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Innovative technology for the detection of enzyme activity in milk

Deliverables

Peer reviewed articles

Publication of scientific articles in per reviewed journals

Report on secondments

Reports on secondments finished. Will be prepared continuously during whole project duration.

Protocol of enzyme assay in a volume

Protocol of enzyme assay in a volume applicable for Industrial project partners and end-users

Data Management Plan

"Participation in the ""Open Research Data Pilot"" in Horizon2020 which aims to improve and maximise access to and re-use of research data generated by actions, a data management plan will be written. The plan will describe which of the research data that will be generated during the project will be made open. "

First progress report

First project progress report.

Protocol of enzyme assay at surfaces

Protocol of enzyme assay at surfaces applicable for Industrial project partners and end users

Second progress report

Third project progress report.

Mid-term meeting

There will be only 1 review meeting per project with timing between month 14 and 18, definitive date is to be confirmed by the COO and preferably linked to a consortium event

Workshops and summer schools

Organisation of summer schools and workshops

Miniature potentiostat, software and sensor

Construction of miniature potentiostat and software development for applicaation by Industrial project partners and end users

Website

Establishment of the web site of the project.

Searching for OpenAIRE data...

Publications

New technologies for nanoparticles detection in foods

Author(s): G. Castillo, Z. Garaiova, T. Hianik
Published in: Advanced Diagnostics for Food, Issue 2017, 2017, Page(s) 305-341

Affinity biosensors for detection mycotoxins in food




Nanomaterials in the Assembly of Electrochemical DNA Sensors

Author(s): G. Evtugyn, A. Porfireva, H. Budnikov, T. Hianik
Published in: Nanoanalytics. Nanoobjects and Nanotechnologies in Analytical Chemistry, Issue 2018, 2018, Page(s) 253-300
DOI: 10.1515/9783110542011-008

Ultrasonic Monitoring of Biocatalysis in Solutions and Complex Dispersions

Author(s): V. Buckin , M. Altas
Published in: Catalysts, Issue 7/11, 2017, Page(s) 336, ISSN 2073-4344
DOI: 10.3390/catal7110336

High-resolution ultrasonic spectroscopy

Author(s): Vitaly Buckin
Published in: Journal of Sensors and Sensor Systems, Issue 7/1, 2018, Page(s) 207-217, ISSN 2194-878X
DOI: 10.5194/jsss-7-207-2018

Surface properties of polyene glycol phospholipid monolayers

Author(s): Tibor Hianik, Christer L. Øpstad, Jana Šandorová, Zuzana Garaiová, Vassilia Partali, Hans-Richard Sliwka
Published in: Chemistry and Physics of Lipids, Issue 202, 2017, Page(s) 13-20, ISSN 0009-3084
DOI: 10.1016/j.chemphyslip.2016.11.007

Machine learning enabled acoustic detection of sub-nanomolar concentration of trypsin and plasmin in solution

Author(s): Marek Tatarko, Eric S. Muckley, Veronika Subjakova, Monojoy Goswami, Bobby G. Sumpter, Tibor Hianik, Ilia N. Ivanov
Published in: Sensors and Actuators B: Chemical, Issue 272, 2018, Page(s) 282-288, ISSN 0925-4005
DOI: 10.1016/j.snb.2018.05.100

Casein probe–based fast plasmin determination in the picomolar range by an ultra-high frequency acoustic wave biosensor

Author(s): Loránd Románszki, Marek Tatarko, Mengchi Jiao, Zsófia Keresztes, Tibor Hianik, Michael Thompson
Published in: Sensors and Actuators B: Chemical, Issue 275, 2018, Page(s) 206-214, ISSN 0925-4005
DOI: 10.1016/j.snb.2018.08.025

Label-Free and Redox Markers-Based Electrochemical Aptasensors for Aflatoxin M1 Detection

Author(s): Stefanos Karapetis, Dimitrios Nikolelis, Tibor Hianik
Published in: Sensors, Issue 18/12, 2018, Page(s) 4218, ISSN 1424-8220
DOI: 10.3390/s18124218

Electrochemical Immuno- and Aptasensors for Mycotoxin Determination

Author(s): Gennady Evtugyn, Tibor Hianik
Published in: Chemosensors, Issue 7/1, 2019, Page(s) 10, ISSN 2227-9040
DOI: 10.3390/chemosensors7010010

Combination of Ruthenium Dendrimers and Acoustically Propelled Gold Nanowires as a Platform for Active Intracellular Drug Delivery Towards Breast Cancer Therapy

