Objective
Water, oxygen, and sunlight are essential to most forms of life, yet they also represent a challenging environment for the genetic material as they drive reactions that lead to damage of DNA. To ensure cell survival, an elaborate response to DNA damage maintains genome integrity. Until recently, the DNA damage response (DDR) was described as a wave of protein posttranslational modifications that signal the presence of DNA damage to the cell and mobilize enzymatic repair pathways. Recently, genome-wide screens and advances in next-generation sequencing (NGS) shed light on the role of RNA-binding proteins and non-coding RNAs (ncRNAs) in the DDR. A novel class of small ncRNAs produced from the vicinity of DNA double strand breaks (DSB) – DNA damage-inducible ncRNAs (diRNAs) - were identified in plants, flies, and mammals. Our understanding of the biogenesis of diRNAs and their role in DSB repair is currently limited by the lack of methods for their detection that can be implemented in every laboratory. The aim of this project is to close this technological gap by developing robust and versatile methods of analysis of diRNA biogenesis. Our original approach consists to map diRNAs using NGS technology at site-specific DSBs inducible by an endonuclease, and to create the first database of human diRNAs. The knowledge of the sequences of diRNAs will allow us to develop methods for detection of both precursor and mature diRNAs. To identify novel players of the diRNA biogenesis pathway, we will perform a small-scale siRNA-based screen of RNA-binding proteins using the developed methods. This study will lead to important technological and knowledge-based developments in the DDR field, and to a better understanding of how the “non-coding” genome regulates important cellular functions.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences genetics RNA
- natural sciences biological sciences zoology mammalogy
- natural sciences biological sciences genetics genomes
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2015
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
H91 Galway
Ireland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.