Periodic Reporting for period 4 - CaBiS (Chemistry and Biology in Synergy - Studies of hydrogenases using a combination of synthetic chemistry and biological tools )
Período documentado: 2021-08-01 hasta 2022-01-31
1) We have verified that it is possible to introduce synthetic components into the assembly line of the catalytic cofactor, and that these synthetic building blocks are practically indistinguishable from their natural, biological, counterparts. This paves the way for more detailed studies of this so-far incompletely understood process; and we have indeed also provided molecular level insight into how the biologically unique metal centre of [FeFe] hydrogenase is assembled.
2) We have shown that we can take advantage of biological tools for the construction of a new class of completely synthetic miniaturized hydrogenases. These small molecule systems represent a new promising class of manmade catalysts for hydrogen gas production.
The aforementioned work has been published in a series of manuscripts (available free of charge to the general public) as well as presented at scientific conferences.
3) We have also developed techniques for generating artificial enzymes inside living organisms, using a combination of molecular biology and synthetic chemistry tools. This has allowed us to generate fully functional artificial enzymes in both E. coli and different cyanobacteria, thereby improving their hydrogen gas production. Along the same lines, we have utilized this as a tool for screening new [FeFe] hydrogenases, which has allowed us to discover enzymes with novel properties and verified the presence of [FeFe] hydrogenase in branches of life where it was previously not thought to exist.
4) We have utilized our unique competence in constructing artificial hydrogenases inside living cells to study in great detail how these molecular machines function in a living organism.
These results have also been published in a series of manuscript (available free of charge to the general public), and also presented to the general public via (Swedish) public radio interviews and magazine publications.