Author(s): Zuzana Garaiová, Gulcin Bolat, Berta Esteban-Fernández de Ávila, Hua Gong, Natalia Sanz del Olmo, Paula Ortega, Francisco Javier de la Mata, Sylwia Michlewska, Joseph Wang, Tibor Hianik
Published in: Clinical Oncology and Research, 2019, Page(s) 1-5, ISSN 2613-4942
DOI: 10.31487/j.cor.2019.04.08

DNA-Polylactide Modified Biosensor for Electrochemical Determination of the DNA-Drugs and Aptamer-Aflatoxin M1 Interactions

Author(s): Veronika Stepanova, Vladimir Smolko, Vladimir Gorbatchuk, Ivan Stoikov, Gennady Evtugyn, Tibor Hianik
Published in: Sensors, Issue 19/22, 2019, Page(s) 4962, ISSN 1424-8220
DOI: 10.3390/s19224962

State of Oxygen Molecules in Aqueous Supersaturated Solutions

Author(s): Yuelong Li, Vitaly Buckin
Published in: The Journal of Physical Chemistry B, Issue 123/18, 2019, Page(s) 4025-4043, ISSN 1520-6106
DOI: 10.1021/acs.jpcb.9b01057

Plasmin protease and importance of this detection for milk industry

Author(s): Z. Keresztes
Published in: "International workshop ""Novel approaches for detection proteases in milk""", Issue February 2017, 2017, Page(s) 8

Development and testing of different acoustic biosensors for proteolytic activity detection on immobilised casein layer

Author(s): M. Tatarko, M. Thompson, I.N. Ivanov, T. Hianik
Published in: "International workshop ""Novel approaches for detection proteases in milk""", Issue February 2017, 2017, Page(s) 10

Casein assembly for gravimetric and optical detection of protease activity

Author(s): M. Tatarko, V. Šubjaková, E.S. Muckley, T. Hianik, I.N. Ivanov
Published in: "International workshop ""Novel approaches for detection proteases in milk""", Issue February 2017, 2017, Page(s) 11

Detection of chymotrypsin activity using QCM method

Author(s): I. Piovarči, A. Poturnayová, J. Nagy, R. Sarok, A. Hucker, K. Szabó, R. Kocsis, T. Hianik
Published in: "International workshop ""Novel approaches for detection proteases in milk""", Issue February 2017, 2017, Page(s) 13

High sensitive electrochemical aptasensor for detection aflatoxin M1 in milk

Author(s): S. Karapetis, M. Eliášová Sohová, L. Bábelová, A. Poturnayova, A. Hucker, K. Szabó, R. Kocsis, D.P. Nikolelis, T. Hianik
Published in: "International workshop ""Novel approaches for detection proteases in milk""", Issue February 2017, 2017, Page(s) 14

Enzymatic degradation of lipid - embedded β - casein in two dimensions: Langmuir monolayer studies

Author(s): L. Románszki, Z. Keresztes, S. Melikishvili, N. Ružičková, T. Hianik
Published in: "International workshop ""Novel approaches for detection proteases in milk""", Issue February 2017, 2017, Page(s) 15

High resolution ultrasonic spectroscopy in the use of sensor development for monitoring enzyme activity

Author(s): T. Szabó, B. O’Driscoll, V. Buckin, N. Molnár, Z. Keresztes
Published in: "International workshop ""Novel approaches for detection proteases in milk""", Issue February 2017, 2017, Page(s) 16

Buttermilk as carrier system for dietary supplement agents such as curcumin

Author(s): M. Pávai, B. O’Driscoll, V. Buckin, Z. Keresztes
Published in: "International workshop ""Novel approaches for detection proteases in milk""", Issue February 2017, 2017, Page(s) 17

Conduction mechanism of functionalized gold nanoparticle based electrochemical sensors

Author(s): N. Molnár, P. Juhasz, I. Tóthné-Csaba, L. Trif, Z. Keresztes, M. Donoval, M. Weis
Published in: "International workshop ""Novel approaches for detection proteases in milk""", Issue February 2017, 2017, Page(s) 18

Colief® lactase enzyme infant drops product origin and application development

Author(s): J. Byrne
Published in: "International workshop ""Recent applications of high-resolution ultrasonic spectroscopy for monitoring of hydrolytic activities in milks""", Issue November 2017, 2017, Page(s) 9

Determination of aflatoxin M1 in milk and milk samples using ELISA

Author(s): R. Sarok, A. Poturnayova, K. Szabo, A. Hucker, T. Hianik
Published in: "International workshop ""Recent applications of high-resolution ultrasonic spectroscopy for monitoring of hydrolytic activities in milks", Issue November 2017, 2017, Page(s) 10

