Elucidation of autophagy using novel chemical probes

Project Information

ChemBioAP

Grant agreement ID: 678899

DOI

10.3030/678899

Project closed

EC signature date 14 March 2016

Start date 1 September 2016

End date 31 August 2021

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 500 000,00

EU contribution

€ 1 500 000,00

Coordinated by

UMEA UNIVERSITET
Sweden

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (25)

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1

Author(s): Daniel J. Klionsky, Amal Kamal Abdel-Aziz, Sara Abdelfatah, Mahmoud Abdellatif, Asghar Abdoli, Steffen Abel, Hagai Abeliovich, Marie H. Abildgaard, Yakubu Princely Abudu, Abraham Acevedo-Arozena, Iannis E. Adamopoulos, Khosrow Adeli, Timon E. Adolph, Annagrazia Adornetto, Elma Aflaki, Galila Agam, Anupam Agarwal, Bharat B. Aggarwal, Maria Agnello, Patrizia Agostinis, Javed N. Agrewala, Alexander A
Published in: Autophagy, Issue 17/1, 2021, Page(s) 1-382, ISSN 1554-8627
Publisher: Landes Bioscience
DOI: 10.1080/15548627.2020.1797280

Phenotyping Reveals Targets of a Pseudo‐Natural‐Product Autophagy Inhibitor

Author(s): Daniel J. Foley, Sarah Zinken, Dale Corkery, Luca Laraia, Axel Pahl, Yao‐Wen Wu, Herbert Waldmann
Published in: Angewandte Chemie International Edition, Issue 59/30, 2020, Page(s) 12470-12476, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202000364

Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network

Author(s): Dominic Kamps, Johannes Koch, Victor O. Juma, Eduard Campillo-Funollet, Melanie Graessl, Soumya Banerjee, Tomáš Mazel, Xi Chen, Yao-Wen Wu, Stephanie Portet, Anotida Madzvamuse, Perihan Nalbant, Leif Dehmelt
Published in: Cell Reports, Issue 33/9, 2020, Page(s) 108467, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2020.108467

Thermal proteome profiling identifies the membrane-bound purinergic receptor P2X4 as a target of the autophagy inhibitor indophagolin

Author(s): Marjorie A. Carnero Corrales, Sarah Zinken, Georgios Konstantinidis, Muhammad Rafehi, Aliaa Abdelrahman, Yao-Wen Wu, Petra Janning, Christa E. Müller, Luca Laraia, Herbert Waldmann
Published in: Cell Chemical Biology, 2021, ISSN 2451-9456
Publisher: Cell Press
DOI: 10.1016/j.chembiol.2021.02.017

Vibrio cholerae cytotoxin MakA induces noncanonical autophagy resulting in the spatial inhibition of canonical autophagy

Author(s): Dale P. Corkery, Aftab Nadeem, Kyaw Min Aung, Ahmed Hassan, Tao Liu, Ramón Cervantes-Rivera, Alf Håkon Lystad, Hui Wang, Karina Persson, Andrea Puhar, Anne Simonsen, Bernt Eric Uhlin, Sun Nyunt Wai, Yao-Wen Wu
Published in: Journal of Cell Science, 2021, ISSN 0021-9533
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/jcs.252015

Elucidation of the anti-autophagy mechanism of the Legionella effector RavZ using semisynthetic LC3 proteins

Author(s): Aimin Yang, Supansa Pantoom, Yao-Wen Wu
Published in: eLife, Issue 6, 2017, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/eLife.23905

Lift and cut: Anti-host autophagy mechanism of Legionella pneumophila

Author(s): Supansa Pantoom, Aimin Yang, Yao-Wen Wu
Published in: Autophagy, Issue 13/8, 2017, Page(s) 1467-1469, ISSN 1554-8627
Publisher: Landes Bioscience
DOI: 10.1080/15548627.2017.1327943

Phenotypic Identification of a Novel Autophagy Inhibitor Chemotype Targeting Lipid Kinase VPS34

Author(s): Lucas Robke, Luca Laraia, Marjorie A. Carnero Corrales, Georgios Konstantinidis, Makoto Muroi, André Richters, Michael Winzker, Tobias Engbring, Stefano Tomassi, Nobumoto Watanabe, Hiroyuki Osada, Daniel Rauh, Herbert Waldmann, Yao-Wen Wu, Julian Engel
Published in: Angewandte Chemie International Edition, Issue 56/28, 2017, Page(s) 8153-8157, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201703738

