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Production and characterization of endocrine cells derived from human pancreas organoids for the cell-based therapy of type 1 diabetes

Objective

Self-renew and multilineage potential characterize stem cells. We have recently described that pancreas progenitor cells extracted from adult donors can be expanded long-term in vitro into 3D structures, which we have termed organoids. Pancreas organoids reproduce in vitro all the features of pancreas ductal epithelia, and have a limitless expansion potential. Thus, pancreas organoids promise to boost cell therapy of type 1 diabetes. We have recently observed that progenitor cells organoids preserve their genetic stability over a long time in culture. That represents an advantage, when compared to iPS or hES derived approaches, where genetic instability raises concerns for their future therapeutic applications. While progenitor organoids are promising for the future of cell therapy, bringing stem cell-based therapies to patients requires a reliable characterization (“knowing what the cells do and how they do it”, i.e. a phenotypic and molecular biology characterization), chemically well-defined culture media, and the capacity of mass-production under GLP/GMP conditions.
The LSFM4LIFE consortium aims to the mass production of pancreas organoids for the cellular therapy of type 1 diabetes. The goals of the project are: (1) optimize growth and differentiation of human pancreas stem-cell organoids by employing phenotypic and molecular high-throughput screening (2) standardize the growth and differentiation of the organoids under well-defined biochemical conditions, and (3) achieve GLP/GMP-production of the human organoids for preclinical studies and phase I clinical studies. The close collaboration in the consortium between academic researchers and industry, as well as its cross-disciplinary composition, are essential to realize the goals of the project. The work packages of the project will have a technological impact in the form of patents and first market replication.

Call for proposal

H2020-PHC-2014-2015

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Sub call

H2020-PHC-2015-two-stage

Coordinator

JOHANN WOLFGANG GOETHE-UNIVERSITAET FRANKFURT AM MAIN
Net EU contribution
€ 1 068 886,25
Address
Theodor W Adorno Platz 1
60323 Frankfurt Am Main
Germany

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Region
Baden-Württemberg Stuttgart Stuttgart, Stadtkreis
Activity type
Higher or Secondary Education Establishments
Other funding
€ 0,00

Participants (7)

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
United Kingdom
Net EU contribution
€ 1 432 990,00
Address
Trinity Lane The Old Schools
CB2 1TN Cambridge

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Region
East of England East Anglia Cambridgeshire CC
Activity type
Higher or Secondary Education Establishments
Other funding
€ 0,00
CELLENDES GMBH
Germany
Net EU contribution
€ 589 233,75
Address
Markwiesenstr 55
72770 Reutlingen

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Baden-Württemberg Tübingen Reutlingen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Other funding
€ 0,00
FONDAZIONE IRCCS CA' GRANDA - OSPEDALE MAGGIORE POLICLINICO
Italy
Net EU contribution
€ 895 000,00
Address
Via Francesco Sforza 28
20122 Milano

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Region
Nord-Ovest Lombardia Milano
Activity type
Other
Other funding
€ 0,00
INSPHERO AG
Switzerland
Net EU contribution
€ 0,00
Address
Wagistrasse 27
8952 Schlieren

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Schweiz/Suisse/Svizzera Zürich Zürich
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Other funding
€ 453 875,00
LONZA NETHERLANDS BV
Netherlands
Net EU contribution
€ 463 950,00
Address
Oxfordlaan 70
6229 EV Maastricht

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Region
Limburg (NL) Zuid-Limburg
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Other funding
€ 0,00
SPARKS & CO

Participation ended

France
Net EU contribution
€ 133 153,46
Address
66 Avenue Des Champs Elysees Espace 41
75008 Paris
SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Ile-de-France Ile-de-France Paris
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Other funding
€ 0,00
PROSCIENCIA BERATUNGS-GMBH
Germany
Net EU contribution
€ 83 186,54
Address
Auf Der Heide 11
23558 Luebeck

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Schleswig-Holstein Schleswig-Holstein Lübeck, Kreisfreie Stadt
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Other funding
€ 0,00