Detection of microorganisms in food products by atomic force microscopy

Author(s): J. Nagy, Z. S. Steinerné, A. Poturnayova, A. Hucker, K. Szabo, T. Hianik
Published in: "International workshop ""Recent applications of high-resolution ultrasonic spectroscopy for monitoring of hydrolytic activities in milks""", Issue November 2017, 2017, Page(s) 12

Application of High-Resolution Ultrasonic Spectroscopy for real- time monitoring of trypsin activity in β - casein solution

Author(s): S. Melikishvili, Mark Dizon, B. O’Driscoll3, V. Buckin, T. Hianik
Published in: "International workshop ""Recent applications of high-resolution ultrasonic spectroscopy for monitoring of hydrolytic activities in milks""", Issue November 2017, 2017, Page(s) 16

Application of quartz crystal microbalance methods for casein proteolytic detection on hydrophylic silica surface

Author(s): M. Tatarko, E. Muckley, I.N. Ivanov, M. Thompson, T. Hianik
Published in: 19th Conference of Czech and Slovak Physicists Proceedings, Issue 2017, 2017, Page(s) 35-36

On the reproducibility problems of surface pressure - area isoth of bovine β-casein layers at air/water interface

Author(s): L. Romanszki, Z. Keresztes, T. Hianik
Published in: Book of Abstracts, 11th Conference on Colloid Chemistry, Issue May 28-30, 2018, 2018, Page(s) 85

The versatility of contact angle measurement

Author(s): L. Romanszki, Z. Keresztes
Published in: Book of Abstracts, 11th Conference on Colloid Chemistry, Issue May 28-30, 2018, 2018, Page(s) 110

Multi-frequency acoustic biosensor for label-free detection of self-assembled casein layer degradation by serine proteases

Author(s): M. Tatarko, E.S. Muckley, T. Hianik, I.N. Ivanov
Published in: Book of Abstracts, 11th Conference on Colloid Chemistry, Issue May 28-30, 2018, 2018, Page(s) 64

Application of high resolution ultrasonic spectroscopy (HR–US) on realtime monitoring of whey protein hydrolysis

Author(s): M. Dizon, M. Caras-Altas, V. Buckin
Published in: Book of Abstracts, Third Year PhD Talks 2018, Dublin Chemistry Graduate Seminars 2017-18, Issue 29th May, 2018, 2018

Low-cost scalable quartz crystal microbalance array for environmental sensing

Author(s): Eric S. Muckley, Cristain Anazagasty, Christopher B. Jacobs, Tibor Hianik, Ilia N. Ivanov
Published in: Organic Sensors and Bioelectronics IX, 2016, Page(s) 99440Y
DOI: 10.1117/12.2237942

Electrochemical enzyme activity assays in raw milk samples - application in a regional quality control survey

Author(s): Z. Keresztes, A. Hucker, K. Pribransky, G. Castillo, R. Kocsis, T. Hianik
Published in: "AEMIS 2016, International Workshop ""Acoustic and electrochemical methods in the study of affinity interactions at surfaces""", Issue 20 June 2016, 2016, Page(s) 18

Acoustic and AFM studies of protein cleavage by plasmin

Author(s): A. Poturnayova, I. Karpisova, G. Castillo, Z. Keresztes, T. Hianik
Published in: "AEMIS 2016, International Workshop ""Acoustic and electrochemical methods in the study of affinity interactions at surfaces""", Issue 20 June 2016, 2016, Page(s) 17

Study of plasmin activity on specific peptide substrate by photometric and electrochemical methods

Author(s): G. Castillo, Z. Keresztes, G. Mezo, A. Csampai, T. Hianik
Published in: "AEMIS 2016, International Workshop ""Acoustic and electrochemical methods in the study of affinity interactions at surfaces""", Issue 20 June 2016, 2016, Page(s) 24

Real-time electrochemical follow-up of enzyme activity in milk-based gels

Author(s): E. Orosz, G. Meszaros, Z. Fekete, A. Hucker, R. Kocsis, Z. Keresztes
Published in: "AEMIS 2016, International Workshop ""Acoustic and electrochemical methods in the study of affinity interactions at surfaces""", Issue 20 June 2016, 2016, Page(s) 25

Proteases activity detection on immobilizeed layer of casein by biosensor based on transverse shear mode transducer

Author(s): M. Tatarko, A. Poturnayova, I. Karpisova, G. Castillo, Z. Keresztes, T. Hianik
Published in: "AEMIS 2016, International Workshop ""Acoustic and electrochemical methods in the study of affinity interactions at surfaces""", Issue 20 June 2016, 2016, Page(s) 26

