A synthetic biology approach for magnetization of foreign organisms by genetic engineering and transplantation of bacterial magnetosome biosynthesis

Project Information

SYNTOMAGX

Grant agreement ID: 692637

DOI

10.3030/692637

Project closed

EC signature date 15 June 2016

Start date 1 September 2016

End date 31 August 2022

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 2 291 875,00

EU contribution

€ 2 291 875,00

Coordinated by

UNIVERSITAT BAYREUTH
Germany

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Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (31)

Bacterioferritin of Magnetospirillum gryphiswaldense Is a Heterotetraeicosameric Complex Composed of Functionally Distinct Subunits but Is Not Involved in Magnetite Biomineralization

Author(s): René Uebe, Frederik Ahrens, Jörg Stang, Katharina Jäger, Lars H. Böttger, Christian Schmidt, Berthold F. Matzanke, Dirk Schüler
Published in: mBio, Issue 10/3, 2019, Page(s) e02795-18, ISSN 2150-7511
Publisher: American Society for Microbiology
DOI: 10.1128/mbio.02795-18

A bacterial cytolinker couples positioning of magnetic organelles to cell shape control

Author(s): Daniel Pfeiffer, Mauricio Toro-Nahuelpan, Ram Prasad Awal, Frank-Dietrich Müller, Marc Bramkamp, Jürgen M. Plitzko, Dirk Schüler
Published in: Proceedings of the National Academy of Sciences, Issue 117/50, 2020, Page(s) 32086-32097, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2014659117

Biocompatibility, uptake and subcellular localization of bacterial magnetosomes in mammalian cells.

Author(s): Frank Mickoleit; Cornelia Jörke; Stefan Geimer; Denis S. Maier; Jörg P. Müller; Johanna Demut; Christine Gräfe; Dirk Schüler; Joachim H. Clement
Published in: Nanoscale Advances, Issue 3, 2021, Page(s) 3799, ISSN 2516-0230
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0na01086c

The transcriptomic landscape of Magnetospirillum gryphiswaldense during magnetosome biomineralization

Author(s): Cornelius N. Riese, Manuel Wittchen, Valérie Jérôme, Ruth Freitag, Tobias Busche, Jörn Kalinowski, and Dirk Schüler
Published in: BMC Genomics, Issue 23, 2022, Page(s) 699, ISSN 1471-2164
Publisher: BioMed Central
DOI: 10.1186/s12864-022-08913-x

Towards a 'chassis' for bacterial magnetosome biosynthesis: Genome streamlining of  Magnetospirillum gryphiswaldense by multiple deletions

Author(s): Theresa Zwiener, Marina V. Dziuba, Frank Mickoleit, Christian Rückert, Tobias Busche, Jörn Kalinowski, Rene Uebe, and Dirk Schüler
Published in: Microbial Cell Factories, Issue 20, 2021, Page(s) 35, ISSN 1475-2859
Publisher: BioMed Central
DOI: 10.1186/s12934-021-01517-2

Genome-Wide Identification of Essential and Auxiliary Gene Sets for Magnetosome Biosynthesis in Magnetospirillum gryphiswaldense

Author(s): Karen T. Silva, Margarete Schüler, Frank Mickoleit, Theresa Zwiener, Frank D. Müller, Ram Prasad Awal, Alfons Weig, Andreas Brachmann, René Uebe, Dirk Schüler
Published in: mSystems, Issue 5/6, 2020, Page(s) e00565-20, ISSN 2379-5077
Publisher: ASM
DOI: 10.1128/msystems.00565-20

Quantifying the benefit of a dedicated 'magnetoskeleton' in bacterial magnetotaxis by live-cell motility tracking and soft agar swimming assay

Author(s): Daniel Pfeiffer and Dirk Schüler
Published in: Applied and Environmental Microbiology, Issue 86/3, 2020, Page(s) e01976-19, ISSN 0099-2240
Publisher: American Society for Microbiology
DOI: 10.1128/aem.01976-19

In vivo Architecture of the Polar Organizing Protein Z (PopZ) Meshwork in the Alphaproteobacteria Magnetospirillum gryphiswaldense and Caulobacter crescentus

Author(s): Mauricio Toro-Nahuelpan; Jürgen M. Plitzko; Dirk Schüler; Daniel Pfeiffer
Published in: Journal of Molecular Biology, Issue 434, 2022, Page(s) 167423, ISSN 0022-2836
Publisher: Academic Press
DOI: 10.1016/j.jmb.2021.167423

A Magnetosome-Based Platform for Flow Biocatalysis

Author(s): Esther Mittmann, Frank Mickoleit, Denis S. Maier, Sabrina Y. Stäbler, Marius A. Klein, Christof M. Niemeyer, Kersten S. Rabe, and Dirk Schüler
Published in: ACS Applied Materials & Interfaces, Issue 14, 2022, Page(s) 22138-22150, ISSN 1944-8252
Publisher: American Chemical Society
DOI: 10.1021/acsami.2c03337

The Complex Transcriptional Landscape of Magnetosome Gene Clusters in Magnetospirillum gryphiswaldense

Author(s): Marina Dziuba, Cornelius N. Riese, Manuel Wittchen, Tobias Busche, Jörn Kalinowski, René Uebe, and Dirk Schüler
Published in: mSystems, Issue 6/5, 2021, Page(s) e00893-21, ISSN 2379-5077
Publisher: American Society for Microbiology
DOI: 10.1128/msystems.00893-21

Induction of Axonal Outgrowth in Mouse Hippocampal Neurons via Bacterial Magnetosomes.