Semisynthesis of autophagy protein LC3 conjugates

Author(s): Aimin Yang, Inken Hacheney, Yao-Wen Wu
Published in: Bioorganic & Medicinal Chemistry, Issue 25/18, 2017, Page(s) 4971-4976, ISSN 0968-0896
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.bmc.2017.05.051

“Molecular Activity Painting”: Switch-like, Light-Controlled Perturbations inside Living Cells

Author(s): Xi Chen, Muthukumaran Venkatachalapathy, Dominic Kamps, Simone Weigel, Ravi Kumar, Michael Orlich, Ruben Garrecht, Michael Hirtz, Christof M. Niemeyer, Yao-Wen Wu, Leif Dehmelt
Published in: Angewandte Chemie International Edition, Issue 56/21, 2017, Page(s) 5916-5920, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201611432

Discovery of Novel Cinchona-Alkaloid-Inspired Oxazatwistane Autophagy Inhibitors

Author(s): Luca Laraia, Kosuke Ohsawa, Georgios Konstantinidis, Lucas Robke, Yao-Wen Wu, Kamal Kumar, Herbert Waldmann
Published in: Angewandte Chemie International Edition, Issue 56/8, 2017, Page(s) 2145-2150, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201611670

Tunable and Photoswitchable Chemically Induced Dimerization for Chemo-optogenetic Control of Protein and Organelle Positioning

Author(s): Xi Chen, Yao-Wen Wu
Published in: Angewandte Chemie International Edition, Issue 57/23, 2018, Page(s) 6796-6799, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201800140

Discovery of the novel autophagy inhibitor aumitin that targets mitochondrial complex I

Author(s): Lucas Robke, Yushi Futamura, Georgios Konstantinidis, Julian Wilke, Harumi Aono, Zhwan Mahmoud, Nobumoto Watanabe, Yao-Wen Wu, Hiroyuki Osada, Luca Laraia, Herbert Waldmann
Published in: Chemical Science, Issue 9/11, 2018, Page(s) 3014-3022, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/c7sc05040b

Spatial Cycling of Rab GTPase, Driven by the GTPase Cycle, Controls Rab’s Subcellular Distribution

Author(s): Stephanie Voss, Fu Li, Andreas Rätz, Matthias Röger, Yao-Wen Wu
Published in: Biochemistry, Issue 58/4, 2019, Page(s) 276-285, ISSN 0006-2960
Publisher: American Chemical Society
DOI: 10.1021/acs.biochem.8b00932

Multidirectional Activity Control of Cellular Processes by a Versatile Chemo-optogenetic Approach

Author(s): Xi Chen, Muthukumaran Venkatachalapathy, Leif Dehmelt, Yao-Wen Wu
Published in: Angewandte Chemie International Edition, Issue 57/37, 2018, Page(s) 11993-11997, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201806976

Modulation of autophagy by the novel mitochondrial complex I inhibitor Authipyrin

Author(s): Nadine Kaiser, Dale Corkery, Yaowen Wu, Luca Laraia, Herbert Waldmann
Published in: Bioorganic & Medicinal Chemistry, 2019, ISSN 0968-0896
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.bmc.2019.02.028

Bacterial interaction with host autophagy

Author(s): Yao-Wen Wu, Fu Li
Published in: Virulence, Issue 10/1, 2019, Page(s) 352-362, ISSN 2150-5594
Publisher: Landes Bioscience
DOI: 10.1080/21505594.2019.1602020

Light‐Induced Dimerization Approaches to Control Cellular Processes

Author(s): Laura Klewer, Yao‐Wen Wu
Published in: Chemistry – A European Journal, Issue 25/54, 2019, Page(s) 12452-12463, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201900562

Toward the role of cholesterol and cholesterol transfer protein in autophagosome biogenesis

Author(s): Yao-Wen Wu, Herbert Waldmann
Published in: Autophagy, Issue 15/12, 2019, Page(s) 2167-2168, ISSN 1554-8627
Publisher: Landes Bioscience
DOI: 10.1080/15548627.2019.1666595

Image-Based Morphological Profiling Identifies a Lysosomotropic, Iron-Sequestering Autophagy Inhibitor

Author(s): Luca Laraia, Guillaume Garivet, Daniel J. Foley, Nadine Kaiser, Sebastian Müller, Sarah Zinken, Thomas Pinkert, Julian Wilke, Dale Corkery, Axel Pahl, Sonja Sievers, Petra Janning, Christoph Arenz, Yaowen Wu, Raphaël Rodriguez, Herbert Waldmann
Published in: Angewandte Chemie International Edition, 2019, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201913712