Structural characterization possibilities of milk-derived colloidal particles: from casein micelles towards extracellular vesicles

Author(s): J. Mihaly, A. Wacha, R. Deak, A. Bota, Z. Varga
Published in: "AEMIS 2016, International Workshop ""Acoustic and electrochemical methods in the study of affinity interactions at surfaces""", Issue 20 June 2016, 2016, Page(s) 27

Synthesis, characterization and antibacterial activity of gold nanoparticles prepared by citrus sinensis extracts

Author(s): V. Oravczova, G. Castillo, Z. Kovalova, V. Subjakova
Published in: "AEMIS 2016, International Workshop ""Acoustic and electrochemical methods in the study of affinity interactions at surfaces""", Issue 20 June 2016, 2016, Page(s) 29

SERS surfaces for bio applications

Author(s): M. Pelach, M. Sohova, M. Weis, I. Ivanov, P. Siffalovic, T. Hianik
Published in: "AEMIS 2016, International Workshop ""Acoustic and electrochemical methods in the study of affinity interactions at surfaces""", Issue 20 June 2016, 2016, Page(s) 33

Development of biosensor for detection proteases activity by means of thickness shear mode transducer with immobilised casein

Author(s): A. Poturnayova, M. Tatarko, I. Karpisova, G. Castillo, Z. Keresztes, T. Hianik
Published in: Book of contributions, 7th Slovak Biophysical Symposium, Issue April 6-8, 2016, 2016, Page(s) 96

Detection of plasmin based on specific peptide substrate and casein using acousttic transducer

Author(s): A. Poturnayova, M. Tatarko, I. Karpisova, G. Castillo, Z. Keresztes, T. Hianik
Published in: 26 Aniversary World Congress on Biosensors, Gothenburg, Sweden, Issue 25-27 May 2016, 2016, Page(s) P3.161

Casein probe–based fast plasmin determination in the picomolar range by an ultra-high frequency acoustic wave biosensor

Author(s): L. Románszki, M. Tatarko, M. Jiao, Z. Keresztes, T. Hianik, M. Thompson
Published in: Book of Abstracts, 32nd Conference of The European Colloid and Interface Society, Ljubljana, Slovenia, 2018, Page(s) 131

Advancing characterization of materials and interfaces with multi-modal, multi-scale approaches, and AI-centered analytics

Author(s): Ilia N. Ivanov, Ivan Sukuba, Eric S. Muckley, Monojoy Goswami, Tibor Hianik
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 9

The role of the H2020 project: Investigating of non-compliant dairy products based on industrial needs

Author(s): Attila Hucker, Katalin Szabó, Judit Süle, Zsófia Keresztes, Barbara Csupor, Róbert Kocsis
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 10

Development of acoustic sensor for detection chymotrypsin activity at surfaces

Author(s): I. Piovarci, A. Poturnayova, J. Nagy, R. Sarok, A. Hucker, K. Szabo, T. Hianik
Published in: BioSensor 2017,1st European and 10th German BioSensor Symposium, Issue 2017, 2017, Page(s) 218

Stability monitoring of casein layer on hydrophilic SO2 surface using quartz crystal microbalance with dissipation

Author(s): Marek Tatarko, Ilia N. Ivanov, Tibor Hianik
Published in: BioSensors 2017, 1st European and 10th German BioSensor Symposium, Potsdam, Germany, Issue 2017, 2017, Page(s) 178

Adsorption of extracellular vesicles to hydrophilic and hydrophobic surfaces

Author(s): Loránd Románszki, Judith Mihály, Zoltán Varga, Michael Thompson, Zsófia Keresztes
Published in: Book of Abstracts, 33rd Conference of The European Colloid and Interface Society, 2019, Page(s) 276

Research means business!

Author(s): J. Byrne
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 11

Real-time monitoring of enzymatic hydrolysis of milk proteins by High-Resolution Ultrasonic Spectroscopy

Author(s): Vitaly Buckin and Mark Dizon
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 12

Application of high-resolution ultrasonic spectroscopy for real-time monitoring of trypsin activity in β-casein solution

Author(s): Sopio Melikishvili, Mark Dizon, Vitaly Buckin and Tibor Hianik
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 13

Determination of lactase activity by absorption spectroscopy

Author(s): John Byrne and Marcela Morvová Jr
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 14

Determination of plasmin in milk using QCM and ELISA methods

Author(s): Alexandra Poturnayova, Katalin Szabo, Attila Hucker, Tibor Hianik
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 15