Author(s): Sara De Vincentiis; Alessandro Falconieri; Frank Mickoleit; Valentina Cappello; Dirk Schüler; Vittoria Raffa
Published in: International Journal of Molecular Sciences, Issue 22/8, 2021, Page(s) 4126, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22084126

Identification and elimination of genomic regions irrelevant for magnetosome biosynthesis by large-scale deletion in Magnetospirillum gryphiswaldense

Author(s): Theresa Zwiener, Frank Mickoleit, Marina V. Dziuba, Christian Rückert, Tobias Busche, Jörn Kalinowski, Damien Faivre, Rene Uebe, and Dirk Schüler
Published in: BMC Microbiology, Issue 21, 2021, Page(s) 65, ISSN 1471-2180
Publisher: BioMed Central
DOI: 10.1186/s12866-021-02124-2

A Versatile Toolkit for Controllable and Highly Selective Multifunctionalization of Bacterial Magnetic Nanoparticles

Author(s): Frank Mickoleit, Clarissa Lanzloth, and Dirk Schüler
Published in: Small, Issue 16, 2020, Page(s) 1906922, ISSN 1613-6829
Publisher: Wiley
DOI: 10.1002/smll.201906922

Probing the nanostructure and arrangement of bacterial magnetosomes by small-angle X-ray scattering

Author(s): Sabine Rosenfeldt, Cornelius Riese, Frank Mickoleit, Dirk Schüler, and Anna Schenk
Published in: Applied and Environmental Microbiology, Issue 85/24, 2019, Page(s) e01513-19, ISSN 0099-2240
Publisher: American Society for Microbiology
DOI: 10.1128/aem.01513-19

Bacterial Magnetosomes as Novel Platform for the Presentation of Immunostimulatory, Membrane-bound Ligands in Cellular Biotechnology

Author(s): Frank Mickoleit, Valérie Jérôme, Ruth Freitag, and Dirk Schüler
Published in: Advanced Biosystems, Issue 4, 2020, Page(s) 1900231, ISSN 2366-7478
Publisher: Wiley
DOI: 10.1002/adbi.201900231

An automated oxystat fermentation regime for microoxic cultivation of Magnetospirillum gryphiswaldense

Author(s): Cornelius N. Riese, René Uebe, Sabine Rosenfeldt, Anna S. Schenk, Valérie Jérôme, Ruth Freitag, Dirk Schüler
Published in: Microbial Cell Factories, Issue 19/1, 2020, Page(s) 206, ISSN 1475-2859
Publisher: BioMed Central
DOI: 10.1186/s12934-020-01469-z

Magnetosome proteins belong to universal protein families involved in many cell processes

Author(s): Caroline L. Monteil, David Vallenet, Dirk Schüler, and Christopher T. Lefevre
Published in: Proceedings of the National Academy of Sciences, Issue 119/39, 2022, Page(s) e2208648119, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2208648119

Sesbanimide R, a Novel Cytotoxic Polyketide Produced by Magnetotactic Bacteria

Author(s): Ram Prasad Awal, Patrick A. Haack, Chantal D. Bader, Cornelius N. Riese, Dirk Schüler, and Rolf Müller
Published in: mBio, Issue 12/3, 2021, Page(s) e00591-21, ISSN 2150-7511
Publisher: American Society for Microbiology
DOI: 10.1101/2020.12.22.424014

In Vivo Coating of Bacterial Magnetic Nanoparticles by Magnetosome Expression of Spider Silk-Inspired Peptides

Author(s): Frank Mickoleit, Christian B. Borkner, Mauricio Toro-Nahuelpan, Heike M. Herold, Denis S. Maier, Jürgen M. Plitzko, Thomas Scheibel, Dirk Schüler
Published in: Biomacromolecules, Issue 19/3, 2018, Page(s) 962-972, ISSN 1525-7797
Publisher: American Chemical Society
DOI: 10.1021/acs.biomac.7b01749

Generation of nanomagnetic biocomposites by genetic engineering of bacterial magnetosomes

Author(s): Frank Mickoleit, Dirk Schüler
Published in: Bioinspired, Biomimetic and Nanobiomaterials, Issue 8/1, 2019, Page(s) 86-98, ISSN 2045-9866
Publisher: Thomas Telford Ltd
DOI: 10.1680/jbibn.18.00005

Generation of Multifunctional Magnetic Nanoparticles with Amplified Catalytic Activities by Genetic Expression of Enzyme Arrays on Bacterial Magnetosomes

