Eliminating Oxygen Requirements in Yeasts

Project Information

ELOXY

Grant agreement ID: 694633

DOI 10.3030/694633

Project closed

EC signature date 20 July 2016

Start date 1 September 2016

End date 31 August 2021

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 498 150,00

EU contribution € 2 498 150,00

Coordinated by

TECHNISCHE UNIVERSITEIT DELFT
Netherlands

Publications

Peer reviewed articles (11)

Engineering heterologous molybdenum-cofactor-biosynthesis and nitrate-assimilation pathways enables nitrate utilization by Saccharomyces cerevisiae

Author(s): Thomas Perli, Daan N.A. van der Vorm, Mats Wassink, Marcel van den Broek, Jack T. Pronk, Jean-Marc Daran
Published in: Metabolic Engineering, Issue 65, 2021, Page(s) 11-29, ISSN 1096-7184
Publisher: Elsevier
DOI: 10.1016/j.ymben.2021.02.004

A squalene–hopene cyclase in Schizosaccharomyces japonicus represents a eukaryotic adaptation to sterol-limited anaerobic environments

Author(s): Jonna Bouwknegt, Sanne J. Wiersma, Raúl A. Ortiz-Merino, Eline S. R. Doornenbal, Petrik Buitenhuis, Martin Giera, Christoph Müller, Jack T. Pronk
Published in: Proceedings of the National Academy of Sciences, Issue 118/32, 2021, Page(s) e2105225118, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2105225118

Squalene-Tetrahymanol Cyclase Expression Enables Sterol-Independent Growth of Saccharomyces cerevisiae

Author(s): Sanne J. Wiersma, Christiaan Mooiman, Martin Giera, Jack T. Pronk
Published in: Applied and Environmental Microbiology, Issue 86/17, 2020, ISSN 0099-2240
Publisher: American Society for Microbiology
DOI: 10.1128/aem.00672-20

Critical parameters and procedures for anaerobic cultivation of yeasts in bioreactors and anaerobic chambers

Author(s): Christiaan Mooiman, Jonna Bouwknegt, Wijb J C Dekker, Sanne J Wiersma, Raúl A Ortiz-Merino, Erik de Hulster, Jack T Pronk
Published in: FEMS Yeast Research, Issue 21/5, 2021, ISSN 1567-1364
Publisher: Oxford Academic
DOI: 10.1093/femsyr/foab035

Anaerobic growth of Saccharomyces cerevisiae CEN.PK113-7D does not depend on synthesis or supplementation of unsaturated fatty acids

Author(s): Wijb J C Dekker, Sanne J Wiersma, Jonna Bouwknegt, Christiaan Mooiman, Jack T Pronk
Published in: FEMS Yeast Research, Issue 19/6, 2019, ISSN 1567-1356
Publisher: Blackwell
DOI: 10.1093/femsyr/foz060

Identification of Oxygen-Independent Pathways for Pyridine Nucleotide and Coenzyme A Synthesis in Anaerobic Fungi by Expression of Candidate Genes in Yeast

Author(s): Thomas Perli, Aurin M. Vos, Jonna Bouwknegt, Wijb J. C. Dekker, Sanne J. Wiersma, Christiaan Mooiman, Raúl A. Ortiz-Merino, Jean-Marc Daran, Jack T. Pronk
Published in: mBio, Issue 12/3, 2021, ISSN 2150-7511
Publisher: American Society for Microbiology
DOI: 10.1128/mbio.00967-21

Engineering the thermotolerant industrial yeast Kluyveromyces marxianus for anaerobic growth

Author(s): Wijbrand J.C. Dekker, Raúl A. Ortiz-Merino, Astrid Kaljouw, Julius Battjes, Frank W. Wiering, Christiaan Mooiman, Pilar de la Torre, Jack T. Pronk
Published in: Metabolic Engineering, Issue 67, 2021, Page(s) 347-364, ISSN 1096-7176
Publisher: Academic Press
DOI: 10.1016/j.ymben.2021.07.006

Vitamin requirements and biosynthesis in Saccharomyces cerevisiae

Author(s): Thomas Perli, Anna K. Wronska, Raúl A. Ortiz‐Merino, Jack T. Pronk, Jean‐Marc Daran
Published in: Yeast, Issue 37/4, 2020, Page(s) 283-304, ISSN 0749-503X
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/yea.3461

Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid

Author(s): Hannes Juergens, Javier A Varela, Arthur R Gorter de Vries, Thomas Perli, Veronica J M Gast, Nikola Y Gyurchev, Arun S Rajkumar, Robert Mans, Jack T Pronk, John P Morrissey, Jean-Marc G Daran
Published in: FEMS Yeast Research, Issue 18/3, 2018, ISSN 1567-1364
Publisher: Oxford Academic
DOI: 10.1093/femsyr/foy012

Under pressure: evolutionary engineering of yeast strains for improved performance in fuels and chemicals production

Author(s): Robert Mans, Jean-Marc G Daran, Jack T Pronk
Published in: Current Opinion in Biotechnology, Issue 50, 2018, Page(s) 47-56, ISSN 0958-1669
Publisher: Elsevier BV
DOI: 10.1016/j.copbio.2017.10.011

Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis

Author(s): Jonna Bouwknegt, Charlotte C. Koster, Aurin M. Vos, Raúl A. Ortiz-Merino, Mats Wassink, Marijke A. H. Luttik, Marcel van den Broek, Peter L. Hagedoorn, Jack T. Pronk
Published in: Fungal Biology and Biotechnology, Issue 8/1, 2021, ISSN 2054-3085
Publisher: Springer Nature
DOI: 10.1186/s40694-021-00117-4

Thesis and dissertations (2)

Identification and elimination of biosynthetic oxygen requirement in yeasts

Author(s): W.J.C. Dekker
Published in: 2021, ISBN 978-94-6423-422-0
Publisher: Delft University of Technology
DOI: 10.4233/uuid:fd04588a-842b-4851-959e-4f1d24fd0bc3

Oxygen requirements for lipid synthesis in yeasts

Author(s): S.J. Wiersma
Published in: 2021, ISBN 978-94-6384-239-6
Publisher: Delft University of Technology
DOI: 10.4233/uuid:e0a82278-daa1-4ea3-ad33-dc18b825be90

Intellectual Property Rights

Patent (2)

RECOMBINANT FUNGAL CELL

Application/Publication number: 20 20050818
Date: 2020-12-24

BIOTIN PROTOTROPHY

Application/Publication number: 20 20063762
Date: 2020-05-18