Objective RNA plays a central role in the regulation of gene expression. The basal levels of RNA in a cell depend on the ratio between RNA synthesis and RNA degradation. RNA degradation is an active and critical process that dictates RNA levels and in part controls the relative levels of gene expression. However, despite many years of research, important and perplexing questions remain about RNA stability. Even when comparable RNA degradation processes are involved individual RNAs can have distinct decay rates and the mechanisms that control such distinctive rates are unknown. RNA structure is likely to be intrinsic to our understanding of the RNA features that govern stability and until very recently our ability to measure the true in vivo RNA structure has been incredibly limited. The aim of this proposal is to understand the role of RNA structure in the regulation of RNA degradation and determine the RNA structural features that regulate the degradation pathways. We propose an ambitious programme that will pursue the following objectives: 1. Globally investigate RNA structural features and compare these to RNA degradation in vivo. 2. Decipher the mechanism of no go decay (NGD) via identifying the role of the G-quadruplex.3. Determine the role of RNA structure in the miRNA pathway for both miRNA precursor processing and miRNA-directed processing.This proposed study will reveal the fundamental function of RNA structures in RNA degradation. Two novel in vivo RNA structural profiling platforms will be established. The combination of RNA structural profiling, with existing ribosome profiling and the RNA degradome will generate a global view of RNA structural features and their relationship with RNA degradation. This proposed study will fill a significant gap in our understanding of the mechanisms of RNA degradation in plants and this will likely impact RNA studies in all eukaryotes. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticsnucleotidesnatural sciencesbiological sciencesgeneticsRNAnatural sciencesbiological sciencesmolecular biology Programme(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Topic(s) ERC-StG-2015 - ERC Starting Grant Call for proposal ERC-2015-STG See other projects for this call Funding Scheme ERC-STG - Starting Grant Host institution JOHN INNES CENTRE Net EU contribution € 1 499 986,00 Address NORWICH RESEARCH PARK COLNEY NR4 7UH Norwich United Kingdom See on map Region East of England East Anglia Breckland and South Norfolk Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 1 499 986,00 Beneficiaries (1) Sort alphabetically Sort by Net EU contribution Expand all Collapse all JOHN INNES CENTRE United Kingdom Net EU contribution € 1 499 986,00 Address NORWICH RESEARCH PARK COLNEY NR4 7UH Norwich See on map Region East of England East Anglia Breckland and South Norfolk Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 1 499 986,00