Application of acoustic methods to study proteolytic degradation of caseins by plasmin

Author(s): Marek Tatarko, Eric Muckley, Ilia N. Ivanov, Michael Thompson, Loránd Románszki, Katalin Szabo, Atilla Hucker, Tibor Hianik
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 16

Plasmin concentration measurement on interfacial beta-casein layers by means of tensiometry and EMPAS

Author(s): Loránd Románszki, Zoltán Varga, Judith Mihály, Zsófia Keresztes, Marek Tatarko, Tibor Hianik, Mengchi Jiao and Michael Thompson
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 17

Effect of surface roughness on the availability of surface confined enzyme substrates - implications for sensor responses

Author(s): Ádám Vass, István Bakos, Zsófia Keresztes, Eric S. Muckley and Ilia N. Ivanov
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 18

Biochemically directed metallic cluster formation and its application in enzymatic cleavage detection

Author(s): Tamás Szabó, Ilona Felhősi, Barbara Csupor, Mária Pávai, Zsófia Keresztes, Mark Dizon, Breda O’Driscoll, Vitaly Buckin, Itthipon Jeerapan and Joseph Wang
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 19

Development of portable potentiostat for sensing applications

Author(s): Martin Donova, Martin Weis, Ľuboš Černaj, Ján Šubjak
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 20

Development of potentiostat for specific applications: expectations, requirements, and threats

Author(s): Miroslav Novota, Michal Mičjan, Martin Weis, Martin Donoval
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 21

Participation of seconded researchers on EMPAS development

Author(s): Michal Hanic, Tomáš Debnár, Martin Weis, Martin Donoval
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 22

Development of electrochemical biosensor for detection aflatoxin M1 in a milk

Author(s): Stefanos Karapetis, Dimitrios Nikolelis and Tibor Hianik
Published in: Book of Abstracts, Advances in analytical methods for monitoring food quality and safety, 2019, Page(s) 23

Detection of plasmin activity at biomimetic surfaces using electrochemical, acoustics and AFM methods

Author(s): Tibor Hianik, Marek Tatarko, Alexandra Poturnayova, Loránd Romanszki, Zsófia Keresztes, Eric S. Muckley, Ilia N. Ivanov, Michael Thompson
Published in: Book of Abstracts, From routine to novelty. Analysis methods in dairy industry., 2018, Page(s) 12

Monitoring of plasmin regulation on immobilised milk protein using acoustic methods

Author(s): Marek Tatarko, and Tibor Hianik
Published in: Book of Abstracts, From routine to novelty. Analysis methods in dairy industry, 2019, Page(s) 22

Comparing plasmin activity measurements at the air/liquid and liquid/solid interfaces

Author(s): Loránd Románszki, Marek Tatarko, Mengchi Jiao, Zsófia Keresztes, Tibor Hianik, Michael Thompson
Published in: Book of Abstracts, From routine to novelty. Analysis methods in dairy industry., 2018, Page(s) 23

Protein stabilised nanoparticle assisted detection of proteolytic activity studied with photometric and HR-US spectroscopic methods

Author(s): Tamás Szabó, László Trif, Nikoletta Molnár, Barbara Csupor, Breda O’Driscoll, Vitaly Buckin, Zsófia Keresztes
Published in: Book of Abstracts, From routine to novelty. Analysis methods in dairy industry, 2018, Page(s) 25

The synthesis of polymer-enzyme nanoparticles and their reaction with beta-casein

Author(s): Györgyi Szarka, Márk Bisztrán, György Kasza, Tímea Stumphauser, Mark Dizon, Breda O'Driscoll, Zsófia Keresztes, Béla Iván, Vitaly Buckin
Published in: Book of Abstracts, From routine to novelty. Analysis methods in dairy industry, 2018, Page(s) 31

Tunable size gold nanoparticle-enzyme substrate conjugates: posible sensor applications

Author(s): T. Szabo, B. Csupor, P. Pekker, Z. Keresztes
Published in: Book of Abstracts, Matrafured 2019, International Conference on Chemical Sensors, Visegrad, Hungary, 2019, Issue June 16-21, 2019, 2019, Page(s) 176

Application of acoustic methods for detection of casein degradation caused by milk protease systems

Author(s): M. Tatarko, L. Romanszki, E.S. Muckley, I.N. Ivanov, M. Thompson, T. Hianik
Published in: Book of Contributions, 11th International Conference Structure and Stability of Biomacromolecules, SSB2019, September 3 – 6, 2019, Košice, Slovakia, 2019, Page(s) 64-65