Skip to main content

Systematic Genetic Code Reprogramming

Objective

We will build an orthogonal translation system for the encoded synthesis of unnatural polymers in cells. The steps towards this goal are each significant and substantial and, importantly, our solutions for each step are both scalable and modular with those for other steps in the process. In the first step we create a scalable and modular route to multiple new aminoacyl-tRNA synthetase/tRNA pairs that are orthogonal to each other and to endogenous synthetases and tRNAs. In a second step we engineer a cell for orthogonal translation to enable each orthogonal tRNA to quantitatively and efficiently decode a distinct quadruplet codon. In a third step we invent a modular and scalable route to program the active sites of mutually orthogonal synthetases to uniquely recognize distinct unnatural building blocks. The modular combination of the advances in each step enables the conversion of genetic code expansion with one or two unnatural amino acids into a scalable technology for genetic code reprogramming, facilitating the encoded synthesis of unnatural polymers using a synthetic genetic code with more encoded monomers than the natural genetic code.

Field of science

  • /natural sciences/chemical sciences/polymer science
  • /natural sciences/chemical sciences/organic chemistry/amines

Call for proposal

ERC-2014-ADG
See other projects for this call

Funding Scheme

ERC-ADG - Advanced Grant

Host institution

UNITED KINGDOM RESEARCH AND INNOVATION
Address
Polaris House North Star Avenue
SN2 1FL Swindon
United Kingdom
Activity type
Research Organisations
EU contribution
€ 2 475 344

Beneficiaries (1)

UNITED KINGDOM RESEARCH AND INNOVATION
United Kingdom
EU contribution
€ 2 475 344
Address
Polaris House North Star Avenue
SN2 1FL Swindon
Activity type
Research Organisations