Objective
Chromatin regulators adjust genome functions by modifying chromatin states. Comprehensive characterization of chromatin states, associations of chromatin regulators, and expression profiles is crucial for investigating: 1.Cellular differentiation triggers. 2.The nature of the underlying chromatin regulatory mechanisms. 3.The effect of cellular heterogeneity, and 4. How chromatin affects mRNA expression. Mapping chromatin states by sequencing immunoprecipitated chromatin (ChIP-seq) provides an extraordinary resource for studying cellular states. However, a major shortcoming is that the profiles reflect a composite average over the studied cell population, concealing important information about the underlying subpopulations. To address this hurdle, I pioneered Drop-Seq, a novel drop based microfluidic technology for single cell ChIP-seq and RNA-seq. Applying the technique to thousands of embryonic stem (ES) cells, we identified a spectrum of sub-populations defined by differences in chromatin signatures of pluripotency and differentiation priming. However, despite initial progress, the extent and significance of chromatin-state heterogeneity and its relationship with mRNA expression remain largely uncharted. The central aim of this proposal is to develop a novel framework to systematically characterize cell-to-cell variability at the epigenomic level. Our approach will include the development of technology enabling ChIP-seq and RNA-seq on the same cell. We will also exploit drop-based microfluidics to develop a robust CRISPR/Cas9-based approach to assess single and multiple perturbations. Finally, we will apply these innovative technologies to questions about cellular heterogeneity and epigenomic regulation during early differentiation of ES cells. This proposal will reveal the function and interplay between chromatin regulators, histone marks and transcription events, and shed light on the underlying regulation leading to cell fate decisions.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences classical mechanics fluid mechanics microfluidics
- natural sciences biological sciences genetics genomes
- medical and health sciences medical biotechnology cells technologies
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
ERC-STG - Starting Grant
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2016-STG
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
91904 JERUSALEM
Israel
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.