Unravelling the architecture and the cartography of the human centriole

Project Information

ACCENT

Grant agreement ID: 715289

DOI 10.3030/715289

Project closed

EC signature date 25 October 2016

Start date 1 January 2017

End date 31 December 2021

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 1 498 965,00

EU contribution € 1 498 965,00

Coordinated by

UNIVERSITE DE GENEVE
Switzerland

Publications

Book chapters (3)

Improving the resolution of fluorescence nanoscopy using post-expansion labeling microscopy

Author(s): Virginie Hamel, Paul Guichard
Published in: Expansion Microscopy for Cell Biology, Issue 161, 2021, Page(s) 297-315, ISBN 9780128208076
Publisher: Elsevier
DOI: 10.1016/bs.mcb.2020.07.002

The centriolar tubulin code

Author(s): Paul Guichard; Marine H. Laporte; Virginie Hamel
Published in: Seminars in Cell & Developmental Biology, 2021, ISSN 1084-9521
Publisher: Academic Press
DOI: 10.1016/j.semcdb.2021.12.001

Ultrastructure expansion microscopy (U-ExM)

Author(s): Davide Gambarotto, Virginie Hamel, Paul Guichard
Published in: Expansion Microscopy for Cell Biology, Issue 161, 2021, Page(s) 57-81, ISBN 9780128208076
Publisher: Elsevier
DOI: 10.1016/bs.mcb.2020.05.006

Peer reviewed articles (13)

Essential function of the alveolin network in the subpellicular microtubules and conoid assembly in Toxoplasma gondii

Author(s): Nicolò Tosetti, Nicolas Dos Santos Pacheco, Eloïse Bertiaux, Bohumil Maco, Lorène Bournonville, Virginie Hamel, Paul Guichard, Dominique Soldati-Favre
Published in: eLife, Issue 9, 2020, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.56635

Expansion microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid

Author(s): Eloïse Bertiaux, Aurélia C. Balestra, Lorène Bournonville, Vincent Louvel, Bohumil Maco, Dominique Soldati-Favre, Mathieu Brochet, Paul Guichard, Virginie Hamel
Published in: PLOS Biology, Issue 19/3, 2021, Page(s) e3001020, ISSN 1545-7885
Publisher: Public Library of Science
DOI: 10.1371/journal.pbio.3001020

WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity

Author(s): Emmanuelle Steib, Marine H Laporte, Davide Gambarotto, Natacha Olieric, Celine Zheng, Susanne Borgers, Vincent Olieric, Maeva Le Guennec, France Koll, Anne-Marie Tassin, Michel O Steinmetz, Paul Guichard, Virginie Hamel
Published in: eLife, Issue 9, 2020, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.57205

Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)

Author(s): Fabian U. Zwettler, Sebastian Reinhard, Davide Gambarotto, Toby D. M. Bell, Virginie Hamel, Paul Guichard, Markus Sauer
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-17086-8

Overview of the centriole architecture

Author(s): Maeva LeGuennec, Nikolai Klena, Gabriel Aeschlimann, Virginie Hamel, Paul Guichard
Published in: Current Opinion in Structural Biology, Issue 66, 2021, Page(s) 58-65, ISSN 0959-440X
Publisher: Elsevier BV
DOI: 10.1016/j.sbi.2020.09.015

A helical inner scaffold provides a structural basis for centriole cohesion

Author(s): Maeva Le Guennec, Nikolai Klena, Davide Gambarotto, Marine H. Laporte, Anne-Marie Tassin, Hugo van den Hoek, Philipp S. Erdmann, Miroslava Schaffer, Lubomir Kovacik, Susanne Borgers, Kenneth N. Goldie, Henning Stahlberg, Michel Bornens, Juliette Azimzadeh, Benjamin D. Engel, Virginie Hamel, Paul Guichard
Published in: Science Advances, Issue 6/7, 2020, Page(s) eaaz4137, ISSN 2375-2548
Publisher: Science Advances
DOI: 10.1126/sciadv.aaz4137

Super-resolution microscopy to decipher multi-molecular assemblies

Author(s): Christian Sieben, Kyle M Douglass, Paul Guichard, Suliana Manley
Published in: Current Opinion in Structural Biology, Issue 49, 2018, Page(s) 169-176, ISSN 0959-440X
Publisher: Elsevier BV
DOI: 10.1016/j.sbi.2018.03.017

Imaging cellular ultrastructures using expansion microscopy (U-ExM)

Author(s): Davide Gambarotto, Fabian U. Zwettler, Maeva Le Guennec, Marketa Schmidt-Cernohorska, Denis Fortun, Susanne Borgers, Jörn Heine, Jan-Gero Schloetel, Matthias Reuss, Michael Unser, Edward S. Boyden, Markus Sauer, Virginie Hamel, Paul Guichard
Published in: Nature Methods, Issue 16/1, 2019, Page(s) 71-74, ISSN 1548-7091
Publisher: Nature Publishing Group
DOI: 10.1038/s41592-018-0238-1

Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails

Author(s): Marketa Schmidt-Cernohorska, Ilia Zhernov, Emmanuelle Steib, Maeva Le Guennec, Rim Achek, Susanne Borgers, Davide Demurtas, Liliane Mouawad, Zdenek Lansky, Virginie Hamel, Paul Guichard
Published in: Science, Issue 363/6424, 2019, Page(s) 285-288, ISSN 0036-8075
Publisher: American Association for the Advancement of Science

Isolation and Fluorescence Imaging for Single-particle Reconstruction of <em>Chlamydomonas</em> Centrioles

Author(s): Nikolai Klena, Davide Gambarotto, Maeva Le Guennec, Susanne Borgers, Paul Guichard, Virginie Hamel
Published in: Journal of Visualized Experiments, Issue 139, 2018, ISSN 1940-087X
Publisher: MYJoVE Corporation
DOI: 10.3791/58109

Homogeneous multifocal excitation for high-throughput super-resolution imaging

Author(s): Dora Mahecic, Davide Gambarotto, Kyle M. Douglass, Denis Fortun, Niccoló Banterle, Khalid A. Ibrahim, Maeva Le Guennec, Pierre Gönczy, Virginie Hamel, Paul Guichard, Suliana Manley
Published in: Nature Methods, Issue 17/7, 2020, Page(s) 726-733, ISSN 1548-7091
Publisher: Nature Publishing Group
DOI: 10.1038/s41592-020-0859-z

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)

Author(s): Marine H. Laporte, Nikolai Klena, Virginie Hamel & Paul Guichard
Published in: Nature Methods, 2022, ISSN 1548-7091
Publisher: Nature Publishing Group
DOI: 10.1038/s41592-021-01356-4

Architecture of the centriole cartwheel‐containing region revealed by cryo‐electron tomography

Author(s): Nikolai Klena, Maeva Le Guennec, Anne‐Marie Tassin, Hugo van den Hoek, Philipp S Erdmann, Miroslava Schaffer, Stefan Geimer, Gabriel Aeschlimann, Lubomir Kovacik, Yashar Sadian, Kenneth N Goldie, Henning Stahlberg, Benjamin D Engel, Virginie Hamel, Paul Guichard
Published in: The EMBO Journal, Issue 39/22, 2020, ISSN 0261-4189
Publisher: Nature Publishing Group
DOI: 10.15252/embj.2020106246