Electroporated recombinant proteins as tools for in vivo functional complementation, imaging and chemical biology

Author(s): Amal Alex, Valentina Piano, Soumitra Polley, Marchel Stuiver, Stephanie Voss, Giuseppe Ciossani, Katharina Overlack, Beate Voss, Sabine Wohlgemuth, Arsen Petrovic, Yaowen Wu, Philipp Selenko, Andrea Musacchio, Stefano Maffini
Published in: eLife, Issue 8, 2019, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.48287

The cholesterol transfer protein GRAMD1A regulates autophagosome biogenesis

Author(s): Luca Laraia, Alexandra Friese, Dale P. Corkery, Georgios Konstantinidis, Nelli Erwin, Walter Hofer, Hacer Karatas, Laura Klewer, Andreas Brockmeyer, Malte Metz, Beate Schölermann, Mridula Dwivedi, Lei Li, Pablo Rios-Munoz, Maja Köhn, Roland Winter, Ingrid R. Vetter, Slava Ziegler, Petra Janning, Yao-Wen Wu, Herbert Waldmann
Published in: Nature Chemical Biology, Issue 15/7, 2019, Page(s) 710-720, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-019-0307-5

Inhibition of K-Ras4B-plasma membrane association with a membrane microdomain-targeting peptide

Author(s): Fang-Yi Li, Zhen-Feng Zhang, Stephanie Voss, Yao-Wen Wu, Yu-Fen Zhao, Yan-Mei Li, Yong-Xiang Chen
Published in: Chemical Science, Issue 11/3, 2020, Page(s) 826-832, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/c9sc04726c

Distinct Mechanisms for Processing Autophagy Protein LC3‐PE by RavZ and ATG4B

Author(s): Aimin Yang, Supansa Pantoom, Yao‐Wen Wu
Published in: ChemBioChem, Issue 21/23, 2020, Page(s) 3377-3382, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000359

RAB33B recruits the ATG16L1 complex to the phagophore via a noncanonical RAB binding protein

Author(s): Supansa Pantoom, Georgios Konstantinidis, Stephanie Voss, Hongmei Han, Oliver Hofnagel, Zhiyu Li, Yao-Wen Wu
Published in: Autophagy, 2020, Page(s) 1-15, ISSN 1554-8627
Publisher: Landes Bioscience
DOI: 10.1080/15548627.2020.1822629

Book chapters (5)

Imaging of Spatial Cycling of Rab GTPase in the Cell

Author(s): Fu Li, Yao-Wen Wu
Published in: Rab GTPases - Methods and Protocols, Issue 2293, 2021, Page(s) 105-115, ISBN 978-1-0716-1345-0
Publisher: Springer US
DOI: 10.1007/978-1-0716-1346-7_8

Identification of Novel Autophagy Inhibitors via Cell-Based High-Content Screening

Author(s): Georgios Konstantinidis, Sonja Sievers, Yao-Wen Wu
Published in: Methods in Molecular Biology, 2018, ISBN 978-1-4939-8747-4
Publisher: Humana Press
DOI: 10.1007/7651_2018_125

Generation of Intramolecular FRET Probes via Noncanonical Amino Acid Mutagenesis

Author(s): Simone Brand, Yao-Wen Wu
Published in: Methods in Molecular Biology, 2018, Page(s) 327-335, ISBN 978-1-4939-7573-0
Publisher: Springer New York
DOI: 10.1007/978-1-4939-7574-7_21

Affinity Conjugation for Rapid and Covalent Labeling of Proteins in Live Cells

Author(s): Xi Chen, Fu Li, Yao-Wen Wu
Published in: Proximity Labeling - Methods and Protocols, Issue 2008, 2019, Page(s) 191-202, ISBN 978-1-4939-9536-3
Publisher: Springer New York
DOI: 10.1007/978-1-4939-9537-0_15

Spatiotemporal Imaging of Small GTPase Activity Using Conformational Sensors for GTPase Activity (COSGA)

Author(s): Yao-Wen Wu
Published in: Ras Activity and Signaling - Methods and Protocols, Issue 2262, 2021, Page(s) 259-267, ISBN 978-1-0716-1189-0
Publisher: Springer US
DOI: 10.1007/978-1-0716-1190-6_15

Intellectual Property Rights

Patent (1)

PHOTO-SWITCHABLE CHEMICAL INDUCERS OF DIMERIZATION FOR CONTROL OF PROTEIN FUNCTION IN CELLS BY LIGHT

Application/Publication number: 18 735591
Date: 2018-07-06
Applicant(s): MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV