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Tools and TECHNOlogies for Breakthrough in hEArt Therapies

Deliverables

Website launched, central data base accessible to all partners

Website launched, central data base accessible to all partners

Final dissemination activity on project results

Final dissemination activity on project results

Draft and disseminate press release on project initiation

Draft and disseminate press release on project initiation

Final report on efficacy and retention of ―Second stage μ-tissues‖ versus ―second stage μ-tissues plus MSC co-injected‖ either with or without simultaneous injection of supportive compounds

Final report on efficacy and retention of ―Second stage μ-tissues‖ versus ―second stage μ-tissues plus MSC co-injected‖ either with or without simultaneous injection of supportive compounds

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Publications

Scalable Cardiac Differentiation of Pluripotent Stem Cells Using Specific Growth Factors and Small Molecules

Author(s): Henning Kempf, Robert Zweigerdt
Published in: Engineering and Application of Pluripotent Stem Cells, Issue 163, 2018, Page(s) 39-69
DOI: 10.1007/10_2017_30

Engineering and applicaions of pluripotent stem cells - Acquired genetic and epigenetic varaition in human pluripotent stem cells

Author(s): Kyriakides, O., Halliwell, J., Andrews, P.
Published in: 2018

Bioreactor-based mass production of human iPSC-derived macrophages enables immunotherapies against bacterial airway infections

Author(s): Mania Ackermann, Henning Kempf, Miriam Hetzel, Christina Hesse, Anna Rafiei Hashtchin, Kerstin Brinkert, Juliane Wilhelmine Schott, Kathrin Haake, Mark Philipp Kühnel, Silke Glage, Constanca Figueiredo, Danny Jonigk, Katherina Sewald, Axel Schambach, Sabine Wronski, Thomas Moritz, Ulrich Martin, Robert Zweigerdt, Antje Munder, Nico Lachmann
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
DOI: 10.1038/s41467-018-07570-7

Paracrine mechanisms in early differentiation of human pluripotent stem cells: Insights from a mathematical model

Author(s): Erika Gaspari, Annika Franke, Diana Robles-Diaz, Robert Zweigerdt, Ingo Roeder, Thomas Zerjatke, Henning Kempf
Published in: Stem Cell Research, Issue 32, 2018, Page(s) 1-7, ISSN 1873-5061
DOI: 10.1016/j.scr.2018.07.025

A Cardiac Cell Outgrowth Assay for Evaluating Drug Compounds Using a Cardiac Spheroid-on-a-Chip Device

Author(s): Jonas Christoffersson, Florian Meier, Henning Kempf, Kristin Schwanke, Michelle Coffee, Mario Beilmann, Robert Zweigerdt, Carl-Fredrik Mandenius
Published in: Bioengineering, Issue 5/2, 2018, Page(s) 36, ISSN 2306-5354
DOI: 10.3390/bioengineering5020036

Anti-androgenic therapy with finasteride improves cardiac function, attenuates remodeling and reverts pathologic gene-expression after myocardial infarction in mice

Author(s): Natali Froese, Honghui Wang, Carolin Zwadlo, Yong Wang, Andrea Grund, Anna Gigina, Melanie Hofmann, Katja Kilian, Gesine Scharf, Mortimer Korf-Klingebiel, Anna Melchert, Maria Elena Ricci Signorini, Caroline Halloin, Robert Zweigerdt, Ulrich Martin, Ina Gruh, Kai C. Wollert, Robert Geffers, Johann Bauersachs, Joerg Heineke
Published in: Journal of Molecular and Cellular Cardiology, Issue 122, 2018, Page(s) 114-124, ISSN 0022-2828
DOI: 10.1016/j.yjmcc.2018.08.011

Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies

Author(s): Gesa-Maria Gieseler, Kimia Ekramzadeh, Volker Nölle, Svitlana Malysheva, Henning Kempf, Sascha Beutel, Robert Zweigerdt, Ulrich Martin, Ursula Rinas, Thomas Scheper, Iliyana Pepelanova
Published in: Biotechnology Reports, Issue 18, 2018, Page(s) e00249, ISSN 2215-017X
DOI: 10.1016/j.btre.2018.e00249

Laser bioprinting of human induced pluripotent stem cells—the effect of printing and biomaterials on cell survival, pluripotency, and differentiation

Author(s): Lothar Koch, Andrea Deiwick, Annika Franke, Kristin Schwanke, Axel Haverich, Robert Zweigerdt, Boris Chichkov
Published in: Biofabrication, Issue 10/3, 2018, Page(s) 035005, ISSN 1758-5090
DOI: 10.1088/1758-5090/aab981

Expansion of functional personalized cells with specific transgene combinations

Author(s): Christoph Lipps, Franziska Klein, Tom Wahlicht, Virginia Seiffert, Milada Butueva, Jeannette Zauers, Theresa Truschel, Martin Luckner, Mario Köster, Roderick MacLeod, Jörn Pezoldt, Jochen Hühn, Qinggong Yuan, Peter Paul Müller, Henning Kempf, Robert Zweigerdt, Oliver Dittrich-Breiholz, Thomas Pufe, Rainer Beckmann, Wolf Drescher, Jose Riancho, Carolina Sañudo, Thomas Korff, Bertram Opalka, Ve
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
DOI: 10.1038/s41467-018-03408-4

Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture

Author(s): Ruth Olmer, Lena Engels, Abdulai Usman, Sandra Menke, Muhammad Nasir Hayat Malik, Frank Pessler, Gudrun Göhring, Dorothee Bornhorst, Svenja Bolten, Salim Abdelilah-Seyfried, Thomas Scheper, Henning Kempf, Robert Zweigerdt, Ulrich Martin
Published in: Stem Cell Reports, Issue 10/5, 2018, Page(s) 1657-1672, ISSN 2213-6711
DOI: 10.1016/j.stemcr.2018.03.017

Differences in Contractile Function of Myofibrils within Human Embryonic Stem Cell-Derived Cardiomyocytes vs. Adult Ventricular Myofibrils Are Related to Distinct Sarcomeric Protein Isoforms

Author(s): Bogdan Iorga, Kristin Schwanke, Natalie Weber, Meike Wendland, Stephan Greten, Birgit Piep, Cristobal G. dos Remedios, Ulrich Martin, Robert Zweigerdt, Theresia Kraft, Bernhard Brenner
Published in: Frontiers in Physiology, Issue 8, 2018, ISSN 1664-042X
DOI: 10.3389/fphys.2017.01111

Differential Expression of Cholinergic System Components in Human Induced Pluripotent Stem Cells, Bone Marrow-Derived Multipotent Stromal Cells, and Induced Pluripotent Stem Cell-Derived Multipotent Stromal Cells

Author(s): Ramona Weist, Thilo Flörkemeier, Yvonne Roger, Sandra Noack, Annika Franke, Kristin Schwanke, Robert Zweigerdt, Ulrich Martin, Elmar Willbold, Andrea Hoffmann
Published in: Stem Cells and Development, Issue 27/3, 2018, Page(s) 166-183, ISSN 1547-3287
DOI: 10.1089/scd.2017.0162

Assessment of established techniques to determine developmental and malignant potential of human pluripotent stem cells

Author(s): Allison TF, Andrews PW, Avior Y, Barbaric I, Benvenisty N, Bock C, Brehm J, Brüstle O, Damjanov I, Elefanty A, Felkner D, Gokhale PJ, Halbritter F, Healy LE, Hu TX, Knowles BB, Loring JF, Ludwig TE, Mayberry R, Micallef S, Mohamed JS, Müller FJ, Mummery CL, Nakatsuji N, Ng ES, Oh SKW, O'Shea O, Pera MF, Reubinoff B, Robson P, Rossant J, Schuldt BM, Solter D, Sourris K, Stacey G, Stanley EG, Suem
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
DOI: 10.1038/s41467-018-04011-3

