Objective
Metagenomes comprise enormous reservoirs coding for proteins with useful activities. Unfortunately, harvesting this reservoir is difficult, because useful candidates are rare and hidden in an overwhelming majority of irrelevant genes. Screening campaigns of metagenomic libraries thus require massive capital-expenditure for robotic systems and much manpower, making them expensive, slow and available to very few users. To enable valorisation of the potential of the metagenome, this project assembles an interdisciplinary and intersectoral consortium that will integrate a range of technologies into a platform designed to beat the odds of identifying library hits faster, more efficiently and by a wider user base. Exploration and exploitation of the metagenome will be made faster and more successful by (i) ultrahigh-throughput screening in picoliter droplets that dramatically lowers the cost per assay to well below 0.01 cents and allows throughput of 10e7 assays per hour; (ii) workflows that streamline and increase the yield of library construction and functional expression and (iii) workflows for efficient bioinformatic analysis of hits based on user-friendly software solutions for metagenome analysis. Emphasis is put on technologies that are straightforwardly implemented in non-specialist labs, maximising the impact of METAFLUIDICS. This platform will be used to identify enzymes for biosynthesis of therapeutic small molecules, for green bioenergy conversion, bioremediation, food chemistry and other industrial applications
Fields of science
- natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidics
- natural sciencescomputer and information sciencessoftware
- engineering and technologyenvironmental biotechnologybioremediation
- natural sciencesbiological sciencesmicrobiology
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
Programme(s)
Funding Scheme
RIA - Research and Innovation action
Coordinator
28049 Madrid
Spain
See on map
Participants (15)
8000 Aarhus
See on map
18100 Armilla Granada
See on map
The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.
CB4 3QU Cambridge
See on map
The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.
NE49 9HN Haltwhistle
See on map
The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.
CB2 1TN Cambridge
See on map
2880 Bagsvaerd
See on map
31077 Toulouse Cedex 4
See on map
Legal entity other than a subcontractor which is affiliated or legally linked to a participant. The entity carries out work under the conditions laid down in the Grant Agreement, supplies goods or provides services for the action, but did not sign the Grant Agreement. A third party abides by the rules applicable to its related participant under the Grant Agreement with regard to eligibility of costs and control of expenditure.
75007 Paris Cedex 07
See on map
3004 517 Coimbra
See on map
03690 Alicante
See on map
28006 Madrid
See on map
Legal entity other than a subcontractor which is affiliated or legally linked to a participant. The entity carries out work under the conditions laid down in the Grant Agreement, supplies goods or provides services for the action, but did not sign the Grant Agreement. A third party abides by the rules applicable to its related participant under the Grant Agreement with regard to eligibility of costs and control of expenditure.
28850 Torrejon De Ardoz Madrid
See on map
7491 Trondheim
See on map
Participation ended
7034 Trondheim
7034 Trondheim
See on map