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Structural investigation into functionality of Chikungunya virus nsP2 in replication complex and screening of small molecules for its inhibition

Objective

Chikungunya virus (CHIKV) is a medically important pathogen that affected millions of people around the world. CHIKV
infection causes high fever, intense joint pain and often leads to chronic virus-induced arthritis, thus there is a clear necessity
to develop antiviral compounds capable of eliminating persisting virus. CHIKV is an alphavirus from Togaviridae family with
single-stranded positive-sense RNA genome, which replication is carried out by a complex of four viral non-structural (ns)
proteins, initially produced in a form of ns-polyprotein precursor and released by proteolytic processing. Because
multifunctional nsP2 plays a central role in a replication cycle of CHIKV by exhibiting various enzymatic activities and
counteracting cellular defence mechanisms, it represents the most attractive target for drug design and screening. Currently,
the structural information regarding CHIKV ns-proteins is limited, whereas the platforms for in vitro testing the potential
inhibitors are largely non-existent. Therefore the first aims of this project will be to apply structural biology approaches to
elucidate spatial organization of nsP2 and to reveal its contacts with other ns-proteins in the replication complex.
The druggability of nsP2 will be then explored by screening recombinant proteins against libraries of chemical fragments and
characterizing binding of small molecules using sensitive biophysical techniques and crystallographic analysis. The potential
of selected compounds to interfere with enzymatic or interaction properties of nsP2 will be revealed using variety of
fluorescence-based assays developed in this project. The newly discovered knowledge about structural features of CHIKV
nsP2, identification of novel protein surface pockets and binding molecules, and the development of functional assays for the
analysis of their inhibitory potential are expected to create the ground for future elaboration of novel specific inhibitors of
CHIKV.

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Programme(s)

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Topic(s)

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MSCA-IF-EF-ST - Standard EF

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2015

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Coordinator

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 195 454,80
Address
TRINITY LANE THE OLD SCHOOLS
CB2 1TN CAMBRIDGE
United Kingdom

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Region
East of England East Anglia Cambridgeshire CC
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 195 454,80
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