Author(s): Frank Mickoleit, Dirk Schüler
Published in: Advanced Biosystems, Issue 2/1, 2018, Page(s) 1700109, ISSN 2366-7478
Publisher: Wiley-VCH Verlag GmbH & Co. KGaA
DOI: 10.1002/adbi.201700109

The Polar Organizing Protein PopZ Is Fundamental for Proper Cell Division and Segregation of Cellular Content in Magnetospirillum gryphiswaldense

Author(s): Daniel Pfeiffer, Mauricio Toro-Nahuelpan, Marc Bramkamp, Jürgen M. Plitzko, Dirk Schüler
Published in: mBio, Issue 10/2, 2019, ISSN 2150-7511
Publisher: American Society for Microbiology
DOI: 10.1128/mbio.02716-18

The dual role of MamB in magnetosome membrane assembly and magnetite biomineralization

Author(s): René Uebe, Noa Keren-Khadmy, Natalie Zeytuni, Emanuel Katzmann, Yotam Navon, Geula Davidov, Ronit Bitton, Jürgen M. Plitzko, Dirk Schüler, Raz Zarivach
Published in: Molecular Microbiology, Issue 107/4, 2018, Page(s) 542-557, ISSN 0950-382X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/mmi.13899

A quantitative assessment of the membrane-integral sub-proteome of a bacterial magnetic organelle

Author(s): Oliver Raschdorf, Florian Bonn, Natalie Zeytuni, Raz Zarivach, Dörte Becher, Dirk Schüler
Published in: Journal of Proteomics, Issue 172, 2018, Page(s) 89-99, ISSN 1874-3919
Publisher: Elsevier BV
DOI: 10.1016/j.jprot.2017.10.007

Towards standardized purification of bacterial magnetic nanoparticles for future in vivo applications

Author(s): Sabine Rosenfeldt, Frank Mickoleit, Cornelia Jörke, Joachim H. Clement, Simon Markert, Valerie Jérôme, Stephan Schwarzinger, Ruth Freitag, Dirk Schüler, Rene Uebe, and Anna S. Schenk
Published in: Acta Biomaterialia, Issue 120, 2020, Page(s) 293-303, ISSN 1742-7061
Publisher: Elsevier BV
DOI: 10.1016/j.actbio.2020.07.042

A gradient-forming MipZ protein mediating the control of cell division in the magnetotactic bacterium Magnetospirillum gryphiswaldense

Author(s): Mauricio Toro-Nahuelpan, Laura Corrales-Guerrero, Theresa Zwiener, Manuel Osorio-Valeriano, Frank-Dietrich Müller, Jürgen M. Plitzko, Marc Bramkamp, Martin Thanbichler, and Dirk Schüler
Published in: Molecular Microbiology, Issue 112/5, 2019, Page(s) 1423-1439, ISSN 0950-382X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/mmi.14369

High-Yield Production, Characterization, and Functionalization of Recombinant Magnetosomes in the Synthetic Bacterium Rhodospirillum rubrum “magneticum”

Author(s): Frank Mickoleit, Sabine Rosenfeldt, Mauricio Toro-Nahuelpan, Miroslava Schaffer, Anna S. Schenk, Jürgen M. Plitzko, Dirk Schüler
Published in: Advanced Biology, Issue 5/9, 2021, Page(s) 2101017, ISSN 2701-0198
Publisher: Wiley
DOI: 10.1002/adbi.202101017

A Compass To Boost Navigation: Cell Biology of Bacterial Magnetotaxis

Author(s): Frank D. Müller, Dirk Schüler, Daniel Pfeiffer
Published in: Journal of Bacteriology, Issue 202/21, 2020, Page(s) e00398-20, ISSN 0021-9193
Publisher: American Society for Microbiology
DOI: 10.1128/jb.00398-20

Single‐step transfer of biosynthetic operons endows a non‐magnetotactic Magnetospirillum strain from wetland with magnetosome biosynthesis

Author(s): Marina V. Dziuba, Theresa Zwiener, Rene Uebe, Dirk Schüler
Published in: Environmental Microbiology, Issue 22/4, 2020, Page(s) 1603-1618, ISSN 1462-2912
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.14950

Spatiotemporal Organization of Chemotaxis Pathways in Magnetospirillum gryphiswaldense

Author(s): Daniel Pfeiffer, Julian Herz, Julia Schmiedel, Felix Popp, Dirk Schüler
Published in: Applied and Environmental Microbiology, Issue 87/1, 2020, Page(s) e02229-20, ISSN 0099-2240
Publisher: American Society for Microbiology
DOI: 10.1128/aem.02229-20

MamY is a membrane-bound protein that aligns magnetosomes and the motility axis of helical magnetotactic bacteria

Author(s): Mauricio Toro-Nahuelpan, Giacomo Giacomelli, Oliver Raschdorf, Sarah Borg, Jürgen M. Plitzko, Marc Bramkamp, Dirk Schüler, Frank-Dietrich Müller
Published in: Nature Microbiology, Issue 4/11, 2019, Page(s) 1978-1989, ISSN 2058-5276
Publisher: Springer
DOI: 10.1038/s41564-019-0512-8