Understanding Cytokinetic Actomyosin Ring Assembly Through Genetic Code Expansion, Click Chemistry, DNA origami, and in vitro Reconstitution

Project Information

ACTOMYOSIN RING

Grant agreement ID: 671083

DOI 10.3030/671083

Project closed

EC signature date 30 October 2015

Start date 1 November 2015

End date 30 April 2021

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 863 705,00

EU contribution € 2 863 705,00

Coordinated by

UNIVERSITY OF WARWICK
United Kingdom

Publications

Peer reviewed articles (16)

Expanding the Zebrafish Genetic Code through Site-Specific Introduction of Azido-lysine, Bicyclononyne-lysine, and Diazirine-lysine

Author(s): Junetha Syed, Saravanan Palani, Scott Clarke, Zainab Asad, Andrew R Bottrill, Alexandra M E Jones, Karuna Sampath, Mohan K Balasubramanian
Published in: International Journal of Molecular Sciences, 2019, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.1101/631432

Inhibition of cell membrane ingression at the division site by cell wall in fission yeast

Author(s): Ting Gang Chew, Tzer Chyn Lim, Yumi Osaki, Junqi Huang, Tomoyuki Hatano, Anton Kamnev, Masako Osumi, Mohan K. Balasubramanian
Published in: Molecular Biology of the Cell, 2020, ISSN 1939-4586
Publisher: ASCB
DOI: 10.1091/mbc.e20-04-0245

Phosphoregulation of tropomyosin is crucial for actin cable turnover and division site placement

Author(s): Saravanan Palani, Darius V Köster, Tomoyuki Hatano, Anton Kamnev, Taishi Kanamaru, Holly R Brooker, Juan Ramon Hernandez-Fernaud, Alexandra M E Jones, Jonathan B A Millar, Daniel P Mulvihill, Mohan K Balasubramanian
Published in: Journal of Cell Biology, 2019, ISSN 0021-9525
Publisher: Rockefeller University Press
DOI: 10.1083/jcb.201809089

Phosphoregulation of tropomyosin-actin interaction revealed using a genetic code expansion strategy

Author(s): Saravanan Palani, Darius Koester, Mohan K Balasubramanian
Published in: Wellcome Open Research, 2020, ISSN 0000-0000
Publisher: F1000 Press
DOI: 10.12688/wellcomeopenres.16082.1

Calponin-Homology Domain mediated bending of membrane associated actin filaments

Author(s): Saravanan Palani, Sayantika Ghosh, Esther Ivorra-Molla, Scott Clarke, Andrejus Suchenko, Mohan K Balasubramanian, Darius Vasco Köster
Published in: eLife, 2021, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.61078

Actin turnover ensures uniform tension distribution during cytokinetic actomyosin ring contraction

Author(s): Tom Cheffings, Nigel Burroughs, Mohan Balasubramanian
Published in: Molecular Biology of the Cell, 2019, ISSN 1939-4586
Publisher: ASCB
DOI: 10.1091/mbc.e18-08-0511

Polar relaxation by dynein-mediated removal of cortical myosin II

Author(s): Bernardo Chapa-Y-Lazo, Motonari Hamanaka, Alexander Wray, Mohan K Balasubramanian, Masanori Mishima
Published in: Journal of Cell Biology, 2020, ISSN 0021-9525
Publisher: Rockefeller University Press
DOI: 10.1101/552372

Steric hindrance in the upper 50 kDa domain of the motor Myo2p leads to cytokinesis defects in fission yeast

Author(s): Saravanan Palani, Ramanujam Srinivasan, Paola Zambon, Anton Kamnev, Pananghat Gayathri, Mohan K. Balasubramanian
Published in: Journal of Cell Science, Issue 131/1, 2018, Page(s) jcs205625, ISSN 0021-9533
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/jcs.205625