Large-scale production of megakaryocytes in microcarrier-supported stirred suspension bioreactors

Author(s): Dorothee Eicke, Anja Baigger, Kai Schulze, Sharissa L. Latham, Caroline Halloin, Robert Zweigerdt, Carlos A. Guzman, Rainer Blasczyk, Constança Figueiredo
Published in: Scientific Reports, Issue 8/1, 2018, ISSN 2045-2322
DOI: 10.1038/s41598-018-28459-x

Inflammatory Responses and Barrier Function of Endothelial Cells Derived from Human Induced Pluripotent Stem Cells

Author(s): Oleh V. Halaidych, Christian Freund, Francijna van den Hil, Daniela C.F. Salvatori, Mara Riminucci, Christine L. Mummery, Valeria V. Orlova
Published in: Stem Cell Reports, Issue 10/5, 2018, Page(s) 1642-1656, ISSN 2213-6711
DOI: 10.1016/j.stemcr.2018.03.012

Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells

Author(s): Juan Antonio Guadix, Valeria V. Orlova, Elisa Giacomelli, Milena Bellin, Marcelo C. Ribeiro, Christine L. Mummery, José M. Pérez-Pomares, Robert Passier
Published in: Stem Cell Reports, Issue 9/6, 2017, Page(s) 1754-1764, ISSN 2213-6711
DOI: 10.1016/j.stemcr.2017.10.023

Modelling inherited cardiac disease using human induced pluripotent stem cell-derived cardiomyocytes: progress, pitfalls, and potential

Author(s): Alain van Mil, Geerthe Margriet Balk, Klaus Neef, Jan Willem Buikema, Folkert W Asselbergs, Sean M Wu, Pieter A Doevendans, Joost P G Sluijter
Published in: Cardiovascular Research, 2018, ISSN 0008-6363
DOI: 10.1093/cvr/cvy208

ESC Working Group on Cellular Biology of the Heart: position paper for Cardiovascular Research: tissue engineering strategies combined with cell therapies for cardiac repair in ischaemic heart disease and heart failure

Author(s): Rosalinda Madonna, Linda W Van Laake, Hans Erik Botker, Sean M Davidson, Raffaele De Caterina, Felix B Engel, Thomas Eschenhagen, Francesco Fernandez-Aviles, Derek J Hausenloy, Jean-Sebastien Hulot, Sandrine Lecour, Jonathan Leor, Philippe Menasché, Maurizio Pesce, Cinzia Perrino, Fabrice Prunier, Sophie Van Linthout, Kirsti Ytrehus, Wolfram-Hubertus Zimmermann, Peter Ferdinandy, Joost P G Sluijt
Published in: Cardiovascular Research, 2019, ISSN 0008-6363
DOI: 10.1093/cvr/cvz010

Evaluation of modified Interferon alpha mRNA constructs for the treatment of non-melanoma skin cancer

Author(s): Sarah Hochmann, Michaela Mittermeir, Radmila Santic, Frieder Koszik, Lanay Griessner, Alina Sarah Sonderegger, Thomas Hoffmann, Elisabeth Russe, Sandra Scheiblhofer, Richard Weiss, Markus Mandler, Achim Schneeberger, Dirk Strunk
Published in: Scientific Reports, Issue 8/1, 2018, ISSN 2045-2322
DOI: 10.1038/s41598-018-31061-w

Selection of Tissue Factor-Deficient Cell Transplants as a Novel Strategy for Improving Hemocompatibility of Human Bone Marrow Stromal Cells

