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Advanced toolbox for rapid and cost-effective functional metagenomic screening - microbiology meets microfluidics.

Objective

Metagenomes comprise enormous reservoirs coding for proteins with useful activities. Unfortunately, harvesting this reservoir is difficult, because useful candidates are rare and hidden in an overwhelming majority of irrelevant genes. Screening campaigns of metagenomic libraries thus require massive capital-expenditure for robotic systems and much manpower, making them expensive, slow and available to very few users. To enable valorisation of the potential of the metagenome, this project assembles an interdisciplinary and intersectoral consortium that will integrate a range of technologies into a platform designed to beat the odds of identifying library hits faster, more efficiently and by a wider user base. Exploration and exploitation of the metagenome will be made faster and more successful by (i) ultrahigh-throughput screening in picoliter droplets that dramatically lowers the cost per assay to well below 0.01 cents and allows throughput of 10e7 assays per hour; (ii) workflows that streamline and increase the yield of library construction and functional expression and (iii) workflows for efficient bioinformatic analysis of hits based on user-friendly software solutions for metagenome analysis. Emphasis is put on technologies that are straightforwardly implemented in non-specialist labs, maximising the impact of METAFLUIDICS. This platform will be used to identify enzymes for biosynthesis of therapeutic small molecules, for green bioenergy conversion, bioremediation, food chemistry and other industrial applications

Call for proposal

H2020-LEIT-BIO-2015-1
See other projects for this call

Coordinator

UNIVERSIDAD AUTONOMA DE MADRID
Address
Calle Einstein 3 Ciudad Univ Cantoblanco Rectorado
28049 Madrid
Spain
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 1 233 729

Participants (13)

QIAGEN AARHUS AS
Denmark
EU contribution
€ 463 750
Address
Silkeborgvej 2
8000 Aarhus
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
BIOILIBERIS RESEARCH AND DEVELOPMENT SL
Spain
EU contribution
€ 460 000
Address
Av De La Innovacion 1 Parque Tecnologico De Ciencias Salud
18100 Armilla Granada
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
DROP-TECH LTD
United Kingdom
EU contribution
€ 207 820
Address
Canterbury Street, Canterbury Court 91
CB4 3QU Cambridge
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
PROZOMIX LIMITED
United Kingdom
EU contribution
€ 546 806,25
Address
Station Court 3
NE49 9HN Haltwhistle
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
United Kingdom
EU contribution
€ 1 120 190
Address
Trinity Lane The Old Schools
CB2 1TN Cambridge
Activity type
Higher or Secondary Education Establishments
NOVOZYMES A/S
Denmark
EU contribution
€ 821 729
Address
Krogshoejvej 36
2880 Bagsvaerd
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE
France
EU contribution
€ 722 255
Address
Avenue De Rangueil 135
31077 Toulouse
Activity type
Higher or Secondary Education Establishments
CENTRO DE NEUROCIENCIAS E BIOLOGIACELULAR ASSOCIACAO
Portugal
EU contribution
€ 407 590
Address
Universidade De Coimbra Largo Marques De Pombal
3004 517 Coimbra
Activity type
Research Organisations
UNIVERSIDAD DE ALICANTE
Spain
EU contribution
€ 595 600
Address
Campus De San Vicente Raspeig
03690 Alicante
Activity type
Higher or Secondary Education Establishments
AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain
EU contribution
€ 636 282,50
Address
Calle Serrano 117
28006 Madrid
Activity type
Research Organisations
NORGES TEKNISK-NATURVITENSKAPELIGE UNIVERSITET NTNU
Norway
EU contribution
€ 840 357,50
Address
Hogskoleringen 1
7491 Trondheim
Activity type
Higher or Secondary Education Establishments
STIFTELSEN SINTEF

Participation ended

Norway
EU contribution
€ 291 006,41
Address
Strindveien 4
7034 Trondheim
Activity type
Research Organisations
SINTEF AS
Norway
EU contribution
€ 461 247,34
Address
Strindvegen 4
7034 Trondheim
Activity type
Research Organisations