CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.
Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .
Livrables
Test of uptake of particles into endothelial cells and into human PBMCs by microscopy and MRI. MRI will be done after cells have been incubated with different dilutions of the NPs and embedded in agarose. Contrast changes due to switching of B0 in phantoms and in cell cultures after cellular uptake will be studied and documented.
Quantum-mechanical model 1 (s’ouvre dans une nouvelle fenêtre)An NQR-modeling approach which has been developed for solids will be extended towards different motional scenarios to account for rotational, translational and internal dynamics as well as exchange processes. Existing numerical tools will be used for initial simulations but the software will be continuously developed to account for the complex relaxation scenario. Optimisation is likely to be required as the computational complexity increases significantly for high spin (9/2) nuclei. Where necessary, the quantum-mechanical models will be combined with molecular dynamics simulations.
Scientific papers of reporting period 2 (s’ouvre dans une nouvelle fenêtre)8 Scientific papers planned on theoretical modelling, synthesis of NQR-CR compounds and their characterisation as well as on toxicology and imaging results. Papers on the proof of concept.
Report on publications and events (s’ouvre dans une nouvelle fenêtre)Report on publications (journal and book articles, patents etc. as well as events such as workshops and mini-symposia on conferences.
Scientific papers of reporting period 1 (s’ouvre dans une nouvelle fenêtre)3 Scientific papers planned on theoretical modelling, synthesis of NQR-CR compounds and their characterisation.
Cytotoxicity, uptake, inflammation (s’ouvre dans une nouvelle fenêtre)Cell cultures for MRI experiments will be prepared. Absence of biological contamination, sufficient cellular uptake and lack of adverse cellular effects will be shown with endothelial cells and blood cells. Contamination will be detected by subjecting the NPs to limulus amoebocyte lysate assay to test endotoxin levels Cytotoxicity and potential influence on the immune system will be studied and documented.
Tuned NPs characterised (s’ouvre dans une nouvelle fenêtre)After optimisation of the prototype particle system (size, chemistry and hydrophilicity the NQR properties will be tuned in order to fulfil the 3T conditions. Thus a fine tuning of the particle size, shape and slight chemical modifications may be required.
Modified MRI scanner characterised (s’ouvre dans une nouvelle fenêtre)"The existing clinical 3T scanner will be equipped with a coil insert for shifting B0 so as to change the NQR-CR (custom manufacturing by ""Resonance Research""). The RF coils used will be dedicated animal coils. Existing pulse sequences will be modified to control the coil insert and optimised for high SNR. The complete MRI setup will be validated and characterized with dedicated MRI phantoms. "
Extended quantum-mechanical model (s’ouvre dans une nouvelle fenêtre)Theoretical models and simulations for systems containing QN and paramagnetic ions, different kinds of QN, will be developed to explore the possibilities offered by multi-quantum effects. Although a comprehensive model is necessary for predicting in detail the contrast properties of the CA, we will also develop simplified, phenomenological ‗master rules‘ relating the shape of the NQR-CR peaks with structural and dynamical parameters. Due to their complexity the developed models will be thoroughly tested against experiments
Relaxometry data base tuned NPs (s’ouvre dans une nouvelle fenêtre)1H NMR Field Cycling studies for the final, fine-tuned compounds synthetised by WPs 3 and 4 will be carried out. The results will again be documented and stored in a database.
ZF-NQRS database (s’ouvre dans une nouvelle fenêtre)Zero field NQRS of promising prototype compounds from WP3 will be carried out. From the spectra the resonance frequencies and the facor eta will be deduced and the quantum-chemical computations in WP3 will be validated. The relative strength of the NQR peaks and the NQR relaxation times will be characterised to study the magnitude of the effects in different compounds. All data will be stored in a database.
Relaxometry database prototyping (s’ouvre dans une nouvelle fenêtre)1H NMR Field Cycling studies for selected prototype compounds synthesised by WPs 3 and 4 will be carried out. Preliminary measurements will be carried out in parallel to the theoretical simulations. The preliminary studies will be followed by detailed experimental investigations of specific systems selected on the basis of simulations to confirm or negate their potential as CA. All experimental results will be documented and stored in a database.
Dextran based NPs characterised (s’ouvre dans une nouvelle fenêtre)Immobilisation of the most promising candidates from WP3 (TUG) in terms of NQR frequencies onto dextranes differing in molecular weight and the degree of branching. The immobilisation will be performed by reacting the Bi compounds bearing a functional spacer group (e.g. carboxylic groups) with the polymers. For example, carbodiimide chemistry is a well-known procedure for coupling OH with COOH groups in a very efficient way, and several literature procedures are known. Purification (e.g. dialysis) of the polymers and nanoprecipitation under sonication conditions to create nano- and micro-particles with defined sizes according to the guidelines WP2 from UWM and UU. The sizes will be varied over two orders of magnitudes in order to explore the influence on relaxivity. (Task 4.2, UM, TUG, UWM) Characterisation of the NPs by state of the art techniques (Infrared spectroscopy, SWAXS, TEM, dynamic light scattering, zeta potential, ZF-NQRS, fast field cycling relaxometry).
NQR-CR polymers characterised (s’ouvre dans une nouvelle fenêtre)Immobilisation of the most promising candidates from WP3 in terms of NQR frequencies onto PEG differing in molecular weight and the degree of branching. The immobilisation will be performed by reacting the Bi compounds bearing a functional spacer group (e.g. carboxylic groups) with the polymers. For example, carbodiimide chemistry is a well-known procedure for coupling OH with COOH groups in a very efficient way, and several literature procedures are known. Purification (e.g. dialysis) of the polymers and nanoprecipitation under sonication conditions to create nano- and micro-particles with defined sizes according to the guidelines WP2 from UWM and UU. The sizes will be varied over two orders of magnitudes in order to explore the influence on relaxivity. Characterisation of the NPs by state of the art techniques (Infrared spectroscopy, SWAXS, TEM, dynamic light scattering, zeta potential, ZF-NQRS, fast field cycling relaxometry).