Author(s): Michaela Oeller, Sandra Laner-Plamberger, Sarah Hochmann, Nina Ketterl, Martina Feichtner, Gabriele Brachtl, Anna Hochreiter, Cornelia Scharler, Lara Bieler, Pasquale Romanelli, Sebastien Couillard-Despres, Elisabeth Russe, Katharina Schallmoser, Dirk Strunk
Published in: Theranostics, Issue 8/5, 2018, Page(s) 1421-1434, ISSN 1838-7640
DOI: 10.7150/thno.21906

Advanced in vitro models of vascular biology: Human induced pluripotent stem cells and organ-on-chip technology

Author(s): Amy Cochrane, Hugo J. Albers, Robert Passier, Christine L. Mummery, Albert van den Berg, Valeria V. Orlova, Andries D. van der Meer
Published in: Advanced Drug Delivery Reviews, Issue 140, 2019, Page(s) 68-77, ISSN 0169-409X
DOI: 10.1016/j.addr.2018.06.007

Quantifying Ca2+ signaling and contraction in vascular pericytes and smooth muscle cells

Author(s): Oleh V. Halaidych, Christine L. Mummery, Valeria V. Orlova
Published in: Biochemical and Biophysical Research Communications, Issue 513/1, 2019, Page(s) 112-118, ISSN 0006-291X
DOI: 10.1016/j.bbrc.2019.03.143

Closing the Mitochondrial Permeability Transition Pore in hiPSC-Derived Endothelial Cells Induces Glycocalyx Formation and Functional Maturation

Author(s): Gesa L. Tiemeier, Gangqi Wang, Sébastien J. Dumas, Wendy M.P.J. Sol, M. Cristina Avramut, Tobias Karakach, Valeria V. Orlova, Cathelijne W. van den Berg, Christine L. Mummery, Peter Carmeliet, Bernard M. van den Berg, Ton J. Rabelink
Published in: Stem Cell Reports, Issue 13/5, 2019, Page(s) 803-816, ISSN 2213-6711
DOI: 10.1016/j.stemcr.2019.10.005

Quantitative Analysis of Intracellular Ca2+ Release and Contraction in hiPSC-Derived Vascular Smooth Muscle Cells

Author(s): Oleh V. Halaidych, Amy Cochrane, Francijna E. van den Hil, Christine L. Mummery, Valeria V. Orlova
Published in: Stem Cell Reports, Issue 12/4, 2019, Page(s) 647-656, ISSN 2213-6711
DOI: 10.1016/j.stemcr.2019.02.003

Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)

Author(s): Oleh V. Halaidych, Francijna van den Hil, Christine L. Mummery, Valeria V. Orlova
Published in: Journal of Visualized Experiments, Issue 141, 2018, ISSN 1940-087X
DOI: 10.3791/58678

Chemically-Defined, Xeno-Free, Scalable Production of hPSC-Derived Definitive Endoderm Aggregates with Multi-Lineage Differentiation Potential

Author(s): Anais Sahabian, Malte Sgodda, Ortwin Naujok, Rabea Dettmer, Julia Dahlmann, Felix Manstein, Tobias Cantz, Robert Zweigerdt, Ulrich Martin, Ruth Olmer
Published in: Cells, Issue 8/12, 2019, Page(s) 1571, ISSN 2073-4409
DOI: 10.3390/cells8121571

A COUP-TFII Human Embryonic Stem Cell Reporter Line to Identify and Select Atrial Cardiomyocytes

Author(s): Verena Schwach, Arie O. Verkerk, Mervyn Mol, Jantine J. Monshouwer-Kloots, Harsha D. Devalla, Valeria V. Orlova, Konstantinos Anastassiadis, Christine L. Mummery, Richard P. Davis, Robert Passier
Published in: Stem Cell Reports, Issue 9/6, 2017, Page(s) 1765-1779, ISSN 2213-6711
DOI: 10.1016/j.stemcr.2017.10.024