Rapid production of pure recombinant actin isoforms in Pichia pastoris

Author(s): Tomoyuki Hatano, Salvatore Alioto, Emanuele Roscioli, Saravanan Palani, Scott T. Clarke, Anton Kamnev, Juan Ramon Hernandez-Fernaud, Lavanya Sivashanmugam, Bernardo Chapa-y-Lazo, Alexandra M. E. Jones, Robert C. Robinson, Karuna Sampath, Masanori Mishima, Andrew D. McAinsh, Bruce L. Goode, Mohan K. Balasubramanian
Published in: Journal of Cell Science, Issue 131/8, 2018, Page(s) jcs213827, ISSN 0021-9533
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/jcs.213827

Equatorial Assembly of the Cell-Division Actomyosin Ring in the Absence of Cytokinetic Spatial Cues

Author(s): Tzer Chyn Lim, Tomoyuki Hatano, Anton Kamnev, Mohan K. Balasubramanian, Ting Gang Chew
Published in: Current Biology, Issue 28/6, 2018, Page(s) 955-962.e3, ISSN 0960-9822
Publisher: Cell Press
DOI: 10.1016/j.cub.2018.01.088

Opposing kinesin complexes queue at plus tips to ensure microtubule catastrophe at cell ends

Author(s): John C Meadows, Liam J Messin, Anton Kamnev, Theresa C Lancaster, Mohan K Balasubramanian, Robert A Cross, Jonathan BA Millar
Published in: EMBO reports, 2018, Page(s) e46196, ISSN 1469-221X
Publisher: Nature Publishing Group
DOI: 10.15252/embr.201846196

Myo2p is the major motor involved in actomyosin ring contraction in fission yeast

Author(s): Paola Zambon, Saravanan Palani, Anton Kamnev, Mohan K. Balasubramanian
Published in: Current Biology, Issue 27/3, 2017, Page(s) R99-R100, ISSN 0960-9822
Publisher: Cell Press
DOI: 10.1016/j.cub.2016.12.024

Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction

Author(s): Ting Gang Chew, Junqi Huang, Saravanan Palani, Ruth Sommese, Anton Kamnev, Tomoyuki Hatano, Ying Gu, Snezhana Oliferenko, Sivaraj Sivaramakrishnan, Mohan K. Balasubramanian
Published in: The Journal of Cell Biology, 2017, Page(s) jcb.201701104, ISSN 0021-9525
Publisher: Rockefeller University Press
DOI: 10.1083/jcb.201701104

Motor Activity Dependent and Independent Functions of Myosin II Contribute to Actomyosin Ring Assembly and Contraction in Schizosaccharomyces pombe

Author(s): Saravanan Palani, Ting Gang Chew, Srinivasan Ramanujam, Anton Kamnev, Shrikant Harne, Bernardo Chapa-y-Lazo, Rebecca Hogg, Mayalagu Sevugan, Mithilesh Mishra, Pananghat Gayathri, Mohan K. Balasubramanian
Published in: Current Biology, Issue 27/5, 2017, Page(s) 751-757, ISSN 0960-9822
Publisher: Cell Press
DOI: 10.1016/j.cub.2017.01.028

Genetic suppression of defective profilin by attenuated Myosin II reveals a potential role for Myosin II in actin dynamics in vivo in fission yeast

Author(s): Paola Zambon, Saravanan Palani, Shekar Jadhav, Pananghat Gayathri, Mohan Balasubramanian
Published in: Molecular Biology of the Cell, 2020, ISSN 1939-4586
Publisher: ASCB
DOI: 10.1101/2020.04.03.023705

Pick-ya actin - a method to purify actin isoforms with bespoke key post-translational modifications

Author(s): Tomoyuki Hatano, Lavanya Sivashanmugam, Andrejus Suchenko, Hamdi Hussain, Mohan K Balasubramanian
Published in: Journal of Cell Science, 2020, ISSN 0021-9533
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/jcs.241406