Detailed rules for data management, enabling data exchange by supplying appropriate channels and assurance of data security (server management).
Design and implementation of a webpage for communicating general information and results, providing open access to data and for facilitating networking activities.
Publications
Auteurs:
Hermann Scharfetter
Publié dans:
Journal of Magnetic Resonance, Numéro 271, 2016, Page(s) 90-98, ISSN 1090-7807
Éditeur:
Academic Press
DOI:
10.1016/j.jmr.2016.08.008
Auteurs:
Hermann Scharfetter, Markus Bödenler, Dominik Narnhofer
Publié dans:
Journal of Magnetic Resonance, Numéro 286, 2018, Page(s) 148-157, ISSN 1090-7807
Éditeur:
Academic Press
DOI:
10.1016/j.jmr.2017.12.004
Auteurs:
Markus Bödenler, Martina Basini, Maria Francesca Casula, Evrim Umut, Christian Gösweiner, Andreas Petrovic, Danuta Kruk, Hermann Scharfetter
Publié dans:
Journal of Magnetic Resonance, Numéro 290, 2018, Page(s) 68-75, ISSN 1090-7807
Éditeur:
Academic Press
DOI:
10.1016/j.jmr.2018.03.010
Auteurs:
D. Kruk, E. Umut, E. Masiewicz, C. Sampl, R. Fischer, S. Spirk, C. Goesweiner, H. Scharfetter
Publié dans:
Physical Chemistry Chemical Physics, Numéro 20/18, 2018, Page(s) 12710-12718, ISSN 1463-9076
Éditeur:
Royal Society of Chemistry
DOI:
10.1039/c8cp00993g
Auteurs:
Danuta Kruk, Elzbieta Masiewicz, Evrim Umut, Hermann Scharfetter
Publié dans:
Molecular Physics, 2018, Page(s) 1-6, ISSN 0026-8976
Éditeur:
Taylor & Francis
DOI:
10.1080/00268976.2018.1513175
Auteurs:
Christian Gösweiner, Perttu Lantto, Roland Fischer, Carina Sampl, Evrim Umut, Per-Olof Westlund, Danuta Kruk, Markus Bödenler, Stefan Spirk, Andreas Petrovič, Hermann Scharfetter
Publié dans:
Physical Review X, Numéro 8/2, 2018, ISSN 2160-3308
Éditeur:
American Physical Society
DOI:
10.1103/PhysRevX.8.021076
Auteurs:
Danuta Kruk, Christian Goesweiner, Elzbieta Masiewicz, Evrim Umut, Carina Sampl, Hermann Scharfetter
Publié dans:
Physical Chemistry Chemical Physics, Numéro 20/36, 2018, Page(s) 23414-23423, ISSN 1463-9076
Éditeur:
Royal Society of Chemistry
DOI:
10.1039/C8CP03848A
Auteurs:
Danuta Kruk, Evrim Umut, Elzbieta Masiewicz, Petr Hermann, Hermann Scharfetter
Publié dans:
Molecular Physics, 2018, Page(s) 1-8, ISSN 0026-8976
Éditeur:
Taylor & Francis
DOI:
10.1080/00268976.2018.1517907
Auteurs:
Christian Gösweiner, Danuta Kruk, Evrim Umut, Elzbieta Masiewicz, Markus Bödenler, Hermann Scharfetter
Publié dans:
Molecular Physics, 2018, Page(s) 1-11, ISSN 0026-8976
Éditeur:
Taylor & Francis
DOI:
10.1080/00268976.2018.1519201
Auteurs:
Hermann Scharfetter, Christian Gösweiner, Paul Josef Krassnig, Carina Sampl, Martin Thonhofer, Roland Fischer, Stefan Spirk, Rupert Kargl, Karin Stana-Kleinschek, Evrim Umut, Danuta Kruk
Publié dans:
Molecular Physics, 2018, Page(s) 1-9, ISSN 0026-8976
Éditeur:
Taylor & Francis
DOI:
10.1080/00268976.2018.1511869
Auteurs:
Markus Bödenler, Ludovic de Rochefort, P. James Ross, Nicolas Chanet, Geneviève Guillot, Gareth R. Davies, Christian Gösweiner, Hermann Scharfetter, David J. Lurie, Lionel M. Broche
Publié dans:
Molecular Physics, Numéro 117/7-8, 2018, Page(s) 832-848, ISSN 0026-8976
Éditeur:
Taylor & Francis
DOI:
10.1080/00268976.2018.1557349
Auteurs:
Danuta Kruk, Elzbieta Masiewicz, Evrim Umut, Martin Schlögl, Roland Fischer, Hermann Scharfetter
Publié dans:
Molecular Physics, Numéro 117/7-8, 2018, Page(s) 944-951, ISSN 0026-8976
Éditeur:
Taylor & Francis
DOI:
10.1080/00268976.2018.1552798
Auteurs:
Mojca Božič, Thomas Elschner, Doris Tkaučič, Matej Bračič, Silvo Hribernik, Karin Stana Kleinschek, Rupert Kargl
Publié dans:
Cellulose, Numéro 25/12, 2018, Page(s) 6901-6922, ISSN 0969-0239
Éditeur:
Blackie Academic & Professional
DOI:
10.1007/s10570-018-2062-2
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