Scalable microphysiological system to model three-dimensional blood vessels

Author(s): Mees N. S. de Graaf, Amy Cochrane, Francijna E. van den Hil, Wesley Buijsman, Andries D. van der Meer, Albert van den Berg, Christine L. Mummery, Valeria V. Orlova
Published in: APL Bioengineering, Issue 3/2, 2019, Page(s) 026105, ISSN 2473-2877
DOI: 10.1063/1.5090986

GMP-compatible manufacturing of three iPS cell lines from human peripheral blood

Author(s): Alexandra Haase, Wolfgang Glienke, Lena Engels, Gudrun Göhring, Ruth Esser, Lubomir Arseniev, Ulrich Martin
Published in: Stem Cell Research, Issue 35, 2019, Page(s) 101394, ISSN 1873-5061
DOI: 10.1016/j.scr.2019.101394

Differentiation and Functional Comparison of Monocytes and Macrophages from hiPSCs with Peripheral Blood Derivatives

Author(s): Xu Cao, Gopala K. Yakala, Francijna E. van den Hil, Amy Cochrane, Christine L. Mummery, Valeria V. Orlova
Published in: Stem Cell Reports, Issue 12/6, 2019, Page(s) 1282-1297, ISSN 2213-6711
DOI: 10.1016/j.stemcr.2019.05.003

Three-dimensional cardiac microtissues composed of cardiomyocytes and endothelial cells co-differentiated from human pluripotent stem cells

Author(s): Elisa Giacomelli, Milena Bellin, Luca Sala, Berend J. van Meer, Leon G. J. Tertoolen, Valeria V. Orlova, Christine L. Mummery
Published in: Development, Issue monthly, 2017, Page(s) dev.143438, ISSN 0950-1991
DOI: 10.1242/dev.143438

Sensitivity of human pluripotent stem cells to insulin precipitation induced by peristaltic pump-based medium circulation: considerations on process development

Author(s): Diana Massai, Emiliano Bolesani, Diana Robles Diaz, Christina Kropp, Henning Kempf, Caroline Halloin, Ulrich Martin, Tudor Braniste, Giuseppe Isu, Vanessa Harms, Umberto Morbiducci, Gerald Dräger, Robert Zweigerdt
Published in: Scientific Reports, Issue 7/1, 2017, ISSN 2045-2322
DOI: 10.1038/s41598-017-04158-x

Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells

Author(s): Henning Kempf, Ruth Olmer, Alexandra Haase, Annika Franke, Emiliano Bolesani, Kristin Schwanke, Diana Robles-Diaz, Michelle Coffee, Gudrun Göhring, Gerald Dräger, Oliver Pötz, Thomas Joos, Erik Martinez-Hackert, Axel Haverich, Falk F. R. Buettner, Ulrich Martin, Robert Zweigerdt
Published in: Nature Communications, Issue 7, 2016, Page(s) 13602, ISSN 2041-1723
DOI: 10.1038/ncomms13602

Proteomic Analysis of Human Pluripotent Stem Cell Cardiomyogenesis Revealed Altered Expression of Metabolic Enzymes and PDLIM5 Isoforms

Author(s): Sarah A. Konze, Sebastian Werneburg, Astrid Oberbeck, Ruth Olmer, Henning Kempf, Monica Jara-Avaca, Andreas Pich, Robert Zweigerdt, Falk F. R. Buettner
Published in: Journal of Proteome Research, Issue 16/3, 2017, Page(s) 1133-1149, ISSN 1535-3893
DOI: 10.1021/acs.jproteome.6b00534

Stromal Cells Act as Guardians for Endothelial Progenitors by Reducing Their Immunogenicity After Co-Transplantation

Author(s): Naima Souidi, Meaghan Stolk, Juliane Rudeck, Dirk Strunk, Katharina Schallmoser, Hans-Dieter Volk, Martina Seifert
Published in: STEM CELLS, Issue 35/5, 2017, Page(s) 1233-1245, ISSN 1066-5099
DOI: 10.1002/stem.2573

