Periodic Reporting for period 1 - MF-Synapse (Presynaptic calcium channels distribution and impact on coupling at the hippocampal mossy fiber synapse)
Période du rapport: 2017-01-01 au 2018-12-31
Such depletion of docked vesicles suggests that docked vesicles represent the releasable vesicle pool and therefore that docked vesicles can be used as a structural correlate for the readily-relesable pool (RRP) of vesicles. We however noticed that after this depleting-protocol, some vesicles remained docked. We interpret this result in two fold. Firstly, this could indicate that not all docked vesicles are part of the RRP, although the majority of them are. However this could also indicate that at some point during the stimulus, rapid recruitment and replenishment of the docked vesicle pool started to happen. Thus, this method now also allows for future studies to investigate kinetics of vesicle recruitment and replenishment at the active zone, by looking at different time points after evoked synaptic transmission, with different levels of activity.