Periodic Reporting for period 2 - T6S (Multi-scale model of bacterial cell-cell interactions)
Reporting period: 2018-07-01 to 2019-12-31
Towards Aim 2, we established the mechanism of T6SS bundle formation in Amoebophilus asiaticus (Böck et al., Science). Ongoing efforts address this issue in Pseudomonas aeruginosa. Furthermore, we showed a mechanism for T6SS length regulation in EAEC (Szwedziak and Pilhofer, Nature Communications).
Towards Aim 3, we established a workflow for cryo-focused ion beam milling in order to thin samples for cryo-electron tomography (Medeiros et al., Ultramicroscopy). Subsequently, we showed the suitability of the method to answer biological questions (Cai et al., Molecular Biology of the Cell). We also used the method in order to study the function of the Amoebophilus T6SS (Böck et al., Science). Furthermore, we established cryo-light microscopy for the identification of rare targets (Szwedziak and Pilhofer, Nature Communications).