Studying the bricks of microbial cities: characterization and structural properties of exopolysaccharides and their interaction with proteins and cations in anammox granular sludge

Informations projet

POLIS

N° de convention de subvention: 661429

DOI 10.3030/661429

Projet clôturé

Date de signature de la CE 22 Septembre 2015

Date de début 1 Decembre 2015

Date de fin 30 Novembre 2017

Financé au titre de

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coût total € 168 277,20

Contribution de l’UE € 168 277,20

Coordonné par

POLITECNICO DI MILANO
Italie

Objectif

The anaerobic ammonium oxidation (anammox) is an innovative technology for the removal of nitrogen from wastewater (ww) saving 60% of aeration and 100% of organic carbon. Its potential application to municipal ww allow a complete redesign of the present energy-consuming into an energy-yielding ww treatment plant. Due to the slow growth rate of anammox bacteria, their retention in the system, and therefore granule stability, is the main concern of this process. Granular stability is closely related to the properties of their exopolysaccharides (PS) and their interactions with proteins and mono-divalent cations. In the proposed research integrated analytical methods (UV-visible, FT-IR, MALDI-TOF MS, NMR, (pyrolysis)GC-MS, HPLC) will be used to investigate functional PS and their fine structures, unrevealing the mechanisms involved in anammox granular sludge formation and stability. Modern rheological (AOS, LLCT and Microrheology combined with CLSM), light scattering (DLS, SLS) and microscopy techniques (AFM, FT-IR, CLSM) will be used to investigate their interactions with proteins and cations. PS will be isolated from lab- and full-scale reactors. The definition of standardized extraction (loosely/tightly bound-EPS, LB/TB-EPS) and analytical methods will bridge the existing gap for efficient and meaningful comparison of the results from this and future research. The methods set up here, will largely contribute to increase the scientific significance of PS studies in anammox granular sludge area as well as facilitate the entire biofilm research field. Studies on biofouling and biocorrosion will also benefit from the high-quality of the information and basic knowledge acquired. This research will strengthen the EU leadership in anammox granular sludge technology also improving EU scientific excellence in PS research area and provides the researcher an advanced level of multidisciplinary training, opening concrete perspectives for his career development.

Champ scientifique (EuroSciVoc)

CORDIS classe les projets avec EuroSciVoc, une taxonomie multilingue des domaines scientifiques, grâce à un processus semi-automatique basé sur des techniques TLN. Voir: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

• sciences naturelles › sciences biologiques › biochimie › biomolécule › protéines › protéomique
• sciences naturelles › sciences biologiques › microbiologie › bactériologie
• sciences naturelles › sciences physiques › optique › spectroscopie › spectroscopie d’émission
• sciences naturelles › sciences chimiques › chimie analytique › calorimétrie
• sciences naturelles › sciences biologiques › biochimie › biomolécule › glucides

Vous devez vous identifier ou vous inscrire pour utiliser cette fonction

Programme(s)

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility

Thème(s)

• MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF)

Appel à propositions

H2020-MSCA-IF-2014

Régime de financement

MSCA-IF-EF-ST -

Coordinateur

POLITECNICO DI MILANO

• Contribution nette de l'UE: € 168 277,20
• Adresse: PIAZZA LEONARDO DA VINCI 32 ; 20133 Milano ; Italie
• Région: Nord-Ovest › Lombardia › Milano
• Type d’activité: Établissements d’enseignement supérieur ou secondaire
• Coût total: € 168 277,20