Obiettivo We will build an orthogonal translation system for the encoded synthesis of unnatural polymers in cells. The steps towards this goal are each significant and substantial and, importantly, our solutions for each step are both scalable and modular with those for other steps in the process. In the first step we create a scalable and modular route to multiple new aminoacyl-tRNA synthetase/tRNA pairs that are orthogonal to each other and to endogenous synthetases and tRNAs. In a second step we engineer a cell for orthogonal translation to enable each orthogonal tRNA to quantitatively and efficiently decode a distinct quadruplet codon. In a third step we invent a modular and scalable route to program the active sites of mutually orthogonal synthetases to uniquely recognize distinct unnatural building blocks. The modular combination of the advances in each step enables the conversion of genetic code expansion with one or two unnatural amino acids into a scalable technology for genetic code reprogramming, facilitating the encoded synthesis of unnatural polymers using a synthetic genetic code with more encoded monomers than the natural genetic code. Campo scientifico scienze naturaliscienze biologichebiochimicabiomolecoleproteinescienze naturaliscienze chimichescienze dei polimeriscienze naturaliscienze chimichechimica organicaamminescienze naturaliscienze biologichegeneticagenomi Programma(i) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Argomento(i) ERC-ADG-2014 - ERC Advanced Grant Invito a presentare proposte ERC-2014-ADG Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-ADG - Advanced Grant Coordinatore UNITED KINGDOM RESEARCH AND INNOVATION Contribution nette de l'UE € 2 475 344,00 Indirizzo Polaris house north star avenue SN2 1FL Swindon Regno Unito Mostra sulla mappa Regione South West (England) Gloucestershire, Wiltshire and Bristol/Bath area Swindon Tipo di attività Research Organisations Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Altri finanziamenti € 0,00 Beneficiari (1) Classifica in ordine alfabetico Classifica per Contributo netto dell'UE Espandi tutto Riduci tutto UNITED KINGDOM RESEARCH AND INNOVATION Regno Unito Contribution nette de l'UE € 2 475 344,00 Indirizzo Polaris house north star avenue SN2 1FL Swindon Mostra sulla mappa Regione South West (England) Gloucestershire, Wiltshire and Bristol/Bath area Swindon Tipo di attività Research Organisations Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Altri finanziamenti € 0,00
