Periodic Reporting for period 2 - Stress Imaging (Nanoscale Stress Imaging with Imperfect Diamonds)
Reporting period: 2018-09-01 to 2020-02-29
So far we have performed T1 measurements on yeasts and macrophages as well as reference samples. In macrophages we were able to follow nitric oxide signaling. There we are able to measure triggering and inhibition of free radical production. We also have already achieved to gain some control over the particle location within the cell by attaching antibodies to the diamond surface. So far co-localisation with the nucleus or with mitochondria has been shown.
In yeast cells we were able to improve diamond uptake and to diffententiate between young and old cells, between different knock-out strains. Additionally, we compared cells that were aged in presence or absence of an antioxidant.
The measurements on test samples helped us to identify which particles and which data analysis method are best to use . Additionally, we could learn from this data what our signals mean.
We have implemented 3D particle tracking was essential record a signal at all from a particle that is moving. However, particle trajectories give interesting additional insights. How fast a particle is moving inside the cell can for instance indicate where within a cell it is. Additionally, one can use the natural movement to obtain information on different parts of the cell. On our way to differentiating between radicals we have adapted the measuring program for pulsing and upgraded the equipment in the group.
For all the experiments that we have done with our technique we have also compared with the closest standard techniques.