Why does Clostridium botulinum kill? – In search for botulinum neurotoxin regulators

Project Information

whyBOTher

Grant agreement ID: 683099

DOI

10.3030/683099

Project closed

EC signature date 28 November 2016

Start date 1 January 2017

End date 31 December 2023

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 2 000 000,00

EU contribution

€ 2 000 000,00

Coordinated by

HELSINGIN YLIOPISTO
Finland

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Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (11)

Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing

Author(s): Cedric Woudstra, Tommi Mäklin, Yagmur Derman, Luca Bano, Hanna Skarin, Christelle Mazuet, Antti Honkela, Miia Lindström
Published in: Microbiology Resource Announcements, Issue 10/22, 2021, ISSN 2576-098X
Publisher: ASM
DOI: 10.1128/mra.01364-20

CRISPR-Cas9-Based Toolkit for Clostridium botulinum Group II Spore and Sporulation Research

Author(s): Anna Mertaoja, Maria B Nowakowska, Gerald Mascher, Viivi Heljanko, Daphne Groothuis, Nigel P Minton, Miia Lindström
Published in: Frontiers in Microbiology, 2021, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2021.617269

Sporulation Strategies and Potential Role of the Exosporium in Survival and Persistence of Clostridium botulinum

Author(s): Inês M. Portinha; François P. Douillard; Hannu Korkeala; Miia Lindström
Published in: International Journal of Molecular Sciences, Vol 23, Iss 754, p 754 (2022), Issue 1, 2022, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23020754

Crystal structures of OrfX1, OrfX2 and the OrfX1-OrfX3 complex from the orfX gene cluster of botulinum neurotoxin E1

Author(s): Linfeng Gao, Kwok-Ho Lam, Shun Liu , Adina Przykopanski, Johanna Lübke, Ruifeng Qi, Maren Krüger, Maria B Nowakowska, Katja Selby, François P Douillard, Martin B Dorner, Kay Perry, Miia Lindström, Brigitte G Dorner, Andreas Rummel, Rongsheng Jin
Published in: FEBS Letters, 2023, ISSN 0014-5793
Publisher: Elsevier BV
DOI: 10.1002/1873-3468.14576

A Novel Prophage-like Insertion Element within yabG Triggers Early Entry into Sporulation in Clostridium botulinum

Author(s): François P Douillard, Inês Martins Portinha, Yağmur Derman, Cédric Woudstra, Tommi Mäklin, Martin B Dorner, Hannu Korkeala, Adriano O Henriques, Miia Lindström
Published in: Viruses, Issue 15(12):2431, 2023, ISSN 1999-4915
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v15122431

Construction and validation of safe Clostridium botulinum Group II surrogate strain producing inactive botulinum neurotoxin type E toxoid

Author(s): Maria B Nowakowska, Katja Selby, Adina Przykopanski, Maren Krüger, Nadja Krez, Brigitte G Dorner, Martin B Dorner, Rongsheng Jin, Nigel P Minton, Andreas Rummel, Miia Lindström
Published in: Scientific Reports, 2022, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-022-05008-1

Cellular and population strategies underpinning neurotoxin production and sporulation in Clostridium botulinum type E cultures

Author(s): Anna Mertaoja, Gerald Mascher, Maria B Nowakowska, Hannu Korkeala, Adriano O Henriques, Miia Lindstrom
Published in: mBio, Issue 14(6), 2023, ISSN 2150-7511
Publisher: ASM
DOI: 10.1128/mbio.01866-23

Genomic and Phenotypic Characterization of Clostridium botulinum Isolates from an Infant Botulism Case Suggests Adaptation Signatures to the Gut

Author(s): François P Douillard, Yağmur Derman, Cédric Woudstra, Katja Selby, Tommi Mäklin, Martin B Dorner, Harri Saxén, Brigitte G Dorner, Hannu Korkeala, Miia Lindström
Published in: mBio, 2022, ISSN 2150-7511
Publisher: ASM
DOI: 10.1128/mbio.02384-21

Pan-Genomic Analysis of Clostridium botulinum Group II (Non-Proteolytic C. botulinum) Associated with Foodborne Botulism and Isolated from the Environment

Author(s): Jason Brunt, Arnoud H. M. van Vliet, Sandra C. Stringer, Andrew T. Carter, Miia Lindström, and Michael W. Peck
Published in: Toxins, 2020, ISSN 2072-6651
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins12050306

Neurotoxin synthesis is positively regulated by the sporulation transcription factor Spo0A in Clostridium botulinum type E

Author(s): Gerald Mascher, Anna Mertaoja, Hannu Korkeala, Miia Lindström
Published in: Environmental Microbiology, Issue 19/10, 2017, Page(s) 4287-4300, ISSN 1462-2912
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.13892

Looking for the X Factor in Bacterial Pathogenesis: Association of orfX-p47 Gene Clusters with Toxin Genes in Clostridial and Non-Clostridial Bacterial Species

Author(s): Maria B. Nowakowska, François P. Douillard, Miia Lindström
Published in: Toxins, Issue 12/1, 2020, Page(s) 19, ISSN 2072-6651
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins12010019

Conference proceedings (2)

A longitudinal genomic and functional analysis of Clostridium botulinum isolated from an infant botulism case

Author(s): François P. Douillard, Yağmur Derman, Cédric Woudstra, Katja Selby, Tommi Mäklin, Antti Honkela, Hannu Korkeala, Miia Lindström
Published in: Toxicon, Issue 190, 2021, Page(s) S17, ISSN 0041-0101
Publisher: Pergamon Press
DOI: 10.1016/j.toxicon.2020.11.374

Utilization of a phenotypic microarray for screening of Clostridium botulinum growth-enhancing substrates

Author(s): Katja Selby, François P. Douillard, Miia Lindström
Published in: Toxicon, Issue 190, 2021, Page(s) S68, ISSN 0041-0101
Publisher: Pergamon Press
DOI: 10.1016/j.toxicon.2020.11.487