example is provided whereby the detection of bio-functionalised nanoparticles indicated the presence of biomarkers A, D and E, and the absence of biomarkers B and C following SESORS investigation of a carotid artery. Reprinted from MacRitchie et al. Molecular imaging of atherosclerosis: spotlight on Raman spectroscopy and surface-enhanced Raman scattering. Heart. 2017. pii: heartjnl-2017-311447, distributed in accordance with the terms of the Creative Commons Attribution (CC BY 4.0) license.