Periodic Reporting for period 1 - NeuroNanoChip (A Nano-electrode chip for high-resolution/high-throughput cellular assays in cultured neurons including synaptic tranmission)
Période du rapport: 2015-07-01 au 2016-12-31
We established a new approach to electrically record synaptic transmission in many single neurons in parallel, with high-resolution, using nano-electrodes on a chip. We showed that we can grow nerve cells in a regular grid, forming dense networks on a silicon chip. Furthermore, we developed nano-scale versions of the conventional patch clamp electrodes for synaptic recordings on a chip and novel approaches to obtain high resistance seals to the cell membrane. Massive parallel electrical recording with single cell resolution will not only facilitate and standardize cellular assays using real, synaptic neurons, but also upscale throughput, probably by at least 100 fold, which will make synaptic transmission accessible for applications in the pharmaceutical industry and personalized medicine.