Impact of Feeding Strategies on the Scalable Expansion of Human Pluripotent Stem Cells in Single-Use Stirred Tank Bioreactors

Author(s): Christina Kropp, Henning Kempf, Caroline Halloin, Diana Robles-Diaz, Annika Franke, Thomas Scheper, Katharina Kinast, Thomas Knorpp, Thomas O. Joos, Axel Haverich, Ulrich Martin, Robert Zweigerdt, Ruth Olmer
Published in: STEM CELLS Translational Medicine, Issue 5/10, 2016, Page(s) 1289-1301, ISSN 2157-6564
DOI: 10.5966/sctm.2015-0253

A humanized bone marrow ossicle xenotransplantation model enables improved engraftment of healthy and leukemic human hematopoietic cells

Author(s): Andreas Reinisch, Daniel Thomas, M Ryan Corces, Xiaohua Zhang, Dita Gratzinger, Wan-Jen Hong, Katharina Schallmoser, Dirk Strunk, Ravindra Majeti
Published in: Nature Medicine, Issue 22/7, 2016, Page(s) 812-821, ISSN 1078-8956
DOI: 10.1038/nm.4103

Progress and challenges in large-scale expansion of human pluripotent stem cells

Author(s): Christina Kropp, Diana Massai, Robert Zweigerdt
Published in: Process Biochemistry, 2016, ISSN 1359-5113
DOI: 10.1016/j.procbio.2016.09.032

DNA methylation heterogeneity defines a disease spectrum in Ewing sarcoma

Author(s): Nathan C Sheffield, Gaelle Pierron, Johanna Klughammer, Paul Datlinger, Andreas Schönegger, Michael Schuster, Johanna Hadler, Didier Surdez, Delphine Guillemot, Eve Lapouble, Paul Freneaux, Jacqueline Champigneulle, Raymonde Bouvier, Diana Walder, Ingeborg M Ambros, Caroline Hutter, Eva Sorz, Ana T Amaral, Enrique de Álava, Katharina Schallmoser, Dirk Strunk, Beate Rinner, Bernadette Liegl-Atzwa
Published in: Nature Medicine, Issue 23/3, 2017, Page(s) 386-395, ISSN 1078-8956
DOI: 10.1038/nm.4273

EBIO Does Not Induce Cardiomyogenesis in Human Pluripotent Stem Cells but Modulates Cardiac Subtype Enrichment by Lineage-Selective Survival

Author(s): Monica Jara-Avaca, Henning Kempf, Michael Rückert, Diana Robles-Diaz, Annika Franke, Jeanne de la Roche, Martin Fischer, Daniela Malan, Philipp Sasse, Wladimir Solodenko, Gerald Dräger, Andreas Kirschning, Ulrich Martin, Robert Zweigerdt
Published in: Stem Cell Reports, Issue 8/2, 2017, Page(s) 305-317, ISSN 2213-6711
DOI: 10.1016/j.stemcr.2016.12.012

Listen to the Heartbeat

Author(s): Medicine Maker/Stephanie Sutton, Katharina Kinast, Ulrike Becken, Robert Zweigerdt
Published in: 2017

"Editorial ""Controlled Stem Cell Production"""

Author(s): Ulrike Becken/ DASGIP
Published in: Eppendorf Customer Magazine BioNews, H2/2017 , 2017

Collaboration is key

Author(s): Medicine Maker/Stephanie Sutton, Katharina Kinast, Ulrike Becken, Robert Zweigerdt
Published in: The Medicine Maker’s Advancing Medicine supplement (print), 2017

A question of consistency: biopharma know-how meets bioreactor innovation in upstream bioprocessing




Expert View: Large-scale stem cell expansion and differentiation




Internationale Experten beim zweiten Stem Cell Community DAy

Author(s): LABO
Published in: LABO 7-8/2018, 2018

Towards functional analysis of cerebrovascular cell types derived from hiPSC

Author(s): Oleh Halaidych
Published in: 2019