Periodic Reporting for period 2 - IlluMitoDNA (Illuminating the mechanisms of mitochondrial DNA quality control and inheritance)
Reporting period: 2019-02-01 to 2020-07-31
Quite surprisingly, fundamental questions regarding the biology of mtDNA are currently poorly understood: How is mtDNA faithfully passed on to daughter cells during cell division? How are mtDNA copies distributed throughout the mitochondria, that form a tubular and reticulated network in cells? How is the integrity of mtDNA maintained over generations, despite an elevated level of reactive oxygen species within mitochondria? It is these mysteries that we aim to unravel in this project, down to a deep mechanistic understanding. The answers to these question also hold the key for a better understanding of human mitochondrial disorders and will pave the way for the development of new therapeutical approaches. To study mitochondria and mtDNA, we combine unique experimental advantages in the model organism S. cerevisiae with a wide variety of cell biological techniques, including fluorescent live-cell and super-resolution microscopy, next-generation sequencing, protein biochemistry and yeast genetics.