Reconstructing human oral microbiome composition changes over the last 8000 years with proteomics and metagenomics approaches

Información del proyecto

PROMETEO

Identificador del acuerdo de subvención: 661641

DOI

10.3030/661641

Proyecto finalizado el 11 Octubre 2016

Fecha de la firma de la CE 11 Marzo 2015

Fecha de inicio 1 Septiembre 2016

Fecha de finalización 31 Agosto 2018

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 200 194,80

Aportación de la UE

€ 200 194,80

Coordinado por

KOBENHAVNS UNIVERSITET
Denmark

Objetivo

This project aims at reconstructing the evolution of human oral health in Northern Europeans, from the late Mesolithic to modern days, by genomics and proteomics investigation of: human ancient microbiota, human immune response proteins, and food remains preserved in mineralised dental plaque (archaeological dental calculus).
The human microbiota is essential to maintain health, but capable of eliciting disease. Ecological shifts and imbalances (dysbiosis) of the oral microbiota can significantly impact health and cause severe forms of oral pathologies resulting in systemic disease at different body sites. Taxonomic reconstruction of ancient microbiomes and identification of which genes have been gained or lost in their genomes in connection with major lifestyle changes in human history will contribute to the development of more effective preventive and therapeutic approaches for this disease.
The two main research objectives are:
• Reconstruction of the evolution of the oral microbiome composition over the last 8000 years, and
• Characterisation of the associated human immune responses.
Genomic and proteomics biomolecular evidence will be retrieved from archaeological human dental calculus: a rich, but so far mostly neglected, source of ancient oral biomolecules. Ancient DNA and proteins extracted from archaeological dental calculus will be analysed using state-of-the-art proteomics and metagenomics approaches at the Centre for GeoGenetics, by high-resolution mass spectrometry and high-throughput DNA sequencing-by-synthesis methods. CGG recently led a high-profile study demonstrating the potential of this approach and will further develop this concept using approximately 80 Northern European samples from a wide chronological range. The Researcher’s skills in computational proteomics will be beneficial to ancient proteomics analysis, and his skills extended to the field of metagenomics.

Ámbito científico (EuroSciVoc)

• ciencias naturales › ciencias biológicas › bioquímica › biomoléculas › proteínas › proteómica
• ciencias naturales › ciencias biológicas › genética › ADN
• ciencias naturales › ciencias químicas › química analítica › espectrometría de masas
• ciencias naturales › ciencias biológicas › genética › genoma
• ciencias naturales › ciencias biológicas › microbiología

Palabras clave

• Ancient DNA
• ancient proteins
• ancient metagenomics
• oral microbiome
• mass spectrometry

Programa(s)

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions PROGRAMA PRINCIPAL
  
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
  

Tema(s)

• MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF)
  

Régimen de financiación

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

Convocatoria de propuestas

H2020-MSCA-IF-2014

Coordinador

KOBENHAVNS UNIVERSITET

• Aportación neta de la UEn: € 200 194,80
• Dirección: NORREGADE 10 ; 1165 KOBENHAVN ; Dinamarca
• Región: Danmark › Hovedstaden › Byen København
• Tipo de actividad: Higher or Secondary Education Establishments
• Coste total: € 200 194,80